The presence of vacuoles in blastocysts is negatively associated with euploidy and live birth rates

Lee, Yu-Jen; Lin, Ying-Hsi; Cheng, En‐Hui; Chen, Chien‐Hong; Huang, Chun‐Chia; Lin, Pin‐Yao; Lee, Tsung-Hsien; Lee, Maw‐Sheng

doi:10.1016/j.fertnstert.2023.04.023

Cited by 2 publications

(3 citation statements)

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“…The presence of mononucleation in embryos and the proportion of mononucleated embryos to total embryos may be positively correlated with embryonic development potential and live birth rate [11][12] . The presence of vacuoles also affects the rate of aneuploidy and live birth of embryos [13] . But, multinucleation and vacuoles did not affect the formation of transferable blastocysts in this study, possibly because the frequency of multinucleation and vacuoles was too low to be prioritized for transplantation.…”

Section: Discussionmentioning

confidence: 99%

“…94 cases (47.24%) were primary infertility, and 52 cases (26.13%) underwent ICSI. The average number of retrieved oocytes was 13 (10)(11)(12)(13)(14)(15)(16), with a blastocyst formation rate of 41.46%(512/1235) and a high-quality blastocyst formation rate of 13.36%(165/1235) (Table 1).…”

Section: Basic Demographic and Clinical Characteristics Of Patientsmentioning

confidence: 99%

“…The presence of mononucleated blastomeres and the proportion of mononucleated blastomeres to total blastomeres may be positively correlated with embryo development potential and live birth rates [11][12] . The presence of vacuoles also affects the rates of aneuploidy and live births [13] .…”

Section: Introductionmentioning

confidence: 99%

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A new day 4 embryo evaluation system to predict the formation of transferable blastocyst

Dai,

Geng,

Tao

et al. 2024

Preprint

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Purpose To explore a day 4 embryo evaluation system based on the potential for the embryo to develop into a transferable blastocyst. Methods We observed the outcome of blastocyst culture for 1235 embryos from 199 patients. We considered the developmental stage, fragmentation, whether it was in a stage-specific cleavage pattern, and normal fertilization to formulate scoring criteria, based on whether they developed into transferable blastocysts. The receiver operative characteristics(ROC) curve was used to compare the predictive abilities of four criteria for day 4 evaluation to develop into transferable blastocysts and high-quality blastocysts. Results The developmental stage, fragmentation, normal fertilization and stage-specific cleavage pattern had the greatest impact on the formation of transferable blastocysts. There were significant differences in the formation rates of transferable blastocysts and high-quality blastocysts among different embryo grades. The areas under ROC curves of the day 4 embryo evaluation criteria constructed in this study was the highest in predicting transferable blastocyst formation, relative to the other three evaluation criteria. Conclusion This study observed the presence of pseudo-compacted embryos similar to cleavage-stage embryos. The Day 4 embryo evaluation criteria constructed in this study can effectively predict the formation of transferable blastocysts.

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Section: Discussionmentioning

confidence: 99%

Section: Basic Demographic and Clinical Characteristics Of Patientsmentioning

confidence: 99%

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A new day 4 embryo evaluation system to predict the formation of transferable blastocyst

Dai,

Geng,

Tao

et al. 2024

Preprint

View full text Add to dashboard Cite

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A new day 4 embryo evaluation criteria to predict the formation of blastocyst

Dai,

Yasong,

Tao

et al. 2024

Preprint

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Background Currently, in vitro fertilization and embryo transfer (IVF-ET) typically involve transferring embryos on the third (D3) or fifth day (D5). However, a fresh cycle fourth day (D4) morula transfer offers a flexible and effective alternative. The compaction on the fourth day serves as a simple and reliable indicator to predict embryo implantation potential. The use of D4 transfer is gradually gaining popularity. Nevertheless, there is still a need for a comprehensive evaluation system for fourth day embryos.Thus, this study proposes to explore a day 4 embryo evaluation system based on the potential for the embryo to develop into a transferableblastocyst. Methods We observed the outcome of blastocyst culture for 1235 embryos from 199 patients. We considered the developmental stage, fragmentation, whether it was in a stage-specific cleavage pattern, and normal fertilization on the fourth day. The fourth day embryo evaluation system was proposed by comparing them with the effect on blastocyst formation rate. The rates of blastocyst formation with different embryo grades were compared. The receiver operative characteristics(ROC) curve was used to compare the predictive abilities of four criteria for day 4 evaluation to develop into transferableblastocysts and high-quality blastocysts. Results The developmental stage, fragmentation, normal fertilization and stage-specific cleavage pattern had the greatest impact on the formation of transferable blastocysts. There were significant differences in the formation rates of transferableblastocysts and high-quality blastocysts among different embryo grades. The areas under ROC curves of the day 4 embryo evaluation criteria constructed in this study was the highest in predicting formation of transferable or high-quality blastocyst, relative to the other three evaluation criteria. This study observed the presence of pseudo-compacted embryos similar to cleavage-stage embryos on the rate of blastocyst formation. Conclusion The Day 4 embryo evaluation criteria constructed in this study can effectively predict the ability to develop into a blastocyst.

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The presence of vacuoles in blastocysts is negatively associated with euploidy and live birth rates

Cited by 2 publications

References 28 publications

A new day 4 embryo evaluation system to predict the formation of transferable blastocyst

A new day 4 embryo evaluation system to predict the formation of transferable blastocyst

A new day 4 embryo evaluation criteria to predict the formation of blastocyst

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