The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes

Yang, Yang; Fei, Mengxue; Zhou, Xinying; Li, Yuejun; Jin, Dadi

doi:10.1080/21691401.2019.1611593

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Huang et al (29) confirmed that the lncRNA small nucleolar RNA host gene 4 can promote OS proliferation and migration by sponging miR-377-3p. Furthermore, Yang et al (30) revealed that lncRNA-BC050642 could promote OS cell proliferation, stimulate colony formation and inhibit cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA C5orf66‑AS1 inhibits osteosarcoma cell proliferation and invasion via miR‑149‑5p upregulation

Zhang

Song

2021

Oncol Lett

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Osteosarcoma (OS) is the most common primary malignant bone tumor in the pediatric age group. Despite the various potential treatments for OS, the cure rate of patients with OS remains very low. An increasing number of long non-coding RNAs (lncRNAs) have been identified as key regulators of the progression of malignant human tumors. However, the biological functions of the lncRNA C5orf66-antisense 1 (C5orf66-AS1) in OS are yet to be fully elucidated. The present study aimed to investigate the functions and underlying mechanisms of C5orf66-AS1 in OS tissues and cell lines. Expression levels of C5orf66-AS1 and microRNA (miRNA/miR)-149-5p in tissues from patients with OS and OS cells lines were evaluated using reverse transcription quantitative (RT-q)PCR. The miRNA target interaction between C5orf66-AS1 and miR-149-5p was predicted and verified using StarBase and dual-luciferase reporter assays. Cell viability, migration, invasion and apoptosis were analyzed using Cell Counting Kit-8, Transwell assays and flow cytometry, respectively. In addition, the expression levels of migration-and apoptosis-associated proteins [matrix metalloproteinase-9 (MMP-9), Bcl-2 and Bax] were determined using western blotting and RT-qPCR. The results demonstrated that C5orf66-AS1 was significantly upregulated and miR-149-5p was significantly downregulated in OS tissues and cells (MG63 and U2OS). Bioinformatics analysis further confirmed that miR-149-5p could directly bind to C5orf66-AS1. Furthermore, it was revealed that C5orf66-AS1 negatively regulated the expression of miR-149-5p in OS cells, as confirmed by the inhibition of C5orf66-AS1 expression and miR-149-5p upregulation in cells transfected with small interfering (si RNA targeting C5orf66-AS1. In addition, C5orf66-AS1 silencing significantly inhibited the proliferation, invasion and migration of U2OS cells, and stimulated cell apoptosis. These findings were reversed using miR-149-5p inhibitor. Increased Bax expression and decreased Bcl-2 and MMP-9 expression were also observed in C5orf66-AS1-siRNA transfected U2OS cells, compared with the control group. In summary, the results from the present study indicated that C5orf66-AS1 knockdown inhibits OS cell proliferation and invasion via the upregulation of miR-149-5p. This findings may provide a promising novel target for the treatment of OS.

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Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA C5orf66‑AS1 inhibits osteosarcoma cell proliferation and invasion via miR‑149‑5p upregulation

Zhang

Song

2021

Oncol Lett

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“…Research shows that lncRNAs have a substantial impact on cancer metastasis and medication sensitivity. For instance, LncRNA BC050642 is found to be considerably more abundant in OS tissues and cell lines, and it was also proposed as an independent biomarker of OS prognosis ( Yang Y. et al, 2019 ). The highly elevated LINC01614 in OS tissues predicts a poorer prognosis for patients.…”

Section: Potential Application Of Lncrnas In Osmentioning

confidence: 99%

Emerging roles of long non-coding RNAs in osteosarcoma

Liao,

Wei,

Zhou

et al. 2024

Front. Mol. Biosci.

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Osteosarcoma (OS) is a highly aggressive and lethal malignant bone tumor that primarily afflicts children, adolescents, and young adults. However, the molecular mechanisms underlying OS pathogenesis remain obscure. Mounting evidence implicates dysregulated long non-coding RNAs (lncRNAs) in tumorigenesis and progression. These lncRNAs play a pivotal role in modulating gene expression at diverse epigenetic, transcriptional, and post-transcriptional levels. Uncovering the roles of aberrant lncRNAs would provide new insights into OS pathogenesis and novel tools for its early diagnosis and treatment. In this review, we summarize the significance of lncRNAs in controlling signaling pathways implicated in OS development, including the Wnt/β-catenin, PI3K/AKT/mTOR, NF-κB, Notch, Hippo, and HIF-1α. Moreover, we discuss the multifaceted contributions of lncRNAs to drug resistance in OS, as well as their potential to serve as biomarkers and therapeutic targets. This review aims to encourage further research into lncRNA field and the development of more effective therapeutic strategies for patients with OS.

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“…LncRNAs can play important roles in drug sensitivity and cancer metastasis. lncR-BC050642 was significantly increased in OS tissues and cell lines, playing an important role in promoting the proliferation of OS cells, who can also be an independent biomarker of OS prognosis ( Yang et al, 2019 ). In OS tissues, the significantly upregulated lncR-01614 indicated a worse prognosis for patients.…”

Section: Prognostic Biomarkersmentioning

confidence: 99%

Non-coding RNAs as potential biomarkers in osteosarcoma

et al. 2022

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Osteosarcoma (OS) is a primary solid malignant tumor that occurs most frequently in the metaphysis of long bones. More likely to happen to children and adolescents. OS has high mortality and disability rate. However, the etiology and pathogenesis of OS have not been fully understood till now. Due to the lack of effective biomarkers, OS cannot be precisely detected in the early stage. With the application of next-generation and high-throughput sequencing, more and more abnormally expressed non-coding RNAs(ncRNAs) have been identified in OS. Growing evidences have suggested the ncRNAs, such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), have played an important role in the tumorigenesis and progression of OS. Thus, they can be served as novel biomarkers for diagnosis, treatment and prognosis. This review summarized the application of ncRNA as biomarkers in OS in detail, and discussed the limitation and future improvement of the potential biomarkers.

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The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes

Cited by 5 publications

References 39 publications

Knockdown of lncRNA C5orf66‑AS1 inhibits osteosarcoma cell proliferation and invasion via miR‑149‑5p upregulation

Knockdown of lncRNA C5orf66‑AS1 inhibits osteosarcoma cell proliferation and invasion via miR‑149‑5p upregulation

Emerging roles of long non-coding RNAs in osteosarcoma

Non-coding RNAs as potential biomarkers in osteosarcoma

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