The Potential Revolution of Cancer Treatment with CRISPR Technology

Abstract

Immuno-oncology (IO) and targeted therapies, such as small molecule inhibitors, have changed the landscape of cancer treatment and prognosis; however, durable responses have been difficult to achieve due to tumor heterogeneity, development of drug resistance, and adverse effects that limit dosing and prolonged drug use. To improve upon the current medicinal armamentarium, there is an urgent need for new ways to understand, reverse, and treat carcinogenesis. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) 9 is a powerful and efficient tool for genome editing that has shown significant promise for developing new therapeutics. While CRISPR/Cas9 has been successfully used for pre-clinical cancer research, its use in the clinical setting is still in an early stage of development. The purpose of this review is to describe the CRISPR technology and to provide an overview of its current applications and future potential as cancer therapies.

Evaluation of oyster mushroom (Pleurotus ostreatus)-derived anthraquinone on the induction of apoptosis and suppression of MMP-2 and MMP-9 expression in breast cancer cells

“…Viral-mediated delivery of drugs by optimizing genes using CRISPR is a recent technology used to treat cancer 29 . Thus, these gene knockout sequences will pave the way for the use of therapeutic drugs to treat various cancers 30 - 32 .…”

Viral delivery of CRISPR/Cas9 genome editing for rapid crop improvement: A promising approach to enhance crop resilience against biotic and abiotic stresses

“…Cancer Therapeutics: CRISPR/Cas9 delivered via viral vectors has been explored as a potential cancer treatment. Studies have demonstrated the successful knockout of oncogenes in cancer cells, inhibiting tumor growth and metastasis (Stefanoudakis et al, 2023) [113] . Additionally, CRISPR/Cas9 has been used to enhance the targeting of cancer cells, increasing the specificity and effectiveness of chemotherapy (Balon et al, 2022) [13] .…”

“…2) We also address the challenges and considerations of this technology, such as off-target effects and regulatory implications, to facilitate its responsible and effective deployment in crop breeding. Advancements in CRISPR technologies have further expanded the toolbox for plant genome editing and transcriptional regulation (Lowder et al, 2015) [75] ; (Wada et al, 2022) [113] ; (Nambiar et al, 2022) [80] . For example, the Cas13a protein has been incorporated, allowing for precise epigenome editing in plants (Dreissig et al, 2017) [36] .…”

From Diagnosis to Treatment: Exploring the Latest Management Trends in Cervical Intraepithelial Neoplasia

“…Gene editing technologies aim to restore normal cellular function and halt the progression of CIN. The specificity and precision of these approaches are particularly appealing, as they can potentially spare healthy tissue and minimize adverse effects associated with more invasive treatments [ 70 ].…”