The prevalence and pathogenic differences of <i>Porphyromonas gingivalis fimA</i> genotypes in patients with aggressive periodontitis

Miura, Masashi; Hamachi, Toshihiro; Fujise, O.; Maeda, Katsumasa

doi:10.1111/j.1600-0765.2005.00779.x

Cited by 66 publications

(78 citation statements)

References 26 publications

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“…The results that we obtained with cultured isolates confirmed the results of clinical investigations that fimA genotypes II and fimA IV are predominant in chronic periodontitis-affected sites (2, 7). Other investigators (35,36,38) have confirmed that genotype II is the most prevalent genotype at diseased sites, with genotype Ib being the second most prevalent in patients with aggressive or chronic periodontitis. These observations and the results from a similar study with healthy subjects with a predominance of genotypes I, III, and V (3) could indicate a difference in the fimbrillin monomer and, therefore, a difference in the composition of the fimbriae that could be responsible for the variation in the pathogenic potential of the major fimbriae of P. gingivalis.…”

Section: Discussionmentioning

confidence: 89%

“…In patients with chronic marginal periodontitis, P. gingivalis organisms with fimA genotype II are significantly more prevalent than isolates with other genotypes (2,4,7,35,37,38). Studies concerning the pathogenic potentials of distinct fimA genotypes also indicate that genotype II organisms are more prevalent in patients with aggressive periodontitis (36). The relationship of periodontal health and specific fimA genotypes has also been investigated, and it was found that that fimA genotype I is the most prevalent among P. gingivalis-positive healthy adults, followed by genotype V (3).…”

mentioning

confidence: 87%

“…The fimA genotypes of the 82 isolates included in this study were determined by using the same specific fimA primer sets and similar experimental conditions used in a number of studies that have used the direct PCR technique with clinical material for investigation of the prevalence of the fimA genotypes of P. gingivalis from sites in patients with various periodontal conditions (2,3,7,35,36,38). The results that we obtained with cultured isolates confirmed the results of clinical investigations that fimA genotypes II and fimA IV are predominant in chronic periodontitis-affected sites (2, 7).…”

Section: Discussionmentioning

confidence: 99%

“…The fimA genotyping was performed with primer sets specific for the different genotypes of fimA (3,7,35,36,38). The primer sets were synthesized by Operon Biotechnologies GmbH (Cologne, Germany).…”

Section: Methodsmentioning

confidence: 99%

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fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis

et al. 2008

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Fimbriae are important virulence factors of pathogenic bacteria, facilitating their attachment to host and bacterial cells. In the periodontal pathogen Porphyromonas gingivalis, the fimA gene is classified into six types (genotypes I, Ib, II, III, IV, and V) on the basis of different nucleotide sequences, with fimA genotypes II and IV being prevalent in isolates from patients with periodontitis. The aims of this study were to examine the distribution of fimA genotypes in a collection of 82 P. gingivalis isolates from adult periodontitis patients of worldwide origin and to investigate the relationship between the fimA genotypes and the sequence types (STs), as determined by multilocus sequence typing (MLST), of the isolates. The fimA gene was amplified by PCR with primer sets specific for each genotype. The STs of all strains were assigned according to the MLST database for P. gingivalis (www.pubmlst.org/pgingivalis). The 82 strains showed extensive genetic diversity and were assigned to 69 STs. Only isolates with closely related STs harbored the same fimA genotype. Twenty-eight (34.1%) strains harbored fimA genotype II, while only the reference strain for fimA genotype V reacted with the primers specific for this genotype. Twenty-one isolates (25.6%) were positive by more than one of the fimA PCR assays; the most frequent combinations were genotypes I, Ib, and II (eight isolates) and genotypes I and II (four isolates). Sequencing of the fimA gene from selected isolates did not support the observed specific fimA genotype combinations, suggesting that the genotyping method used for the major fimbriae in P. gingivalis should be reevaluated.

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Section: Discussionmentioning

confidence: 89%

mentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis

et al. 2008

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“…Periodontal diseases and inflammation are associated with specific P. gingivalis virulence factors (Tatakis & Kumar, 2005), including fimbriae (Miura et al, 2005;Weinberg et al, 1997), lipopolysaccharides (LPS) (Wang et al, 2002) and gingipains (Kadowaki et al, 1994Pathirana et al, 2007a;Potempa et al, 2000;Yoneda et al, 1990). Gingipains are cysteine proteases produced by P. gingivalis (Kadowaki et al, 1994;Potempa et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Cleavage of protease-activated receptors on an immortalized oral epithelial cell line by Porphyromonas gingivalis gingipains

et al. 2009

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Porphyromonas gingivalis activates protease-activated receptors (PARs) on oral keratinocytes, resulting in downstream signalling for an innate immune response. Activation depends on P. gingivalis gingipains, but could be confounded by lipopolysaccharide signalling through Toll-like receptors. We therefore hypothesized that P. gingivalis cleaves oral keratinocyte PARs in an Arg-(Rgp) or Lys-(Kgp) gingipain-specific manner to upregulate pro-inflammatory cytokines. Immortalized human oral keratinocytes (TERT-2) were incubated with wild-type P. gingivalis (ATCC 33277) or strains from a panel of isogenic gingipain deletion mutants: Kgp-deficient (KDP 129); Rgp-deficient (KDP 133); or Kgp-and Rgp-deficient (KDP 136). After incubation with P. gingivalis, keratinocytes were probed with specific antibodies against the N-terminus of PAR-1 and PAR-2. Using flow cytometry and immunofluorescence, receptor cleavage was marked by loss of specific antibody binding to the respective PARs. TERT-2 cells constitutively expressed high levels of PAR-1 and PAR-2, and lower levels of PAR-3. P. gingivalis ATCC 33277 cleaved PAR-1 and PAR-2 in a dose-dependent manner, while the receptors were unaffected by the protease-negative double mutant (KDP 136) at all m.o.i. tested. The single Kgp-negative mutant preferentially cleaved PAR-1, whereas the Rgp-negative mutant cleaved PAR-2. Wild-type or Kgp-negative mutant cleavage of PAR-1 upregulated expression of IL-1a, IL-1b, IL-6 and TNF-a; the Rgp-negative mutant did not modulate these cytokines. Selective cleavage of PAR-1 on oral epithelial cells by P. gingivalis Rgp therefore upregulates expression of pro-inflammatory cytokines.

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Classification and diagnosis of aggressive periodontitis

Fine

Patil

Loos

2018

Journal of Periodontology

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Conflicting data resulted for several reasons; 1) the classification was too broad, 2) the disease (AgP) was not studied from its inception, at differing time points (temporal), and at different locations (topographic). New technologic advances coupled with a more delimiting definition of disease will allow for genetic, host and microbial factor analyses in an unbiased manner. As such we predict that progress can be made in identifying a robust group of genetic, host, and microbial risk-markers associated with periodontal disease that can improve diagnostic capability in disease associated with juveniles, adolescents, and post-adolescent individuals.

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The prevalence and pathogenic differences of Porphyromonas gingivalis fimA genotypes in patients with aggressive periodontitis

Cited by 66 publications

References 26 publications

fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis

fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis

Cleavage of protease-activated receptors on an immortalized oral epithelial cell line by Porphyromonas gingivalis gingipains

Classification and diagnosis of aggressive periodontitis

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