The Presence of Autoantibodies to Cytoplasmic Rod and Ring Particles in the Serum of Patients with Chronic Hepatitis C Virus Infection

Afsharzadeh, Fatemeh; Bokharaei‐Salim, Farah; Esghaei, Maryam; Monavari, Seyed Hamidreza; Merat, Shahin; Haj-Sheykholeslami, Arghavan; Keyvani, Hossein

doi:10.5812/hepatmon.42388

Cited by 5 publications

(6 citation statements)

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“…Detection of genomic HCV RNA was done by amplification of the 5′‐untranslated region (5′‐UTR) of HCV in plasma and PBMC specimens, RT‐nested polymerase chain reaction (RT‐nested PCR) method was used with special primers for this region . Genotyping was done by performing RT‐nested PCR and restriction fragment length polymorphism (RFLP) as previously described . Briefly, the PCR products of positive results for HCV infection were digested with four ( Scr FI, Hin fI, Mva I, and Bst UI) restriction enzymes (Fermentas GmbH), and then the undigested (173 bp [base pairs]) and digested PCR products of specimens, negative and positive controls and DNA size marker (50 bp) were visualized after electrophoresis on 3.0% agarose gel.…”

Section: Methodsmentioning

confidence: 99%

“…34 Genotyping was done by performing RT-nested PCR and restriction fragment length polymorphism (RFLP) as previously described. 31,35,36 Briefly, the PCR products of positive results for HCV infection were digested with four (ScrFI, HinfI, MvaI, and BstUI) restriction enzymes (Fermentas GmbH), and then the undigested (173 bp [base pairs]) and digested PCR products of specimens, negative and positive controls and DNA size marker (50 bp) were visualized after electrophoresis on 3.0% agarose gel. On the basis of the fragmentation pattern of digested PCR products, the genotypes of HCV were determined.…”

Section: Genomic Hcv Detection and Genotyping With Restriction Fragmentioning

confidence: 99%

“…On the basis of the fragmentation pattern of digested PCR products, the genotypes of HCV were determined. 35,36 2.6 | Sequencing of the NS5b region and 5′-UTR of the HCV The nonstructural protein 5B (NS5B) gene and the 5′-UTR were amplified using RT-nested PCR as described (with some modification). 37 were used in the nested-PCR assay were the same as previously described in detail.…”

Section: Genomic Hcv Detection and Genotyping With Restriction Fragmentioning

confidence: 99%

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Detection of HCV genome in peripheral blood mononuclear cells of Iranian seropositive and HCV RNA negative in plasma of patients with beta‐thalassemia major: Occult HCV infection

Kahyesh‐Esfandiary

Sadigh

Esghaei

et al. 2018

Journal of Medical Virology

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Beta (β) thalassemia major is a genetic blood disorder with a deficiency in the hemoglobin beta chain, requiring blood transfusion therapy. Multiple blood transfusions increase the risk of transmitting blood-borne infections. The aim of this study is to determine the frequency of hepatitis C virus (HCV) infection in Iranian individuals with β-thalassemia major. A total of 164 patients with β-thalassemia major were recruited for this study. HCV RNA testing was done on plasma and peripheral blood mononuclear cells (PBMCs) from the HCV seropositive samples (with reverse transcriptase-nested polymerase chain reaction [PCR] method using primers from the 5'-untranslated region [UTR]), and all HCV RNA positive samples were genotyped by the restriction fragment length polymorphism assay. For confirmation of the HCV genotyping in PBMCs of occult HCV infection [OCI]-positive patients, the PCR products of two different regions of HCV (5'-UTR and nonstructural protein 5B [NS5B]) were sequenced. Of 164 patients, 29.3% were positive for anti-HCV antibodies, and HCV RNA was detected in the plasma specimens of 13.4% patients and in the PBMC samples of 15.2% participants. The genomic HCV-RNA was detected in PBMC samples in 3 (6.3%) of the total 48 individuals who were HCV seropositive, and plasma HCV-RNA negative (occult HCV infection). The subtypes of HCV in the plasma and PBMC samples of three participants were not identical. This study shows that among this group of Iranian patients with β-thalassemia major, 13.4% had active HCV infection and 6.3% had occult HCV infection as evidenced by HCV RNA detected in PBMC specimens. Therefore, the design of a prospective study that focuses on the diagnosis of OCI can be very valuable and provide more information.

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Section: Methodsmentioning

confidence: 99%

Section: Genomic Hcv Detection and Genotyping With Restriction Fragmentioning

confidence: 99%

Section: Genomic Hcv Detection and Genotyping With Restriction Fragmentioning

confidence: 99%

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Detection of HCV genome in peripheral blood mononuclear cells of Iranian seropositive and HCV RNA negative in plasma of patients with beta‐thalassemia major: Occult HCV infection

Kahyesh‐Esfandiary

Sadigh

Esghaei

et al. 2018

Journal of Medical Virology

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“…On the other hand, Afsharzadeh et al. reported a single case with anti-RR positivity in a cohort of 120 HCV patients in Iran and this case also previously had received IFN/ribavirin antiviral therapy ( 22 ). Peker et al.…”

Section: Discussionmentioning

confidence: 99%

Retrospective evaluation of “Rods and Rings” pattern detected in the anti-nuclear antibody (ANA) indirect immunofluorescence (IIF) test

İnal,

Kurumanastırlı,

Taşkınoğlu

et al. 2024

Front. Immunol.

