The Preparations Used to Study Calcium in Lens Epithelial Cells and Its Role in Cataract Formation

Andjelić, Sofija

doi:10.4172/2155-9570.s1-002

Cited by 5 publications

(7 citation statements)

References 6 publications

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“…12,13 The binding of ACh to M 1 receptors triggers the release of Ca 2+ from intracellular Ca 2+ stores. In a recent review of the publications related to the lens epithelium and calcium studies in the lens epithelium, 14 we summarised the relevant studies but found none that involved gentian violet staining. In the work presented here, the [Ca 2+ ] i responses to ACh of both gentian violet-stained and non-stained lens capsules were compared to assess the viability of the lens epithelial cells as well as other possible effects of gentian violet on the human anterior lens epithelial cell function.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Gentian Violet on Human Anterior Lens Epithelial Cells

Andjelić¹,

Zupančič²,

Hawlina³

2014

Current Eye Research

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The staining of the anterior lens capsule with gentian violet during phacoemulsification in concentration of 0.01%, does not have detectable cytotoxic effects, which would affect the [Ca(2+)]i homeostasis in lens epithelial cells. The data, if extrapolated to corneal endothelium, exposed to the same concentration, suggest that gentian violet in concentration of 0.01% is safe as an adjunct for capsule visualization in cataract surgery.

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Section: Introductionmentioning

confidence: 99%

The Effect of Gentian Violet on Human Anterior Lens Epithelial Cells

Andjelić¹,

Zupančič²,

Hawlina³

2014

Current Eye Research

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“…We have previously reviewed structural organization of the healthy lens epithelium (Andjelic et al. ), and we provided detailed evidence about the structural organization of the aLECs attachment to the BL in the healthy lens (Andjelic et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…We have previously reviewed structural organization of the healthy lens epithelium (Andjelic et al 2011), and we provided detailed evidence about the structural organization of the aLECs attachment to the BL in the healthy lens (Andjelic et al 2015a). The diffusion of water in human eye lenses is highly anisotropic and spatially inhomogeneous (Moffat & Pope 2002).…”

Section: Discussionmentioning

confidence: 99%

Anterior lens epithelium in cataract patients with retinitis pigmentosa – scanning and transmission electron microscopy study

Andjelić

Drašlar

Hvala

et al. 2016

Acta Ophthalmologica

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“…The LECs’ intercellular connections and the way of attachments to adjacent cells have been reviewed in Andjelic et al. (). They are involved in LEC contractions as well (Andjelic et al.…”

Section: Discussionmentioning

confidence: 99%

“…We show in detail their appearance on the LECs forming the confluent monolayer by SEM. The LECs' intercellular connections and the way of attachments to adjacent cells have been reviewed in Andjelic et al (2011a). They are involved in LEC contractions as well (Andjelic et al 2011b).…”

Section: Discussionmentioning

confidence: 99%

Morphological and proliferative studies on ex vivo cultured human anterior lens epithelial cells – relevance to capsular opacification

Andjelić

Drašlar

Lumi

et al. 2015

Acta Ophthalmologica

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ABSTRACT.Purpose: To determine the structural characteristics of lens epithelial cells (LECs) found on the anterior portion of the lens capsule and their pluripotency, proliferating and migrating potential when grown ex vivo with relevance to posterior capsular opacification (PCO) after cataract surgery. Methods: The explants of anterior portion of the lens capsule consisting of monolayer of LECs were obtained from uneventful cataract surgery and were cultivated under adherent conditions. The size and shape of the outgrowing cells were recorded by scanning electron microscopy (SEM), while their migration and proliferation potential were followed using light microscopy. Positivity for proliferation (Ki-67)-and pluripotency (Sox2)-specific markers were tested by immunofluorescent staining. Results: The proliferation and migration of anterior portion of the lens capsule's LECs filling up the denuded and reverse side regions of the lens capsule as well as their growth on glass culture surfaces could be followed by light microscopy and SEM, while the distribution of LECs and their morphology could be analysed in detail by SEM. The expression of Ki-67 and Sox2 in LECs growing adherently on human anterior portion of the lens capsule could also be detected. Conclusions: Classic light microscopy and SEM can be used to show that human anterior portion of the lens capsule harbours LECs that can proliferate and migrate, suggesting their pluripotency or putative stem cell nature. Similarly, morphological techniques can be used to study PCO and the effect different drugs or physical treatments have against PCO development.

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The Preparations Used to Study Calcium in Lens Epithelial Cells and Its Role in Cataract Formation

Cited by 5 publications

References 6 publications

The Effect of Gentian Violet on Human Anterior Lens Epithelial Cells

The Effect of Gentian Violet on Human Anterior Lens Epithelial Cells

Anterior lens epithelium in cataract patients with retinitis pigmentosa – scanning and transmission electron microscopy study

Morphological and proliferative studies on ex vivo cultured human anterior lens epithelial cells – relevance to capsular opacification

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