The Preclinical Study of Hyeolbuchugeo-tang (Xuefuzhuyu-tang) on Bone Healing in Rats with Rib Fracture

Abstract

Objectives The purpose of this study is to evaluate the healing effect of Hyeolbuchugeotang (HC) in rats with rib fracture. Methods Rats were randomly divided into 5 groups (naive, control, positive control, HC-L and HC-H). All groups except naive group were subjected to bone fracture of rib. Naive group received no treatment at all. Control group was fed with phosphate buffered saline. Positive control group was orally medicated with tramadol. Experimental group was orally medicated with HC extract (50 mg/kg for low concentration [HC-L], 100 mg/kg for high concentration [HC-H]). X-ray and micro-computed tomography (micro-CT) were conducted to assess the effect of HC. We analysed the level of 2) transforming growth factor-β1 (TGF-β1), Ki67, alkaline phosphatase (ALP), receptor activator of nuclear factor kappa-β, runt-related transcription factor 2 (Runx2) and tartrate resistant acid phosphatase (TRAP) on 7 and 14 days after fracture. ALP, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine was measured for safety assessment. Results X-ray and micro-CT, showed HC enhance bone repair process. Callus formation was increased in experimental group at 7 days after fracture, but decreased at 14 days after fracture. 7 days after fracture, the level of TGF-β1 in experimental group was decreased. The level of Ki67, Runx2 in HC-H, TRAP in HC-L was increased. 14 days after fracture, the level of Ki67 in HC-L and HC-H was decreased. The level of ALP, Runx2, BUN in HC-L, TRAP in HC-L and HC-H was increased. Conclusions Taken together the results, HC promoted healing of bone fracture. In conclusion, HC has a potential to promote healing of bone fracture.

Long-term follow-up of inpatients with traumatic fractures who received integrative Korean Medicine treatment: A retrospective analysis and questionnaire survey study

“…Previous experimental studies on herbal medicine showed that callus formation occurred faster in the herbal medicine group than in the control group. [ 37 , 38 ] Measurement of radical scavenging activity of herbal extracts showed a dose-dependent increase in antioxidant activity and a significant decrease in the production of the inflammatory mediator interleukin-1β, indicating that herbal extracts can help relieve inflammatory responses accompanying fractures. [ 39 , 40 ] Osteogenesis during the reparative phase can be facilitated by bone morphogenetic protein type 2 that mediates the differentiation of osteoblasts and activates transforming growth factor-β1 and Sox9 that regulates the transcription of type 2 collagen.…”

Effects of Extracts from Cnidium officinale and Angelica sinensis on Bone Fusion in Mice with Femoral Fracture

“…현재까지 골절 치료를 위한 다방면의 한약 연구가 보 고되었다. 방제를 활용한 연구로는 加味芎歸湯 33) , 加味 身痛逐瘀湯 14,15) , 順氣活血湯 16) , 復元活血湯 17,18) , 當歸鬚 散 19,20) , 破血散疼湯 21) , 血府逐瘀湯 22) , 桃紅四物湯…”

“…한의학 서적 중《外臺秘要》에서는 "救急療救骨折, 接 令如故"로 골절에 대한 병명을 처음 언급하였고 7) , ≪醫 宗金鑑≫에서는 부위별 보호대를 활용하는 방법을 제시 하였으며 8) , ≪聖濟總錄≫에서는 골절치료에 한약을 초 기에는 活血祛瘀, 중기에는 接骨續筋, 후기에는 補氣養 血, 健壯筋骨 하는 시기별 방법을 설명하였다 9) . 요 구성 약재로 쓰였을 뿐만 아니라 13) , 골절 유합에 효과 적임을 보고한 복합약물연구 加味身痛逐瘀湯 14,15) , 順氣活 血湯 16) , 復元活血湯 17,18) , 當歸鬚散 19,20) , 破血散疼湯 21) , 血府 逐瘀湯 22) , 桃紅四物湯 23) 를 이용하여 5 mm 간격으로 단순 결찰 봉합하였다. 반 대쪽 다리도 같은 방법으로 골절을 유발하였다(Fig.…”