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IntroductionAnti-rods and rings (anti-RR) antibodies have recently been described as a cytoplasmic pattern in IIF-based screening of autoantibodies on HEp-2 cells and ICAP has named it as AC-23. It is most frequently related to drug-induced antibody generation. This study aimed to investigate the clinical significance of AC-23 positivity and its relevance to the diagnosis and/or follow-up of the associated diseases and/or drug use.MethodsA multicenter retrospective study was conducted among 10 hospitals from six different provinces in Türkiye from January 2017 to December 2021. The laboratory data and clinical information of 600 patients with positive anti-RR antibodies out of 547.558 HEp-2 IIF ANA samples were analyzed.ResultsThe distribution of AC-23 positive patients by year indicated a steady increase between 2017-2021. Anti-RR prevalence in post-COVID-19 period was significantly higher than that of pre-COVID-19 period (p=0.00). Concomitant ANA positivity was detected in 56.5% of patients, the most common patterns being AC-4 and AC-5 (41.1%). The most frequent pathology among the anti-RR positive patients was an autoimmune disease (19.83%); 28.57% of which had rheumatoid arthritis and 17.65% autoimmune liver disease. Among the 600 patients, 65 (10.83%) were diagnosed as hepatitis C virus (HCV) infection. Available data for 38 of the HCV patients revealed that 71.05% of them had a history of interferon alfa+ribavirin and 28.95% of them had a history of NS3/4/5A/5B polymerase inhibitor or protease inhibitor drug use. Significant increase in the rate of anti-RR positivity was observed in the post-COVID-19 period when compared to pre-COVID-19 period (p:0.00).DiscussionThis is the first multicenter study in Türkiye about the clinical association of anti-RR antibodies which may be ignored during routine HEp-2 IIF testing. Pathologies other than HCV should be taken into consideration in terms of the possible role of anti-RR in autoimmune diseases and other pathologies. The preliminary data obtained in this study suggest that anti-RR antibody development might also be associated to COVID-19, supporting the several previous data related to the potential of viruses triggering the formation of autoantibodies. Large-scale prospective studies should elucidate the clinical significance of RR pattern and determine its role in patient diagnosis and follow-up.

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“…Cytoplasmic RR autoantibodies identified by IIF on HEp-2 cell substrate occur with a high specificity in 0.8–5% of patients undergoing long-term antiviral double peg-IFN/RBV therapy [3,4]. There are also studies that identify their presence in up to 40% of patients that undergo treatment with IFN/RBV [5,6].…”

Section: Introductionmentioning

confidence: 99%

Long-term Antiviral Hepatitis C treatment associated with Rods and Ring Cytoplasmic antibodies

Feldrihan

Benea²,

Junie

2019

Medicine and Pharmacy Reports

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Aim. Clinical description of a patient diagnosed with chronic hepatitis C virus infection, which associated a rare anti-cytoplasmic pattern, known as "Rods and Ring".Method. Clinical case report.Results. A 76-year old female patient with chronic hepatitis C virus infection under treatment for several months with pegylated Interferon-Ribavirin (started eight months ago) presented for clinical and biological evaluation of the therapeutic response.Conclusion. This is the first reported clinical case of a patient with cytoplasmic filamentous rods and rings autoantibodies associated with chronic hepatis C from the Clinical Hospital IRGH Prof. Dr. O. Fodor Cluj-Napoca, Romania. The presence of these antibodies appears to be triggered by antiviral therapy. Although these are newly identified antibodies, they could be used as serological markers for detecting patients at risk of developing associated autoimmune pathologies or nonresponders to the antiviral therapy. Likewise, their detection could identify patients with occult hepatitis C infection.

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The Presence of Autoantibodies to Cytoplasmic Rod and Ring Particles in the Serum of Patients with Chronic Hepatitis C Virus Infection

Cited by 5 publications

References 32 publications

Detection of HCV genome in peripheral blood mononuclear cells of Iranian seropositive and HCV RNA negative in plasma of patients with beta‐thalassemia major: Occult HCV infection

Detection of HCV genome in peripheral blood mononuclear cells of Iranian seropositive and HCV RNA negative in plasma of patients with beta‐thalassemia major: Occult HCV infection

Retrospective evaluation of “Rods and Rings” pattern detected in the anti-nuclear antibody (ANA) indirect immunofluorescence (IIF) test

Long-term Antiviral Hepatitis C treatment associated with Rods and Ring Cytoplasmic antibodies

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