The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects

Borai, Anwar; Khalil, Haitham; Alghamdi, Basma; Alhamdi, Raghad; Ali, Najwa; Bahijri, Suhad; Ferns, Gordon A.

doi:10.1002/jcla.23037

Cited by 9 publications

(6 citation statements)

References 13 publications

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“…As such, there could be variability of plasma levels regarding the storing period. However, studies confirmed that plasma vitamin D levels can be stable after long-term storage at -20°C [17]. Thus, this restriction will not affect our results.…”

Section: Limitations Of the Studymentioning

confidence: 58%

Sun exposure, dressing habits, and vitamin D status in Morocco

et al. 2021

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Sunlight is the main source of vitamin D. Almost 80% of vitamin D3 is produced in the skin. The region of Draa Tafilalet benefits from long daily sunshine time. As such, vitamin D deficiency should be low in this region. However, dressing habits can highly influence vitamin D status. This study aimed to evaluate sun exposure and its relation to vitamin D status among the general population in the region of Draa-Tafilalet, Morocco. Clinical, biological, and sociodemographic data were obtained from 331 adults visiting local healthcare units during the summers of 2019 and 2020. Plasma 25(OH)D2/D3 was measured by immunofluorescence. The prevalence of vitamin D deficiency was 37.5%, while Vitamin D insufficiency represented 56.5%. Most of the studied population (76.4%) had restricting dressing habits and exposed only their faces. statistic tests showed a correlation of vitamin D levels with daily sun exposure (r= 0.308, p<0.001), dressing habits (p<0.001), age (r= -0.141, p=0.01), sex (p<0.001), physical activity level (p=0.003), and BMI (r= -0.298, p<0.001). The study showed a high prevalence of vitamin D deficiency in this region. Similarly, it exposed the effects of restricting clothes and low daily sun exposure on vitamin D status.

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Section: Limitations Of the Studymentioning

confidence: 58%

Sun exposure, dressing habits, and vitamin D status in Morocco

et al. 2021

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“…Different storage conditions (fresh samples vs. up to 24 h at room temperature, different centrifuging times/temperature, multiple freeze–thaw cycles) did not significantly affect 25(OH)D values [ 7 , 14 , 16 , 17 , 18 , 19 ]. Long-term stability of 25(OH)D at −20 °C and −80 °C is generally acceptable, although a 15% variation after two months [ 20 ] or significant loss after four months at −20 °C was observed [ 14 ].…”

Section: Methodological Determinantsmentioning

confidence: 99%

Vitamin D Determinants, Status, and Antioxidant/Anti-inflammatory-Related Effects in Cardiovascular Risk and Disease: Not the Last Word in the Controversy

et al. 2023

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Beyond its key role in calcium homeostasis, vitamin D has been found to significantly affect the cardiovascular (CV) system. In fact, low vitamin D levels have been associated with increased CV risk, as well as increased CV morbidity and mortality. The majority of effects of this molecule are related directly or indirectly to its antioxidative and anti-inflammatory properties. Generally, vitamin D insufficiency is considered for 25-hydroxyvitamin D (25(OH)D) levels between 21–29 ng/mL (corresponding to 52.5–72.5 nmol/L), deficiency as 25(OH)D levels less than 20 ng/mL (<50 nmol/L), and extreme deficiency as 25(OH)D less than 10 ng/mL (<25 nmol/L). However, the definition of an optimal vitamin D status, as defined by 25(OH)D, remains controversial for many extra-bone conditions, including CV disease. In this review, confounding factors affecting the 25(OH)D measurement and status will be discussed. In particular, available evidence on the mechanism and role of vitamin D in relation to CV risk and disease through its antioxidant effect will also be reported, also facing the aspect regarding the debate on the minimum blood 25(OH)D level required to ensure optimal CV health.

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“…Preanalytical stability of serum 25-hydroxyvitamin D following long-term sample storage at up to −40 °C has been demonstrated previously. 23 , 24 …”

Section: Methodsmentioning

confidence: 99%

Pretreatment Vitamin D Concentrations Do Not Predict Therapeutic Outcome to Anti-TNF Therapies in Biologic-Naïve Patients With Active Luminal Crohn’s Disease

Chanchlani

Lin

Smith

et al. 2023

Crohn's &Amp; Colitis 360

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Background and Aims Vitamin D has a regulatory role in innate and adaptive immune processes. Previous studies have reported that low pre-treatment vitamin D concentrations are associated with primary non-response (PNR) and non-remission to anti-TNF therapy. This study aimed to assess whether pre-treatment 25-hydroxyvitamin D concentrations predicted PNR and non-remission to infliximab and adalimumab in patients with active luminal Crohn’s disease. Methods 25-hydroxyvitamin D concentrations were measured in stored baseline samples from 659 infliximab- and 448 adalimumab-treated patients in the Personalised Anti-TNF Therapy in Crohn’s disease (PANTS) study. Cut-offs for vitamin D were: deficiency < 25nmol/L, insufficiency 25-50nmol/L and adequacy/sufficiency > 50nmol/L. Results 17.1% (189/1107; 95% confidence interval [CI] 15.0 - 19.4%) and 47.7% (528/1107; 95% CI 44.8 - 50.6%) patients had vitamin D deficiency and insufficiency, respectively. 22.2% (246/1107) patients were receiving vitamin D supplementation. Multivariable analysis confirmed that sampling during non-summer months, South Asian ethnicity, lower serum albumin concentrations and nontreatment with vitamin D supplements were independently associated with lower vitamin D concentrations. Pre-treatment vitamin D status did not predict response or remission to anti-TNF therapy at week 14 (infliximab Ppnr = 0.89, adalimumab Ppnr = 0.18) or non-remission at week 54 (infliximab p = 0.13, adalimumab p = 0.58). Vitamin D deficiency was, however, associated with a longer time to immunogenicity in patients treated with infliximab, but not adalimumab. Conclusion Vitamin D deficiency is common in patients with active Crohn’s disease. Unlike previous studies, pre-treatment vitamin D concentration did not predict PNR to anti-TNF treatment at week 14 or non-remission at week 54.

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The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects

Cited by 9 publications

References 13 publications

Sun exposure, dressing habits, and vitamin D status in Morocco

Sun exposure, dressing habits, and vitamin D status in Morocco

Vitamin D Determinants, Status, and Antioxidant/Anti-inflammatory-Related Effects in Cardiovascular Risk and Disease: Not the Last Word in the Controversy

Pretreatment Vitamin D Concentrations Do Not Predict Therapeutic Outcome to Anti-TNF Therapies in Biologic-Naïve Patients With Active Luminal Crohn’s Disease

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