The preparation of mitochondria from muscle without the use of a homogeniser

Bullock, Gillian; Carter, Elizabeth E.; White, Alan M.

doi:10.1016/0014-5793(70)80237-x

Cited by 42 publications

(10 citation statements)

References 3 publications

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“…Closely apposed membranes frequently were observed in ischemic mitochondrial preparations as well as in controls. This is a feature commonly encountered in isolated mitochondria and appeared to be nonspecific (Bullock et al, 1970). The structure of mitochondria isolated from hearts receiving palmitate is shown in Figure 3.…”

Section: Effects Of Substrate and Ischemia On The Mitochondrial Strucmentioning

confidence: 90%

Relationship between structure and fatty acid metabolism in mitochondria isolated from ischemic rat hearts.

Feuvray¹,

Plouët²

1981

Circulation Research

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We studied mitochondrial structure and intermediates of fatty acid metabolism in mitochondria isolated from ischemic hearts. By electron microscopy, no structural difference was detected between mitochondria isolated from control hearts and from ischemic hearts receiving glucose as the only substrate. However, major differences were observed in mitochondria obtained from control and ischemic hearts receiving both glucose and palmitate. These hearts contained a higher portion of damaged mitochondria. However, measurements of marker enzyme activities failed to show that more mitochondria were lost during the isolation procedure in ischemic than in control hearts. Many densely staining areas (or amorphous densities) were observed in the isolated mitochondria of ischemic hearts. These amorphous densities have an appearance similar to that observed in the intact ischemic heart under the same perfusion conditions. Levels of long-chain acyl-CoA in mitochondria isolated from hearts receiving glucose alone were practically the same for control and ischemic hearts and were only slightly increased in mitochondria of ischemic hearts receiving both glucose and palmitate. On the other hand, levels of long-chain acyl carnitine in mitochondria of ischemic hearts were twice those found in control hearts. The mitochondrial level of long-chain acyl carnitine was approximately four times higher in the ischemic hearts receiving palmitate compared to those receiving no palmitate. This rise in long-chain acyl carnitine levels in mitochondria isolated fom ischemic hearts receiving palmitate may be related to modifications of the mitochondrial structure and to the appearance of amorphous densities.

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Section: Effects Of Substrate and Ischemia On The Mitochondrial Strucmentioning

confidence: 90%

Relationship between structure and fatty acid metabolism in mitochondria isolated from ischemic rat hearts.

Feuvray¹,

Plouët²

1981

Circulation Research

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“…BCDH complex was purified partially from ox kidney mitochondria. The procedure was as in [ 1] incorporating the modification given in [8]. The final two steps in [1] (precipitations at pH 6.…”

Section: Methodsmentioning

confidence: 99%

“…The procedure was as in [ 1] incorporating the modification given in [8]. The final two steps in [1] (precipitations at pH 6. 8 and 6.4) Mitochondria were prepared from rat heart as in [lo], from rat liver and kidney as in [6] and from rat skeletal muscles with trypsin as in [ll].…”

Section: Methodsmentioning

confidence: 99%

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Activation of phosphorylated branched chain 2‐oxoacid dehydrogenase complex

1982

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“…The mitochondrial fraction was isolated by the method of Bullock et al [13] Mitochondrial function was determined by a polarographic method [14], using an OXY 5 polarograph-oxygraph (Gilson Medical Eletronics, Inc., W. Beltline Middeton, WI, USA). The respiration medium contained 0.23 M sucrose, 8 mM potassium phosphate, 9.5 mMTris, pH 7.0, 0.14 mg/ml albumin, and 1 mM EDTA.…”

Section: Mitochondrial Functionmentioning

confidence: 99%

Sanguineous normothermic, intermittent cardioplegia, effects on hypertrophic myocardium. Morphometric, metabolic and ultrastructural studies in rabbits hearts

Carbone¹,

Roselino²,

Mello³

et al. 2012

Revista Brasileira De Cirurgia Cardiovascular

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Sanguineous normothermic, intermittent cardioplegia, effects on hypertrophic myocardium. Morphometric, metabolic and ultrastructural studies in rabbits hearts Abstract Objectives: The present investigation aimed to study the protective effect of intermittent normothermic cardioplegia in rabbit's hypertrophic hearts.Methods: The parameters chosen were 1) the ratio heart weight / body weight, 2) the myocardial glycogen levels, 3) ultrastructural changes of light and electron microscopy, and 4) mitochondrial respiration.Results: 1) The experimental model, coarctation of the aorta induced left ventricular hypertrophy; 2) the temporal evolution of the glycogen levels in hypertrophic myocardium demonstrates that there is a significant decrease; 3) It was observed a time-dependent trend of higher oxygen consumption values in the hypertrophic group; 4) there was a significant time-dependent decrease in the respiratory coefficient rate in the hypertrophic group; 5) the stoichiometries values of the ADP: O2 revealed the downward trend of the values of the hypertrophic group; 6) It was possible to observe damaged mitochondria from hypertrophic myocardium emphasizing the large heterogeneity of data.Conclusion: The acquisition of biochemical data, especially the increase in speed of glycogen breakdown, when anatomical changes are not detected, represents an important result even when considering all the difficulties inherent in the process of translating experimental results into clinical practice. With regard to the adopted methods, it is clear that morphometric methods are less specific. Otherwise, the biochemical data allow detecting alterations of glycogen concentrations and mitochondria respiration before the morphometric alterations should be detected Descriptors: Heart arrest, induced. Hypertrophy, left ventricular. Cardiovascular surgical procedures. 622Rev Bras Cir Cardiovasc 2012;27(4):621- INTRODUCTIONThe current success of cardiac surgery was provided, among other relevant factors, by better understanding the myocardium protection. The timeline of this understanding includes: 1) systemic hypothermia [1]; 2) intra-operative cardiac arrest induced by potassium citrate [2]; 3) anoxic arrest of the heart [3]; 4) the use of chemicals that could rapidly determine cardiac arrest [4]; 5) Miscellaneous studies relating to the composition, temperature and mode of drug administration named cardioplegic solution [5][6][7], and; 6) normothermic cardioplegia [8,9].In cardiac hypertrophy, whether it is induced in an experimental model or observed clinically, energy metabolism is compromised highlighting that Sink et al.[10] demonstrated that the hypertrophic heart must be stopped immediately emphasizing the need of additives to the cardioplegia solution. It is noteworthy that Cooley et al., in 1972, described the "Stone Heart", i.e., ischemic contracture, more frequent and severe in the hypertrophic heart [11]. This ischemia/reperfusion phenomenon allowed the resumption of the discussion, and final conclusion, about the pivota...

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The preparation of mitochondria from muscle without the use of a homogeniser

Cited by 42 publications

References 3 publications

Relationship between structure and fatty acid metabolism in mitochondria isolated from ischemic rat hearts.

Relationship between structure and fatty acid metabolism in mitochondria isolated from ischemic rat hearts.

Activation of phosphorylated branched chain 2‐oxoacid dehydrogenase complex

Sanguineous normothermic, intermittent cardioplegia, effects on hypertrophic myocardium. Morphometric, metabolic and ultrastructural studies in rabbits hearts

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