Background. Hypertension is the leading cause of cardiovascular disease and premature death worldwide. Bank workers are at higher risk of hypertension because of their work sedentary characteristics. However, little is known about the prevalence and determinants of hypertension among this group of population. Therefore, this study aimed to assess the prevalence and associated factors of hypertension among bank employees in metropolitan cities in Amhara Regional State of Ethiopia. Method. An institution-based cross-sectional study was conducted among 368 bank employees. A simple random sampling technique was used to select participants. A pretested self-administered questionnaire and biophysical measurements were employed to collect the data. Descriptive statistics and logistic regression analyses were done to summarize the data and identify factors associated with hypertension, respectively. Result. The overall prevalence of hypertension among bank employees was 52.4% (95% CI: 47.2, 57.7). Increased age (AOR = 1.1, 95% CI: 1.03, 1.11), male sex (AOR = 2.5, 95% CI: 1.2, 5.1), overweight (AOR = 2.7, 95% CI: 1.5, 5.2), obesity (AOR = 5.6, 95% CI: 2.0, 11.3), moderate/high physical activity (AOR = 0.36, 95% CI: 0.2, 0.62), daily fruit intake (AOR = 0.1, 95% CI: 0.04, 0.3), stressful life event experience (AOR = 1.8, 95% CI: 1.01, 3.4), family history of hypertension (AOR = 2.8, 95% CI: 1.5, 5.4), and poor knowledge of CVDs (AOR = 2.4, 95% CI: 1.2, 4.8) were significantly associated with hypertension. Conclusion. The prevalence of hypertension among bank workers was very high. Increased age, male sex, overweight and obesity, daily fruit intake, moderate/high physical activity, the experience of stressful events, familial history of hypertension, and poor CVDs knowledge were associated with hypertension. Thus, raising awareness about cardiovascular disorders and behavior change interventions that enhance bank workers’ engagement in physical exercise, screening behavior, and a healthy diet is urgently required for this group of population.