The presence of seminal plasma, especially derived from stallion semen, helps preserve chilled Asian elephant (<i>Elephas maximus</i>) sperm motility

Pinyopummin, Anuchai; Mahasawangkul, Sittidet; Kornkaewrat, Kornchai; Rattanapirom, S.; Leartsang, W.; Kitkha, Supatee

doi:10.1111/and.12690

Cited by 6 publications

(5 citation statements)

References 56 publications

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“…Restoring SP proportion with stallion (heterologous) SP gave better results for sperm motility and velocities than those of un‐restored and elephant SP restored samples. The beneficial effect of stallion SP on chilled elephant sperm has been previously reported (Pinyopummin et al., ). In this study, it was further demonstrated that there was a beneficial effect in highly diluted samples, which could be useful for an elephant AI procedure, in which a large volume of semen might be required for deposition in the female reproductive tract (Brown et al., ; Thongtip et al., ).…”

Section: Discussionmentioning

confidence: 64%

“…The component in stallion SP that supported elephant sperm motility was not identified. However, SP proteins might respond for this effect as previously discussed (Pinyopummin et al, 2017).…”

Section: Discussionmentioning

confidence: 81%

“…The effect of SP on diluted semen could vary depending on the source of SP and has been mentioned for bull (Garner et al., ), boar (Caballero et al., ) and ram (Domínguez et al., ). There was no effect of homologous SP, compared to autologous SP in chilled elephant sperm quality (Pinyopummin et al., ). Therefore, our investigation failed to show that an improvement in chilled semen quality by elephant SP supplementation might be due to the source of SP.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, obtaining optimal conditions for cooled storage of semen is important. In a previous report, the beneficial effect of SP for cooled storage of Asian elephant sperm was demonstrated (Pinyopummin et al, 2017). This study was conducted to further determine any dilution effect and the effect of restoring the proportion of SP in diluted semen, on sperm quality during cooled storage of Asian elephant semen.…”

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confidence: 95%

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Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Pinyopummin

Mahasawangkul²,

Nunklang

et al. 2018

Reprod Domestic Animals

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The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris-glucose-egg yolk extender (TE) was used for cooled storage at 4°C for 48 hr. In experiment 1 (n = 18), semen was diluted to 1:1, 1:3, 1:7 and 1:15 with TE (volume per volume). There were no significant changes in sperm viability and sperm with normal acrosome integrity among dilutions, but sperm motility and motility velocities were greater (p < .05) in the 1:1 dilution than those of the 1:7 and 1:15 dilutions at 48 hr of storage. In experiment 2, supplemented SP was derived from elephants and stallions. In experiment 2.1, diluted semen (1:7 dilution) was restored with SP to obtain a 1:2 proportion (n = 8). Sperm motility, viability and sperm with normal acrosome integrity were similar among treatments, but motility velocities were greater (p < .05) with stallion SP at 48 hr of storage. In experiment 2.2, diluted semen (1:15 dilution) was restored with SP to obtain a 1:3 proportion (n = 10). Sperm viability and sperm with normal acrosome integrity were similar among treatments at 48 hr of storage. However, sperm motility and motility velocities were greater (p < .05) with stallion SP than those of others. In conclusion, elephant sperm motility was affected by a dilution effect and restoration of SP proportion with stallion SP, but not with elephant SP, could improve motility in chilled highly diluted sperm.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 95%

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Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Pinyopummin

Mahasawangkul²,

Nunklang

et al. 2018

Reprod Domestic Animals

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“…Furthermore, seminal plasma derived from stallions can maintain the sperm motility of Asian elephants during the cooling process (Pinyopummin et al, 2017).…”

Section: Seminal Plasmamentioning

confidence: 99%

Relationship between insulin-like growth factor-1 and semen quality in Asian elephants (elephas maximus)

Yang¹

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Evidence has been increasingly suggested that insulin-like growth factor-1 (IGF-1) is related to steroidogenesis and spermatogenesis. This may be conducive to find the causes of poor sperm quality in Asian elephants (Elephas maximus). The present study aimed to investigate whether correlations among serum IGF-1 concentration, serum testosterone level and semen variables exist in elephants. A total of 17 ejaculates (1–3 ejaculates/bull) were collected by performing transrectal massage. Before each ejaculate, blood samples were obtained (n = 17). Subsequently, semen characteristics of each ejaculate were evaluated. Assessments of precision (calculations of the intra- and inter-assay coefficient of variation, CV) and accuracy (tests of linearity and spike-recovery) were performed for ELISA validation. An increase of serum IGF-1 concentration was found to correlate with the percentages of spermatozoa with intact acrosome (r = 0.53, P < 0.05) and normal head morphology (r = 0.48, P < 0.05). The serum IGF-1 concentration was positively correlated with serum testosterone level (r = 0.73, P = 0.004). The results of validation demonstrated the CV was 1.6–6.4% for intra-assay variability and 4.7–6.9% for inter-assay variability. Linearity under serial dilutions of a known serum concentration was confirmed (R2 = 0.99) with an acceptable recovery rate of each dilution (mean 114.7 ± 19%, ranging from 100 to 143%). Additionally, the mean percentage of recovery of spiked IGF-1 was 107.2 ± 21% (ranging from 80.8 to 136.9%). In summary, this commercial ELISA kit can be used to determine serum IGF-1 concentration in Asian elephants. Moreover, our findings suggest the increased IGF-1 may be relate to good sperm quality and that the relationship between serum IGF-1 and testosterone concentration indicates a crucial role in the fertility of male elephants. Further works are interesting to investigate the exact mechanism by which IGF-1 affects semen quality in elephants.

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Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma

Yang,

Suwimonteerabutr,

Angkawanish

et al. 2024

Theriogenology Wild

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The presence of seminal plasma, especially derived from stallion semen, helps preserve chilled Asian elephant (Elephas maximus) sperm motility

Cited by 6 publications

References 56 publications

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Relationship between insulin-like growth factor-1 and semen quality in Asian elephants (elephas maximus)

Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma

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