The Predominant Variant of the Brazilian Epidemic Clonal Complex of Methicillin‐Resistant<i>Staphylococcus aureus</i>Has an Enhanced Ability to Produce Biofilm and to Adhere to and Invade Airway Epithelial Cells

Amaral, Maribel M. Do; Coelho, L. R.; Flores, Rosania P.; Souza, Raquel Cristina de Souza e; Silva-Carvalho, Maria Cícera; Teixeira, Lenise Arneiro; Ferreira-Carvalho, Bernadete Teixeira; Figueiredo, Agnes Marie Sá

doi:10.1086/432515

Cited by 105 publications

(117 citation statements)

References 36 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…This is similar to what has been reported in other tertiary hospitals in Malaysia (26,33), China (41), Germany (42), and Russia (15). This Brazilian/Hungarian MRSA clone (MLST ST239) has advantageous genetic properties that enhance the ability of biofilm formation, leading to the adherence and invasion of human airway cells (43). Thirty-two (96z) of the ST239 MRSA strains were SCCmec type III, inferring the possibility that ST239 MRSA strains in this hospital might have evolved from ST239 MSSA strains via acquisition of the SCCmec mobile element.…”

Section: Discussionsupporting

confidence: 86%

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

et al. 2012

View full text Add to dashboard Cite

SUMMARY: Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. The objective of this study was to determine genetic relatedness between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. We isolated 35 MRSA and 21 MSSA strains from sporadic cases at the main tertiary hospital in Terengganu, Malaysia, screening them for the presence of virulence genes. Their genetic relatedness was determined by accessory gene regulator (agr) types, PCR-restriction fragment length polymorphism (RFLP) of the coa gene, pulsed-field gel electrophoresis (PFGE), S. aureus protein A (spa), and multilocus-sequence typing (MLST). We found that 57z of MRSA and 43z of MSSA strains harbored enterotoxin genes. The majority (87.5z) of the strains were agr type I. PCR-RFLP and PFGE genotyping of the coa gene revealed that MRSA strains were genetically related, whereas MSSA strains had higher heterogeneity. The combined genotype, MLST-spa type ST239-t037, was shared among MRSA and MSSA strains, indicating that MRSA strains could have evolved from MSSA strains. Two combined MLST-spa types were present in MRSA strains, whereas 7 different MLST-spa types were detected in MSSA strains, including 2 combined types (ST779-t878 and ST1179-t267) that have not been reported in Malaysia. In conclusion, enterotoxin genes were more prevalent in MRSA than in MSSA strains in the Terengganu hospital. The MSSA strains were genetically more diverse than the MRSA strains.

show abstract

Section: Discussionsupporting

confidence: 86%

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Croes et al suggested that MLST CC8 was a predisposing factor for a strain to produce strong biofilms (Croes et al, 2009). Furthermore, the Brazilian clone (also MLST CC8) was shown to have increased adherence (Amaral et al, 2005). The EMRSA-15 clone in Scotland was found to form stronger biofilms than EMRSA-16 isolates (Smith et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Naicker

