The Preparation and Measurement of the Concentration of Dilute Bacterial Aerosols.

Ferry, Ronald M.; Farr, L.; Hartman, Mary G.

doi:10.1021/cr60138a010

Cited by 17 publications

(7 citation statements)

References 44 publications

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“…Of historical significance, the use of Bacillus globigii (BG) aerosol as a bacterial spore simulant was first mentioned by Gucker et al [22]. Being an electronics engineer, he had to rely on scientists at Harvard University to develop the BG aerosol dissemination procedure [26]. For aerosol generation, a medical nebulizer was used.…”

Section: Early Attempts Using Light Scatter Optical Particle Countingmentioning

confidence: 99%

History of the Early Biodetection Development

2014

Integrated Analytical Systems

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Section: Early Attempts Using Light Scatter Optical Particle Countingmentioning

confidence: 99%

History of the Early Biodetection Development

2014

Integrated Analytical Systems

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“…Although Koch had already developed objective image cytometry by means of photography, flow cytometry (FCM) really came to life in the late 1940s. Driven by the need to identify bacterial aerosols in warfare, the next generation of flow cytometers started with a design similar to Tyndall's (Gucker et al, 1947;Ferry et al, 1949;Gucker & O'Konski, 1949), the only difference being that the light source was now a Ford headlamp and detection was performed using a photodetector. The next period of more intensive FCM in microbiology was started in the mid-1970s by Hutter (1974); Hutter et al (1975), Paau et al (1977), Slater et al (1977) and Bailey et al (1977), and as early as 1979, the technology was already pushed to detect large viruses (Hercher et al, 1979).…”

Section: Introductionmentioning

confidence: 99%

Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities

2010

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The still poorly explored world of microbial functioning is about to be uncovered by a combined application of old and new technologies. Bacteria, especially, are still in the dark with respect to their phylogenetic affiliations as well as their metabolic capabilities and functions. However, with the advent of sophisticated flow cytometric and cell sorting technologies in microbiological labs, there is now the possibility to gain this knowledge at the single-cell level without cumbersome cultivation approaches. Cytometry also facilitates the understanding of physiological diversity in seemingly likewise acting populations. Both individuality and diversity lead to the complex and concerted actions of microbial consortia. This review provides an overview of the state of the art in the field. It deals with the handling of microorganisms from the very beginning (i.e. sampling, and detachment and fixation procedures) and goes on to discuss the pitfalls and problems in analysing cells without any further treatment. If information cannot be gained by specific staining procedures, phylogenetic technologies, transcriptomic and proteomic approaches may be options for achieving advanced insights. All in all, flow cytometry will be a mediator technology to gain a deeper insight into the heterogeneity of populations and the functioning of microbial communities.

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“…The Coulter counter was developed in the mid-1950s (11) and was adopted quickly. Photoelectric counting techniques were first proposed in the 1930s (35), but working devices were not available until the late 1940s (16,20,21). Technological developments of particle counters and flow cytometers continued through the 1960s, with particle counters gaining wide acceptance.…”

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confidence: 99%

Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry

DeLeo¹,

Baveye²

1996

Appl Environ Microbiol

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Flow cytometry was used to enumerate and characterize bacteria from a sand column microcosm simulating aquifer conditions. Pure cultures of a species of Bacillus isolated from subsurface sediments or Bacillus megaterium were first evaluated to identify these organisms' characteristic histograms. Counting was then carried out with samples from the aquifer microcosms. Enumeration by flow cytometry was compared with more-traditional acridine orange direct counting. These two techniques gave statistically similar results. However, counting by flow cytometry, in this case, surveyed a sample size 700 times greater than did acridine orange direct counting (25 l versus 0.034 l) and required 1/10 the time (2 h versus 20 h). Flow cytometry was able to distinguish the same species of bacteria grown under different nutrient conditions, and it could distinguish changes in cell growth patterns, specifically single cell growth versus chained cell growth in different regions of an aquifer microcosm. A biomass estimate was calculated by calibrating the total fluorescence of a sample from a pure culture with the dry weight of a freeze-dried volume from the original pure culture. Growth conditions significantly affected histograms and biomass estimates, so the calibration was carried out with cells grown under conditions similar to those in the aquifer microcosm. Costs associated with using flow cytometry were minimal compared with the amount of time saved in counting cells and estimating biomass.

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The Preparation and Measurement of the Concentration of Dilute Bacterial Aerosols.

Cited by 17 publications

References 44 publications

History of the Early Biodetection Development

History of the Early Biodetection Development

Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities

Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry

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