The precipitation reactions of normal serum and lipoid suspensions

Cardiolipin AntigenIII. An Examination of Specificity

“…Mackie and Anderson (1937) found thus that an acetone-soluble portion of sheep heart, which was discarded during the preparation of antigen, precipitated with sera from both syphilitic and non-syphilitic patients. In their opinion it is possible that biologically false positive sera contain large quantities of a reagin reacting with the impurities of the syphilis antigen.…”

“…* * The specificity was determined on the basis of questionnaires sent to doctors and hospitals and data obtained from the Central Register of Syphilitic Patients (Jersild, 1919 ;Madsen and Krag, 1937;. * 31 sera (1 6 per cent.)…”

Serology of Syphilis based on Recent Observations

“…But any such reactions were looked upon as " false positives ". In this connexion an extensive study by Mackie and Anderson (1937) may 136 be mentioned. These workers reported three different reacting zones with human and animal sera by the use of lipid suspensions from various sources (sheep heart, B. diphtheria, and lecithins).…”

Ford Robertson and Colquhoun Modification of the Meinicke Clarification Reaction Compared with the Harrison-Wyler Wassermann and the Standard Kahn Reactions

“…This phenomenon was well known in both M.K.R.1 and M.K.R.2, it being fully recognized by Meinicke. Colle (1946) has drawn attention to its practical disadvantage, and its mechanism has been studied by Dunlop and Sugden (1934), Mackie andAnderson (1937), andFord Robertson andColquhoun (1940). However, under a further analysis of the data obtained by Colquhoun, Kyles, and Rannie (1945), Ford Robertson (personal communication) found no false negative in 385 untreated cases of syphilis, but in 0-51 per cent.…”