The presence of a secretory phospholipase A2 in the nuclei of neuronal and glial cells of rat brain cortex

Nardicchi, Vincenza; Macchioni, Lara; Ferrini, M.; Goracci, G

doi:10.1016/j.bbalip.2007.08.007

Cited by 17 publications

(12 citation statements)

References 54 publications

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“…Immunofluorescence analysis revealed that GV was highly localized in the nuclei of PC12 and U251 neuroblastoma cells [9]. This finding was confirmed by assaying the activity in neuronal and glial nuclei purified from rat brain, and subsequently identified by Western blot analysis [48]. Immunofluorescence analysis of isolated rat brain nuclei revealed that the enzyme was mainly localized in the nucleoplasm.…”

Section: Gvmentioning

confidence: 89%

“…However, the presence of sPLA 2 in intracellular compartments of neural cells [9,48] suggests that endogenous enzymes might also participate in apoptotic cell death. Indeed, inhibition of GIIA by a cell permeable specific inhibitor could reduce glutamate-induced death of cerebellar granule neurons [105].…”

Section: Involvement Of Spla 2 In Neurological Diseasesmentioning

confidence: 99%

“…The beneficial effect of this treatment was attributed to inhibition of GIIA because indoxam almost completely blocked the death of rat cultured neurons induced by exposure to recombinant human GIIA. However, indoxam is not a specific inhibitor of this isoform because it also reduces the activities of other isoforms including GV present in neuronal and glial nuclei [48].…”

Section: Indole Derivativesmentioning

confidence: 99%

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Low Molecular Weight Phospholipases A2 in Mammalian Brain and Neural Cells: Roles in Functions and Dysfunctions

2010

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Several "low molecular weight" or "secretory" phospholipases A(2) isoforms may be expressed in mammalian neural cells. Indeed, mRNAs for GIB, GIIA, GIIE, GIII, GV, GX, and GXII were detected in brain tissues despite different levels. However, only the presence of GIB, GIIA, and GV proteins has been clearly demonstrated in neural cells or in the nervous tissue. Although the roles of GIB and GV in the nervous tissue are still elusive, there is evidence to support the involvement of GIIA in physiological and pathological events, including neurotransmission, long-term potentiation, and neuritogenesis. The neurotoxic effects of an increase in GIIA may be envisaged under pathological conditions associated with the activation of astrocytes during inflammation or through activation of neurons and enzymes due to the stimulation of the NMDA glutamate receptor. In the past, elevation of GIIA expression in many acute and chronic neurological diseases is well known. Although each neurodegenerative disease has a separate etiology, many share similar neurochemical common processes, such as excitotoxicity, oxidative stress, and mitochondrial dysfunction, phenomena where GIIA play an important role.

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Section: Gvmentioning

confidence: 89%

Section: Involvement Of Spla 2 In Neurological Diseasesmentioning

confidence: 99%

Section: Indole Derivativesmentioning

confidence: 99%

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Low Molecular Weight Phospholipases A2 in Mammalian Brain and Neural Cells: Roles in Functions and Dysfunctions

2010

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“…For evaluation of neurite length, the average length of neurites in cells treated with NGF alone was considered as 100%. P \ 0.05 vs. control not affect the subcellular localization of GV, another isoform that has been demonstrated to be associated with nuclei in PC12 cells and U251 glioma cells and in nuclei isolated from rat brain cortex [18,22]. GX, another endogenous sPLA 2 isoform, is also involved in neuritogenesis [9] but apparently by another mechanism.…”

Section: Discussionmentioning

confidence: 95%

“…NGF-treatment did not affect the sub-cellular localization of another sPLA 2 isoform, group V (GV), which is also constitutively expressed by PC12 and localizes to the cytoplasm and nuclei under proliferation conditions [18,22]. Indeed, after treatment with NGF under identical conditions (Fig.…”

Section: Effect Of Ngf On Subcellular Localization Of Secretory Phospmentioning

confidence: 90%

Effect of NGF on the Subcellular Localization of Group IIA Secretory Phospholipase A2 (GIIA) in PC12 Cells: Role in Neuritogenesis

et al. 2010

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Phospholipases A(2) (PLA(2)s) are involved in neuritogenesis but the identity of the isoforms(s) contributing to this process is still not defined. Several reports have focused on secretory PLA(2)s (sPLA(2)) as the administration of exogenous sPLA(2)s to PC12 neuronal cells stimulates neurite outgrowth. The present study demonstrates that the endogenous group IIA sPLA(2) (GIIA), constitutively expressed in mammalian neural cells, changes its subcellular localization when PC12 cells are induced to differentiate by NGF treatment. Indeed, confocal analysis showed a time-dependent accumulation of GIIA in growth cones and neurite tips. Under identical conditions the subcellular distribution of another isoform (GV) was unaffected by NGF. Contrary to GX, another sPLA(2) isoform expressed by PC12 cells, the contribution of GIIA to neuritogenesis does not require its release in the extracellular medium.

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Metabolism and Functions of Platelet-Activating Factor (PAF) in the Nervous Tissue

Goracci¹,

Balestrieri²,

Nardicchi³

2009

Handbook of Neurochemistry and Molecular Neurobiology

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The presence of a secretory phospholipase A2 in the nuclei of neuronal and glial cells of rat brain cortex

Cited by 17 publications

References 54 publications

Low Molecular Weight Phospholipases A2 in Mammalian Brain and Neural Cells: Roles in Functions and Dysfunctions

Low Molecular Weight Phospholipases A2 in Mammalian Brain and Neural Cells: Roles in Functions and Dysfunctions

Effect of NGF on the Subcellular Localization of Group IIA Secretory Phospholipase A2 (GIIA) in PC12 Cells: Role in Neuritogenesis

Metabolism and Functions of Platelet-Activating Factor (PAF) in the Nervous Tissue

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