The preparation of glomeruli from renal biopsy specimens for scanning electron microscopy

Ng, Wing-Ling; So, Kwok-Fai; So, Pui-yiu; Ngai, H. K.

doi:10.3109/00313028209061381

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…As the filtration unit of the blood, the glomerulus itself has been an important subject for studying renal diseases. Single glomeruli were often isolated from the kidney for analysis of their ultrastructures [19] and omic profiles [20]. Motivated by this, we managed to dissect out single glomeruli from fixed porcine kidney tissues under a stereo microscope using tweezers.…”

Section: The Expansion Properties Of Isolated Renal Glomerulimentioning

confidence: 99%

Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks

Zhu

Wang

Chen

et al. 2021

Journal of Biophotonics

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Expansion microscopy has enabled super resolution imaging of biological samples. The accurate measurement of expansion factor and distortion typically requires locating and imaging the same region of interest in the sample before and after expansion, which is often time-consuming to achieve. Here we introduce a convenient method for relocation by utilizing isolated porcine glomeruli as landmarks during expansion. Following heat denaturation and proteinase K digestion protocols, the glomeruli exhibit expansion factor of 3.5 to 4 (only 7%-16% less expanded than the hydrogel), and 1% to 2% of relative distortion.Due to its appropriate size of 100 to 300 μm, the location of the glomerulus in the sample are visible to eyes, while its detailed shape only requires bright field microscopy. For expansion factors ranging from 3 to 10, the region in the vicinity of the glomerulus can be easily re-identified, and sometimes allows quantification of expansion factor and distortion under bright field without fluorescent labels.

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Section: The Expansion Properties Of Isolated Renal Glomerulimentioning

confidence: 99%

Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks

Zhu

Wang

Chen

et al. 2021

Journal of Biophotonics

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“…This technique, however, requires a significant amount of labor. In contrast, scanning electron microscopy (SEM) has provided information about the threedimensional morphology of the glomerulus -such as surface alterations of podocytes and endothelial cellswith processing that is easier and shorter than that of TEM (Arakawa, 1971, Arakawa andTokunaga, 1972;Jones, 1977;Tarpley and Williams, 1980;Yorioka 1980;Ng et al, 1982;Lahdenkari et al, 2004). However, investigation of the glomerular basement membrane (GBM), which is altered in response to subepithelial deposits in GN, has been difficult using conventional SEM in the secondly electron (SE) mode due to overlying cells.…”

Section: Methodsmentioning

confidence: 99%

Rapid three-dimensional analysis of renal biopsy sections by low vacuum scanning electron microscopy

Inaga

Kato

Hirashima

et al. 2011

Archives of Histology and Cytology

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and pathological features were investigated in every sample. The abnormal surface appearances of podocytes with foot processes and the varying thicknesses of GBM were revealed three-dimensionally, features difficult to observe under LM and transmission electron microscopy. PAM-positive GBM alterations in membranous GN were distinctly visualized through overlying cells without cell removal under LVSEM at high magnification. Not only prominent spike formation but also slight protrusions were clearly revealed in the side views of GBM. Craterlike or hole-like structures were shown in the en face views of GBM. Accordingly, LVSEM is expected to provide a novel approach to the pathological diagnosis of human glomerular diseases using conventional renal biopsy sections.

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“…Scanning and transmission mode electron microscopy of biopsies has been performed for several decades [118][119][120][121][122]. In our investigations, we have used FE-SEM on human biopsy tissue coming from various organs namely kidney [123][124][125][126], pancreas [127], skin [128][129][130][131], hairs [132], breast [26], thyroid [133,134], or arterial wall [135], but of course we must stress that such opportunities exist for cells and mice as well [136][137][138][139][140].…”

Section: Biopsy Imagingmentioning

confidence: 99%

Scanning electron microscopy—a powerful imaging technique for the clinician

Bazin¹,

Bouderlique²,

Daudon³

et al. 2022

Comptes Rendus. Chimie

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Since its first use several decades ago, scanning electron microscopy has been used in numerous investigations dedicated to biological systems. This contribution focuses on observations on pathological calcifications in order to review several major applications of primary importance to the clinician. Among these, we highlight such observations as medical diagnostic tools in pathologies arising from primary hyperoxaluria and urinary infections.

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The preparation of glomeruli from renal biopsy specimens for scanning electron microscopy

Cited by 11 publications

References 6 publications

Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks

Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks

Rapid three-dimensional analysis of renal biopsy sections by low vacuum scanning electron microscopy

Scanning electron microscopy—a powerful imaging technique for the clinician

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