The development of the microalgae-based biodies el technology has become a hot research topic in the bioenergy field in recent years. Presently, the technical possibility of the conversion of microalgae to biodiesel has been confirmed at the labo ratory scale. The fundamental issues impeding the industrialization of microalgae-based biodiesel include the high cost of production and the lack of research on the scaling-up technology. In this paper, the technical challenges and economic aspects of biodiesel production from microalgae were analyzed. It was found that the production cost of microalgae -based biodiesel mainly come from three processes: microalgae cultivation, harvest, and lipid extraction, among which microalgae cultivation represented t he highest cost. Finally, the prospect of the industrialization of the microalgae-based biodiesel was proposed.