The potential physiological implications of polygalacturonic acid-mediated production of superoxide

Spasojević, Ivan; Pristov, Jelena Bogdanović

doi:10.4161/psb.5.12.12838

Cited by 7 publications

(3 citation statements)

References 51 publications

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“…We have shown recently that pectin and its constituent PGA have the ability to produce ⋅ O 2 – in the presence of ⋅ OH. This property was not observed for other cell wall carbohydrates – cellulose and hemicellulose (Spasojević and Bogdanović Pristov ). UV is known to provoke the production of ⋅ OH via the homolysis of hydrogen peroxide (H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 87%

UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid

et al. 2012

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We examined the redox effects of UV irradiation on cell wall isolates from Pisum sativum leaves, and polygalacturonic and galacturonic acid, in the presence of hydrogen peroxide. For this purpose, electron paramagnetic resonance spectroscopy and two spin-traps (DEPMPO and BMPO), capable of differentiating between various free radicals, were applied. Systems were exposed to UV-B (maximum emission at 312 nm) and UV-A (352 nm) for 10 min (6 J m(-2) s(-1)). Cell wall isolates exposed to UV in the presence of hydrogen peroxide, produced hydroxyl radical, carbon dioxide radical and superoxide. The production of superoxide was observed for cell wall isolates, polygalacturonic acid (in the presence and in the absence of calcium) and galacturonic acid, and it was diminished upon superoxide dismutase supplementation. The production is at least partially based on the reaction of hydroxyl radicals with (poly)galacturonic acid having carbon dioxide radicals as a products. Acting as a strong reducing agent, carbon dioxide radical reacts with molecular oxygen to produce superoxide. The results presented here shed a new light on: (1) the redox-modulating role of cell wall; (2) the production of superoxide in the extracellular compartment; (3) the mechanisms involved in translating UV stress into molecular signaling and (4) some other UV-related phenomena in plants, such as CO(2) emission.

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Section: Introductionmentioning

confidence: 87%

UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid

et al. 2012

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“…The CW plasma membrane interface is hypothesized as a key site for the stress signal perception where the interaction was studied between arabinogalactan proteins (AGPs) and receptor like kinases (Baluška et al, 2003 ). The production of hydrogen peroxide and downward redox signaling during the stress is an interesting aspect of CWPs (Spasojević and Pristov, 2010 ). The generation of reactive oxygen species (ROS) in response to CWP signaling (Barceló and Laura, 2009 ) is an important and interesting phenomenon, because mitochondria and chloroplast are considered as the major players of ROS production (Voothuluru and Sharp, 2013 ).…”

Section: Cell Wall Proteins (Cwps)mentioning

confidence: 99%

Gene Mining for Proline Based Signaling Proteins in Cell Wall of Arabidopsis thaliana

et al. 2017

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The cell wall (CW) as a first line of defense against biotic and abiotic stresses is of primary importance in plant biology. The proteins associated with cell walls play a significant role in determining a plant's sustainability to adverse environmental conditions. In this work, the genes encoding cell wall proteins (CWPs) in Arabidopsis were identified and functionally classified using geneMANIA and GENEVESTIGATOR with published microarrays data. This yielded 1605 genes, out of which 58 genes encoded proline-rich proteins (PRPs) and glycine-rich proteins (GRPs). Here, we have focused on the cellular compartmentalization, biological processes, and molecular functioning of proline-rich CWPs along with their expression at different plant developmental stages. The mined genes were categorized into five classes on the basis of the type of PRPs encoded in the cell wall of Arabidopsis thaliana. We review the domain structure and function of each class of protein, many with respect to the developmental stages of the plant. We have then used networks, hierarchical clustering and correlations to analyze co-expression, co-localization, genetic, and physical interactions and shared protein domains of these PRPs. This has given us further insight into these functionally important CWPs and identified a number of potentially new cell-wall related proteins in A. thaliana.

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“…promoted by chemically processed pectin from apple by approximately 65% in comparison to processed polygalacturonic acid and citrus pectin, might be the main cause of the antimicrobial activity of the apple pectin derivative. It has been previously observed that Pristov, Jovanović, Mitrović,& Spasojević, 2013;Spasojević & Bogdanović Pristov, 2010). It has been documented that pectin and HO • react to produce the pectin C(5) radical, which further reacts with O 2 , thus forming a C(5)peroxyl radical.…”

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confidence: 96%

Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus

Martinov

Krstić

Spasić

et al. 2017

Food Research International

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Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosaccharides on hydroxyl radical (HO)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary-relevant HO-generating system (iron+ascorbate). The oligosaccharides react with HO radical to produce carbon dioxide radical anion (CO). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO, which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO.

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The potential physiological implications of polygalacturonic acid-mediated production of superoxide

Cited by 7 publications

References 51 publications

UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid

UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid

Gene Mining for Proline Based Signaling Proteins in Cell Wall of Arabidopsis thaliana

Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus

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