The prevalence and distribution of Burkholderia pseudomallei in rice paddy within Hainan, China

Dong, Sufang; Wu, Lixian; Long, Fu‐Quan; Qiang, Wanmin; Liu, Xiang; Peng, Hua; Xu, Ke; Lü, Yajun; Wang, Ying; Lin, Yuan; Xia, Qianfeng

doi:10.1016/j.actatropica.2018.08.007

Cited by 22 publications

(20 citation statements)

References 29 publications

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“…pseudomallei , confirming a diagnosis of chronic melioidosis. Multilocus-sequence typing (MLST) of seven housekeeping genes, as described previously [ 3 ], identified the strain as belonging to sequence type 55 (ST55), one of the dominant types in Hainan [ 4 ].…”

Section: Presentation Of Casementioning

confidence: 99%

Prolonged intermittent fever and massive splenomegaly in a miner working in the tropical jungle, China

Liu

Zong

2020

PLoS Negl Trop Dis

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Section: Presentation Of Casementioning

confidence: 99%

Prolonged intermittent fever and massive splenomegaly in a miner working in the tropical jungle, China

Liu

Zong

2020

PLoS Negl Trop Dis

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“…has been classified as a category B bioterrorism agent by the Centers for Disease Control and Prevention of USA [3]. In China, the epidemic areas of melioidosis are mainly in Hainan, Guangdong and Guangxi province [4, 5]. Isolates from Hainan alone exhibited highly genetic diversity when tested by multilocus sequence typing (MLST) [6].…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay

et al. 2019

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Melioidosis is a severe infectious disease caused by gram-negative, facultative intracellular pathogen Burkholderia pseudomallei ( B . pseudomallei ). Although cases are increasing reported from other parts of the world, it is an illness of tropical and subtropical climates primarily found in southeast Asia and northern Australia. Because of a 40% mortality rate, this life-threatening disease poses a public health risk in endemic area. Early detection of B . pseudomallei infection is vital for prognosis of a melioidosis patient. In this study, a novel isothermal recombinase polymerase amplification combined with lateral flow dipstick (LF-RPA) assay was established for rapid detection of B . pseudomallei . A set of primer-probe targeting orf 2 gene within the putative type III secretion system (T3SS) cluster genes was generated and parameters for the LF-RPA assay were optimized. Result can be easy visualized in 30 minutes with the limit of detection (LOD) as low as 20 femtogram (fg) (ca. 25.6 copies) of B . pseudomallei genomic DNA without a specific equipment. The assay is highly specific as no cross amplification was observed with Burkholderia mallei , members of the Burkholderia cepacia -complex and 35 non- B . pseudomallei bacteria species. Moreover, isolates from patients in Hainan ( N = 19), Guangdong ( N = 1), Guangxi ( N = 3) province of China as well as in Australia ( N = 3) and Thailand ( N = 1) were retrospectively confirmed by the newly developed method. LODs for B . pseudomallei -spiked soil and blood samples were 2.1×10 3 CFU/g and 4.2×10 3 CFU/ml respectively. The sensitivity of the LF-RPA assay was comparable to TaqMan Real-Time PCR (TaqMan PCR). In addition, the LF-RPA assay exhibited a better tolerance to inhibitors in blood than TaqMan PCR. Our results showed that the LF-RPA assay is an alternative to existing PCR-based methods for detection of B . pseudomallei with a potentiality of early accurate diagnosis of melioidosis at point of care or in-field use.

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“…Comparison of clinical and environmental isolates using a combination of two discriminant genotyping techniques, multilocus sequence typing and multiple locus variable number tandem repeat analysis (MLST and MLVA), suggested an environmental origin for some of the cases. These studies were of limited scale, and could not determine the geographic distribution of B. pseudomallei all over the island, but from August to December 2016, Dong et al conducted a more comprehensive environmental B. pseudomallei investigation, covering all 18 counties of Hainan Island [51]. Among 360 sampling sites, 48 (13.3%) were positive for the bacterium, and most of these were located in coastal counties (12/18), which corresponds with the distribution of clinical cases around the island.…”

Section: Environmental and Molecular Investigations Of B Pseudomamentioning

confidence: 99%

Endemic Melioidosis in Southern China: Past and Present

Zheng

Xia

et al. 2019

TropicalMed

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Melioidosis is a severe tropical infectious disease caused by the soil-dwelling bacterium Burkholderia pseudomallei, predominantly endemic to Southeast Asia and northern Australia. Between the 1970s and the 1990s, the presence of B. pseudomallei causing melioidosis in humans and other animals was demonstrated in four coastal provinces in southern China: Hainan, Guangdong, Guangxi, and Fujian, although indigenous cases were rare and the disease failed to raise concern amongst local and national health authorities. In recent years, there has been a rise in the number of melioidosis cases witnessed in the region, particularly in Hainan. Meanwhile, although China has established and maintained an effective communicable disease surveillance system, it has not yet been utilized for melioidosis. Thus, the overall incidence, social burden and epidemiological features of the disease in China remain unclear. In this context, we present a comprehensive overview of both historical and current information on melioidosis in Southern China, highlighting the re-emergence of the disease in Hainan. Surveillance and management strategies for melioidosis should be promoted in mainland China, and more research should be conducted to provide further insights into the present situation.

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The prevalence and distribution of Burkholderia pseudomallei in rice paddy within Hainan, China

Cited by 22 publications

References 29 publications

Prolonged intermittent fever and massive splenomegaly in a miner working in the tropical jungle, China

Prolonged intermittent fever and massive splenomegaly in a miner working in the tropical jungle, China

Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay

Endemic Melioidosis in Southern China: Past and Present

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