The Preservation of Bacteria and Fungi on anhydrous Silica Gel: an Assessment of Survival over Four years

Trollope, D.R.

doi:10.1111/j.1365-2672.1975.tb00511.x

Cited by 23 publications

(10 citation statements)

References 25 publications

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“…In addition, the silica gel preservation method also has been used successfully to preserve several other fungi (i.e., Aspergillus niger, Fusarium oxysporum, Gliocladium sp., Mucor hiemalis, Penicillium sp., Saccharomyces cerevisiae, Sordaria fimicola, Thamnidium elegans and Verticillium spp.) as reported by Trollope (1975) and 17 species of Fusarium as indicated by Windels et al (1988Windels et al ( , 1993. In addition, silica gel has also been used to preserve the viability of spores of rust fungi, which cannot be grown on agar.…”

Section: Silica Gel Preservationmentioning

confidence: 98%

Culture collections, the new herbaria for fungal pathogens

et al. 2010

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This paper discusses the importance of culture collections in plant pathology and reviews the methods currently available to store cultures. The preservation and maintenance of plant pathogenic fungi in a viable yet stable state for long periods has always been important, because isolates of these fungi can serve as standards for identification of quarantine taxa. Such isolates are also important for testing disease resistance and for plant breeding programs. The increasing use of molecular sequences analysis in the systematics of plant pathogenic fungi has meant that maintaining fungi in culture collections has become essential. Herein we discuss trends in the identification of plant and post-harvest pathogens, using Aspergillus, Colletotrichum, Phyllosticta and Mycosphaerella and its anamorphs as examples. Herbarium specimens, although still a requirement of the Botanical Code when describing new species are, perhaps, less important in providing useful information when defining a pathogenic species. Many pathogen groups consist of complexes of species and morphology alone can no longer distinguish among species. However, ex-type living cultures are essential for identification and future species comparisons that incorporate the use of molecular techniques. As such, ex-type cultures of any new species of pathogen, or when new diseases are reported or studies involving pathogenic strains are published, cultures of the taxa studied should be deposited in widely available culture collections, preferably in at least three members of World Federation for Culture Collections. Methods for the storage of fungal cultures such as water preservation, freezing, mineral oil overlay, freeze drying and lyophilization are reviewed in this paper. The main objective of culture preservation is to maintain the vigor and genetic characteristics of a pure culture. Therefore, safe long-term preservation methods are required to ensure fungal survival and retention of any valuable characteristics. To minimize the risk of any morphological, physiological, or genetic changes, several different preservation conditions should be used whenever possible. The present review also describes a complete preservation methodology that can be used for plant pathogenic fungi.

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Section: Silica Gel Preservationmentioning

confidence: 98%

Culture collections, the new herbaria for fungal pathogens

et al. 2010

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“…anisopliae F84-1-1 (Steinhaus and Marsh, 1962) was isolated and firstpassage spores preserved on silica gel (Trollope, 1975). This isolate was denoted strain 5 and was the parent from which all mutants were obtained.…”

Section: Fungal Strainsmentioning

confidence: 99%

HAPLOIDIZATION ANALYSIS OF HETEROZYGOUS DIPLOIDS OF THE ENTOMOGENOUS FUNGUSMETARHIZIUM ANISOPLIAE

Bergeron¹,

Messing-Al-Aidroos²

1982

Can. J. Genet. Cytol.

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All stages of the parasexual cycle required for genetic analysis by haploidization have been demonstrated for Metarhizium anisopliae Metsch. (Sor). Heterokaryons have been synthesized from strains carrying complementing markers, and heterozygotic diploid colonies have been isolated from the heterokaryons. After exposure to benlate or botran, diploid colonies gave rise to a large number of haploid segregants. Analysis of the segregation of eight mutations in three crosses assigned these genes to five possible linkage groups. Use of the parasexual cycle should facilitate genetic analysis of pathogenesis and enhance the usefulness of this fungus for the control of insect pests.

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“…Successful preservation of powdery mildew conidia appears to be influenced by their moisture content (Hermansen, 1972). Keeping in mind this observation, we started initially evaluating the suitability of silica gel as desiccating agent to preserve P. fusca conidia at −80°C, as silica gel is considered one of the methods for preservation of spore forming microorganisms (Trollope, 1975). Three isolates of P. fusca were used in this first approach.…”

Section: Resultsmentioning

confidence: 99%

Long‐term Preservation of Podosphaera fusca Using Silica Gel

Pérez-Garcı́a¹,

Mingorance²,

Rivera³

et al. 2006

Journal of Phytopathology

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Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain and one of the most important limiting factors for cucurbit production worldwide. As an obligate biotrophic parasite, this fungus has been traditionally cultured and conserved by periodical transfers of conidia to fresh plant material. Here we describe a simple protocol for preservation of P. fusca isolates in absence of living tissue based on the dry spore, slow-freezing technique, and demonstrate that storage of silica gel desiccated conidia at )80°C is an efficient method for the long-term preservation of the pathogen.www.blackwell-synergy.com

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The Preservation of Bacteria and Fungi on anhydrous Silica Gel: an Assessment of Survival over Four years

Cited by 23 publications

References 25 publications

Culture collections, the new herbaria for fungal pathogens

Culture collections, the new herbaria for fungal pathogens

HAPLOIDIZATION ANALYSIS OF HETEROZYGOUS DIPLOIDS OF THE ENTOMOGENOUS FUNGUSMETARHIZIUM ANISOPLIAE

Long‐term Preservation of Podosphaera fusca Using Silica Gel

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