Karayem

Hoek

et al. 2016

Microbial Pathogenesis

View full text Add to dashboard Cite

2 Declaration I, Preneshni Rochelle Naicker, hereby declare that the work contained in this assignment is my original work and that I have not previously submitted it, in its entirety or in part, at any university for a degree. The ability to form biofilms contributes significantly to the virulence of Staphylococcus aureus. Virulence factors may be associated with certain S. aureus lineages. However, the contribution of the genetic background of S. aureus to biofilm formation is poorly understood. This study investigated the association between the genetic background and the biofilm forming ability of clinical invasive S. aureus isolates. Secondary objectives included investigating any correlation with biofilm formation and methicillin resistance or the source of bacteraemia. Methods:The study was conducted at a 1300-bed tertiary hospital in Cape Town, South Africa. S. aureus isolates obtained from blood cultures between January 2010 and January 2012 were included. Genotypic characterization was performed by PFGE, spa typing, SCCmec typing and MLST. Thirty genotypically unique strains were assessed for phenotypic biofilm formation with the microtitre plate assay. All isolates were tested in triplicate and an average optical density, measured at a wavelength of 490nm, was determined. The biofilm forming ability of isolates with A 490 > 0.17 was considered 'weak positive' and A 490 > 0.34 'strong positive'. Isolates with A 490 ≤ 0.17 were considered non-adherent. ANOVA with Bonferroni-adjusted post-hoc tests and t-tests were used for statistical analysis of the association between biofilm forming ability and strain characteristics.Results: Fifty seven percent of isolates were capable of forming biofilms. Weak biofilm formation occurred in 40% (n=12) and strong biofilm formation in 17% (n=5) of isolates. Thirteen (43%) of the isolates were classified as non-adherent. All 5 isolates capable of strong biofilm formation belong to one spa clonal complex (spa-CC 064). Strains from spa-CC 064 were capable of higher biofilm formation than other spa clonal complexes (p=0.00002). These 5 strains belonged to MLST CC5 and CC8. Biofilm formation did not correlate with methicillin resistance and was not related to the source of bacteraemia. Conclusion:Biofilm formation correlates with the spa clonal lineage in our population of invasive S. aureus strains. High biofilm formation was associated with spa-CC 064. MLST CC5 and CC8 strains are capable of strong biofilm formation.

show abstract

“…Biofilms and hemolysis: Biofilms were detected with the microtiter plate method [11] and the biofilm unit calculated according to Amaral et al [12]. Briefly, assays were performed in triplicate in TSB/1% glucose in 96-well polystyrene flat-bottom tissue culture plates.…”

Section: Detection Of Virulence Determinantsmentioning

confidence: 99%

“…Isolates with BU > 2x the negative control value were considered biofilm producers and were classified as follows: weak, 0.182 < BU < 0.364; moderate, 0.364 < BU < 0.728; strong, BU > 0.728 [12]. Isolates M1 to M30 were from the ICU, while M31 to M50 were from the community.…”

mentioning

confidence: 99%

Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda

Okee

Joloba

Okello

et al. 2011

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Staphylococcus epidermidis is often considered a non-pathogenic organism but it causes nosocomial infections. To distinguish invasive strains, comparative studies of patient and community isolates may offer some clues. We investigated the distribution of virulence determinants in patient isolates from Uganda. Methodology: S. epidermidis isolates were identified with the Staph API ID 32 kit. Antimicrobial susceptibility, biofilm formation and hemolysis were detected with standard procedures. Genes associated with virulence (aap, atlE, bhp, hla, hld, ica, IS256, sdrE, sea, tsst) and antimicrobial resistance (aac(6')-Ie-aph(2'')-Ia, aph(3')- IIIa, blaZ, mecA, vanA/vanB1) were detected by PCR. Results: S. epidermidis grew in 30 (30/50, 60%) ICU samples and 20 (20/60, 33%) community samples (one isolate per sample per patient/person). All ICU isolates (30/30, 100%) were IS256 and hld positive, 22 (22/30, 73%) were biofilm/ica positive, 21 (21/30, 70%) were hemolytic on blood agar, nine (9/30, 30%) contained atlE gene, six (6/30, 20%) hla gene, five (5/30, 17%) aap gene, and three (3/30, 10%) bhp gene. A gene encoding an aminoglycoside-modifying enzyme, aph(3')-IIIa, was highly prevalent (28/30, 93%), while blaZ (2/30, 7%), mecA (3/30, 10%), vanA (3/30, 10%) and vanB1 (3/30, 10%) were less prevalent. Of the community isolates, one (1/20, 5%) was ica positive, two (2/20, 10%) formed biofilms, and three (3/20, 15%) possessed the atlE gene. bhp, aap, IS256, hld and antimicrobial resistance genes were not detected in community isolates. Conclusions: S. epidermidis from ICU patients in Mulago Hospital is potentially virulent and could be a reservoir for antimicrobial resistant genes.

show abstract

The Predominant Variant of the Brazilian Epidemic Clonal Complex of Methicillin‐ResistantStaphylococcus aureusHas an Enhanced Ability to Produce Biofilm and to Adhere to and Invade Airway Epithelial Cells

Cited by 105 publications

References 36 publications

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Prevalence of virulence determinants in Staphylococcus epidermidis from ICU patients in Kampala, Uganda

Contact Info

Product

Resources

About