The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation

Zhang, Wei; Chen, Qiangqiang; Xu, Liaoyi; Cai, Jianfeng; Zhang, Jinzhi

doi:10.1016/j.gene.2020.145228

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…The psma4 can regulate insulin sensitivity ( 81 ). The psma6 , mat2b , and psmb4 play essential roles in adipogenesis ( 82 , 83 ). The acadl , cpt1 , and cpt2 are thought to be involved in lipolysis ( 84 – 86 ).…”

Section: Discussionmentioning

confidence: 99%

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Huang,

Deng,

Yang

et al. 2024

Front. Endocrinol.

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BackgroundMelanocortin-3 and -4 receptors (MC3R and MC4R), G protein-coupled receptors, play vital roles in the regulation of energy homeostasis. To understand the functions of mc3r and mc4r in the energy homeostasis of red crucian carp (Carassius auratus red var., RCC), we cloned mc3r and mc4r, analyzed the tissue expression and localization of the genes, and investigated the effects of knockout of mc3r (mc3r+/-) and mc4r (mc4r+/-) in RCC. ResultsThe full-length cDNAs of RCC mc3r and mc4r were 1459 base pairs (bp) and 1894 bp, respectively. qRT-PCR indicated that mc3r and mc4r were profusely expressed in the brain, but lower expressed in the periphery tissues. ISH revealed that mc3r and mc4r were located in NPP, NPO, NAPv, NSC, NAT, NRL, NLTl, and NLTp of the brain, suggesting that mc3r and mc4r might regulate many physiological and behavioral aspects in RCC. To further verify the roles of mc3r and mc4r in energy homeostasis, the mc3r+/- and mc4r+/- fish were obtained by the CRISPR/Cas9 system. The average body weights, total lengths, body depths, and food intake of mc4r+/- fish were significantly higher than those of mc3r+/- and the normal wild-type (WT) fish, but there was no difference between the mc3r+/- and WT fish, indicating that the RCC phenotype and food intake were mainly influenced by mc4r but not mc3r. Interestingly, mc4r+/- fish displayed more visceral fat mass than mc3r+/- and WT fish, and mc3r+/- fish also exhibited slightly more visceral fat mass compared to WT. RNA-seq of the liver and muscle revealed that a large number of differentially expressed genes (DEGs) differed in WT vs. mc3r+/-, WT vs. mc4r+/-, and mc3r+/- vs. mc4r+/-, mainly related to lipid, glucose, and energy metabolism. The KEGG enrichment analysis revealed that DEGs were mainly enriched in pathways such as steroid biosynthesis, fatty acid metabolism, fatty acid biosynthesis, glycolysis/gluconeogenesis, wnt signaling pathway, PPAR signaling pathway, and MAPK signaling pathway, thereby affecting lipid accumulation and growth. ConclusionIn conclusion, these results will assist in the further investigation of the molecular mechanisms in which MC3R and MC4R were involved in the regulation of energy homeostasis in fish.

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Section: Discussionmentioning

confidence: 99%

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Huang,

Deng,

Yang

et al. 2024

Front. Endocrinol.

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“…Preadipocyte proliferation is controlled by a complex network of transcription factors [4][5][6]. For instance, BMP4 upregulates the proliferation of ICP1 cells by promoting the G1/S transition [44], whereas ALDH1A1 is a negative regulator of preadipocyte proliferation and inhibits the G1/S transition [45].…”

Section: Discussionmentioning

confidence: 99%

“…Adipocyte number mainly attribute to preadipocyte proliferation, whereas adipocyte size attribute to preadipocyte differentiation [3]. Preadipocyte proliferation is a complex process controlled by a regulatory network of multiple factors, including transcription factors [4][5][6], cytokines [7], and non-coding RNAs [8,9].…”

Section: Introductionmentioning

confidence: 99%

KLF7 Promotes Preadipocyte Proliferation via Activation of the Akt Signaling Pathway by Cis-regulating CDKN3

Jia

Jin

et al. 2022

Preprint

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Kruppel-like transcription factor 7 (KLF7) promotes preadipocyte proliferation; however, its target gene in this process has not yet been identified. Using KLF7 ChIP-seq analysis, we previously showed that a KLF7-binding peak is present upstream of the cyclin-dependent kinase inhibitor 3 gene (CDKN3) in chicken preadipocytes. In the current study, we identified CDKN3 as a target gene of KLF7 that mediates the effects of KLF7 on preadipocyte proliferation. Furthermore, 5 ′ -truncating mutation analysis showed that the minimal promoter was located between CDKN3 nt -160 and nt -7 (relative to the translation initiation codon ATG). KLF7 overexpression increased CDKN3 promoter activity in the DF-1 and immortalized chicken preadipocyte (ICP1) cell lines. Deletion of the putative binding site of KLF7 abolished the promotive effect of KLF7 overexpression on CDKN3 promoter activity. Moreover, CDKN3 -knockdown and -overexpression assays revealed that CDKN3 enhanced ICP1 cell proliferation. Flow cytometry analysis showed that CDKN3 accelerated the G1/S transition. Further, we found that KLF7 promoted ICP1 cell proliferation via Akt phosphorylation by regulating CDKN3. Taken together, these results suggest that KLF7 promotes preadipocyte proliferation via activating the Akt signaling pathway by cis – regulating CDKN3, thus driving the G1/S transition.

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“…Since PSMA6 functions as a crucial subunit of the 20S proteasome core complex, [40][41][42] we next explored how OA nanomicelles mediated proteasome activity via PSMA6. As PSMA6 plays a noncatalytic role in proteasome, we hypothesized that OA nanomicelles induced PSMA6 conformational changes to interfere with the stacking of two inner α-rings in 20S proteasome.…”

Section: Oa Nanomicelles Dynamically Regulate Psma6 Conformational Ch...mentioning

confidence: 99%

Inherent Capability of Self‐Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis

Luo

et al. 2022

Small

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Understanding the direct interaction of nanostructures per se with biological systems is important for biomedical applications. However, whether nanostructures regulate biological systems by targeting specific cellular proteins remains largely unknown. In the present work, self‐assembling nanomicelles are constructed using small‐molecule oleanolic acid (OA) as a molecular template. Unexpectedly, without modifications by functional ligands, OA nanomicelles significantly activate cellular proteasome function by directly binding to 20S proteasome subunit alpha 6 (PSMA6). Mechanism study reveals that OA nanomicelles interact with PSMA6 to dynamically modulate its N‐terminal domain conformation change, thereby controlling the entry of proteins into 20S proteasome. Subsequently, OA nanomicelles accelerate the degradation of several crucial proteins, thus potently driving cancer cell pyroptosis. For translational medicine, OA nanomicelles exhibit a significant anticancer potential in tumor‐bearing mouse models and stimulate immune cell infiltration. Collectively, this proof‐of‐concept study advances the mechanical understanding of nanostructure‐guided biological effects via their inherent capacity to activate proteasome.

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The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation

Cited by 6 publications

References 40 publications

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

KLF7 Promotes Preadipocyte Proliferation via Activation of the Akt Signaling Pathway by Cis-regulating CDKN3

Inherent Capability of Self‐Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis

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