To gain insight into the potential
for aerosolization of viruses
in wastewater systems, we investigated the partitioning of MS2 and
Phi6 bacteriophages in synthetic sludge and anaerobically digested
sludge from a wastewater treatment plant. We evaluated partitioning
among the liquid, solids, and material surfaces of porcelain, concrete,
polyvinyl chloride (PVC), and polypropylene. In all cases, at least
94% of the virions partitioned into the liquid fraction. In real sludge,
no more than 0.8% of virions partitioned to the solids and no more
than 6% to the material surface. Both MS2 and Phi6 partitioned more
to the surface of concrete and polypropylene than to the surface of
porcelain or PVC. Partitioning of viruses in wastewater among the
liquid, biosolids, and material surface does not appear to mitigate
the potential for aerosolization of virus, as most of the virus remains
in the liquid phase.