“…Those LDs might be actually located in cytoplasmic invaginations deep into the nucleus, but electron microscopic (EM) studies that followed demonstrated that LDs were present within the nucleoplasm. The EM studies revealed LDs in four situations: (i) the nucleolus of rat liver cells during thioacetamide-induced carcinogenesis (Thoenes, 1964), (ii) LDs in the nucleoplasm and in the lumen of the endoplasmic reticulum (ER)/nuclear envelope/NR in rat liver treated with carcinogenic N, N 0 -dimethyl-4-aminoazobenzene (Karasaki, 1969(Karasaki, , 1973, (iii) nuclear LDs in mouse liver treated with hepatotoxic polybrominated biphenyl (Martino et al, 1981), and (iv) nuclear LDs in mouse embryonic cells with lethal mutations (Hillman et al, 1970;Hillman and Hillman, 1975).…”