The Preterm Rabbit: A Model for the Study of Acute and Chronic Effects of Premature Birth

Lorenzo, A.

doi:10.1203/00006450-198502000-00011

Cited by 23 publications

(25 citation statements)

References 13 publications

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“…The adult rabbit has been used extensively as a model for human adult thrombotic and hemorrhagic diseases and the rabbit pup has been used as a model for prematurity (26). The studies reported herein expand on the previous information on the rabbit pup coagulation system (2,27) and suggest that the rabbit pup range of levels for these factors is seen between the species.…”

Section: Resultssupporting

confidence: 49%

A Comparative Study of Coagulation Systems in Newborn Animals

1986

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ABSTRACf. Appropriate animal experimentation can enhance our understanding of thrombotic and hemorrhagic problems in the human neonate. Which newborn animal species' coagulation system most closely resembles the human neonate is unknown. The objective of the study was to assess the newborn coagulation system in four animal species and compare them with the human neonate. Blood samples were drawn on days 1 and 7 of life from lambs (n =10), piglets, (n =12), rabbit pups (n =12), and beagle pups (n = 7). Coagulation screening tests, specific factor assays, and specific inhibitors of the coagulation system were measured. All factor assays were expressed as a percent of the respective species pooled adult plasma. The results from the animals were compared to normal values from our laboratory for healthy full-term infants. The coagulation systems of all species tested, except the rabbit pup, were immmature at birth with most factor levels being lower than the adult of .their species. The coagulation systems were influenced by the postnatal age of the animal and the factor levels reached adult values in fewer days relative to the human. The coagulation system for the piglet most closely approximated the human neonate. The shared characteristics were prolonged screening tests, increased factor VIII:C, generally low levels for the contact and vitamin K-dependent factors, and low antithrombin III levels relative to the adult. The beagle pup also showed many similar characteristics but in contrast to the human neonate factor VIII:C and V were low on day 1 of life and prekallikrein was not measurable in the adult or newborn beagle. These data, especially the low antithrombin III levels, support the use of the piglet to investigate thrombotic problems in the neonate. The sick newborn infant is at risk of developing thrombotic and hemorrhagic problems in the postnatal period. The investigation of thrombotic and hemorrhagic problems in the neonate is often limited by the small size of the infant and the limited forms of investigation that are ethically acceptable in this group of patients. Despite the potential of animal models in investigating hemorrhagic and thrombotic problems in the neonate, there is limited information on the coagulation systems in newborn animals (1,2). The objective of this study was to assess systematically the coagulation system in four newborn animal species and compare them with the human infant in order to determine which animal(s) would be most appropriate for investigation of hemorrhagic and thrombotic problems in the human neonate. MATERIALS AND METHODSStudy population. Newborn lambs (mixed breed), piglets (mixed breed), rabbit pups (New Zealand White), and purebred beagle pups were studied on days I and 7 oflife. An adult plasma pool was prepared for each species from 20 adult animals of both sexes. Blood was collected directly into 0.13 M sodium citrate (1 part citrate to 9 parts blood except for the piglet for which the ratio was 1.35 citrate to 9 parts blood) from each animal by peripheral v...

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Section: Resultssupporting

confidence: 49%

A Comparative Study of Coagulation Systems in Newborn Animals

1986

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“…Although many species of rodents, nonhuman primates, and other laboratory animals develop acute hyaline membrane disease (HMD) (16)(17)(18)(19)(20)(21)(22) and even chronic interstitial lung disease similar to BPD (23)(24)(25), preterm baboons comprise the best nonhuman animal model for both HMD and its chronic sequelae, which appears clinically and pathologically most similar to human BPD (26)(27)(28)(29)(30)(31). The increasing incidence of BPD as one of the major unsolved problems in pediatric medicine resulted in the initiation of a collaborative program by the Na- PRN and requiring exogenous surfactant to survive, which is much more similar to the version of BPD currently seen in human infants (32).…”

Section: Introductionmentioning

confidence: 99%

Bombesin-like peptide mediates lung injury in a baboon model of bronchopulmonary dysplasia.

et al. 1998

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“…Since gestation takes 31 days in rabbits, and because 28 days gestational age is the limit of viability for these animals, 13 it is possible to analyze the effects of nutritional restriction and hyperoxia on lungs in the saccular phase of development, prior to alveolarization. 15 The diet employed had a lower protein content than rabbits milk has.…”

Section: The Current Model Was Chosen To Make It Possible Tomentioning

confidence: 99%

“…13 Nutritional restriction was defined as a 30% reduction in all nutrients in the control diet, based on experimental models of undernutrition. 18 The ponderal evolution of rabbits in this study was the same way of the study described on literature, that used the formula 13 All animals were fed twice a day by orogastric tube 4 Fr coupled to a scaled syringe. The following volumes were given: D0, 50 ml/kg/day; D1, 100 ml/kg/day; D2, 150 ml/kg/day; D3 to D10, 200 ml/kg/day.…”

Section: Preparation Of Animalsmentioning

confidence: 99%

“…12 An experimental model with preterm rabbits has been developed to study acute lung injury by hyperoxia and lung development. [13][14][15] However, few experimental models of nutritional restriction and hyperoxia with premature animals have been employed, 16 and, when they have been used, the impact on pulmonary architecture has not been analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Respostas pulmonares à restrição nutricional e à hiperoxia em coelhos prematuros

Mataloun¹,

Rebello²,

Mascaretti³

et al. 2006

J. Pediatr. (Rio J.)

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Objectives: To analyze the effects of nutritional restriction and hyperoxia on lung weight and pulmonary morphometry in premature rabbits during the first 11 days of life.Methods: New Zealand White rabbits were delivered by C-section at 28 days gestational age and randomized into four groups: control diet and room air, control diet and hyperoxia (> 95% O 2 ), nutritional restriction and room air and nutritional restriction and hyperoxia (> 95% O 2 ). Nutritional restriction was achieved by reducing all nutrients by 30% in comparison with the control diet. Lung tissue slides were stained with hematoxylin-eosin, modified resorcin-orcein and picrosirius, before morphometric analysis was performed.Results: From the fourth day onwards, less weight was gained by the nutritional restriction and hyperoxia group (p < 0.001) and from the sixth day on, by the nutritional restriction and room air group (p < 0.001), in comparison with their respective control groups. Nutritional restriction decreased alveoli number (p < 0.001) and collagen deposition (p < 0.001). Hyperoxia was responsible for reductions in number of alveoli (p < 0.001) and collagen deposition (p < 0.001), in addition to higher mean linear intercept values (p < 0.05) and thickening of alveolar septa (p < 0.001). When nutritional restriction was associated with hyperoxia, the reductions in number of alveoli (p < 0.001) and of collagen deposition (p < 0.001) intensified.Conclusions: Nutritional restriction intensified the changes of pulmonary architecture findings caused by hyperoxia, in particular through alterations to alveolarization and collagen deposition.J Pediatr (Rio J). 2006;82(3):179-85: Nutritional restriction, hyperoxia, lung development, premature rabbits.

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The Preterm Rabbit: A Model for the Study of Acute and Chronic Effects of Premature Birth

Cited by 23 publications

References 13 publications

A Comparative Study of Coagulation Systems in Newborn Animals

A Comparative Study of Coagulation Systems in Newborn Animals

Bombesin-like peptide mediates lung injury in a baboon model of bronchopulmonary dysplasia.

Respostas pulmonares à restrição nutricional e à hiperoxia em coelhos prematuros

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