The Preparation, Antioxidant Activity Evaluation, and Iron-Deficient Anemic Improvement of Oat (Avena sativa L.) Peptides–Ferrous Chelate

Abstract

Iron-chelating peptides have been widely considered as one of the best iron supplements to alleviate the iron deficiency. In this study, a novel oat peptides–ferrous (OP-Fe2+) chelate was prepared from antioxidant oat peptides obtained in the laboratory of the authors. The optimal preparation condition was obtained through the single-factor and response surface methodology, and the chelating rate could reach up to 62.6%. After chelation, the OP-Fe2+ chelate exhibited a significantly higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity than oat peptides. It was discovered that the hemoglobin concentration and the number of red blood cell levels in OP-Fe2+-treated iron-deficient anemic (IDA) rats were significantly higher than untreated IDA rats. The OP-Fe2+ chelate could also improve the hypertrophy of the spleen, serum iron (SI), total iron and binding capacity, and serum ferritin levels in the IDA rats. In addition, the OP-Fe2+ treatment significantly increased the antioxidant activities of super oxidase and glutathione in the liver homogenate of the IDA rats. Therefore, the OP-Fe2+ chelate is an effective type of iron supplement for IDA rats, which could be a promising source with anti-anemia and antioxidant activity.

Effects of food-derived oligopeptide iron chelates on liver injury and gut microbiota homeostasis in iron-deficiency anemia female rats: a pilot study

“…36 When hepatocytes are damaged, AST and ALT are released into the circulatory system, resulting in elevated serum AST and ALT levels. 37 Similar to a previous study of IDA rats, 23 the serum ALT and AST levels of rats in the ID group were significantly increased in the current study, indicating liver injury. Intervention resulted in decreased serum ALT and AST to normal levels in each intervention group, suggesting that each of the different iron supplements chosen for our study may have a protective effect against liver injury caused by IDA.…”

“…36 When hepatocytes are damaged, AST and ALT are released into the circulatory system, resulting in elevated serum AST and ALT levels. 37 Similar to a previous study of IDA rats, 23 the serum ALT and AST levels of rats in the ID group were signifi-…”

“…On the 28 th day of the experiment, blood was collected from the tail vein for determining HGB levels, and rats with HGB < 100 g L −1 were considered to be IDA. 20,22,23 If the HGB standard was not met, the ID diet was continued. To ensure that there was no large difference in body weight at the start of the intervention, rats that had been on the ID diet for more than 35 days but still failed to meet the HGB standard were excluded.…”

Food-derived bioactive oligopeptide iron complexes ameliorate iron deficiency anemia and offspring development in pregnant rats

“…These peptides can form a stable structure with iron, potentially decreasing the Fe pro-oxidant effect that is responsible for damage of the gastrointestinal mucosa ( 20 ). Furthermore, peptide-Fe complexes can exert an indirect antioxidant capacity and neutralize lipid radicals ( 21 – 24 ). However, data of these potential effects in vivo remains limited.…”

Multiple Antioxidative and Bioactive Molecules of Oats (Avena sativa L.) in Human Health

“…So-called “superfoods” are food products that boost the immunity of the body through their rich contents of nutrients and antioxidants. Representative examples of superfoods include tomatoes, spinach, broccoli, salmon, garlic, and blueberries [ 1 ]. Recently, interest in so-called “functional grains”, which are also rich in nutrients and antioxidants, has increased, particularly in the United States of America, Europe, and Japan [ 2 ].…”