The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE

Atabay, H.I.; Aydın, Fuat; Houf, Kurt; Şahin, Mitat; Vandamme, Peter

doi:10.1016/s0168-1605(02)00163-0

Cited by 69 publications

(66 citation statements)

References 48 publications

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“…A. butzleri, A. cryaerophilus and A. nitrofigilis produced red colonies on the medium. Engberg et al (2000) and Atabay et al (2002) found that the membrane filtration method is superior to the isolation method without filtration as the percentage of positive samples increased after filtration. Van Driessche et al (2003) proposed Arcobacter selective isolation broth (ASIB) and Arcobacter selective isolation agar (ASIA) for selective isolation of Arcobacter from feces.…”

Section: Media For Isolation Of Arcobactersmentioning

confidence: 99%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

138

130

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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Section: Media For Isolation Of Arcobactersmentioning

confidence: 99%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

138

130

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“…Because of its isolation from many human disease cases, A. butzleri is considered to be the most important species of the genus. Some studies have reported the isolation and molecular analyses of arcobacters from miscellaneous sources in Turkey (Atabay et al, 2003(Atabay et al, , 2008Aydin et al, 2007;Ertas et al, 2010). However, in human medicine, no studies have been conducted on arcobacters, except for one case report (Kayman et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Emerging pathogen Arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters

Kayman

Abay

Hızlısoy

et al. 2012

Journal of Medical Microbiology

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The aims of this study were as follows: (i) to isolate Arcobacter spp. from the stool samples of patients with gastroenteritis; (ii) to identify them with molecular methods; (iii) to genotype them using enterobacterial repetitive intergenic consensus (ERIC)-PCR; and (iv) to determine their antibiotic susceptibilities. For the study, a total of 3287 diarrhoeal stool samples submitted to the Microbiology Laboratory of the Kayseri Training and Research Hospital, Kayseri, Turkey, between 2010 and 2011 were analysed. Campylobacter blood-free selective medium supplemented with cefoperazone, amphotericin B and teicoplanin was used for isolation. Medium inoculated with stool samples was incubated microaerobically at 37 6C for 72-96 h. Phenotypic tests, a genusspecific PCR and a multiplex PCR were used to identify the arcobacters, whilst ERIC-PCR was used for genotyping and the antibiotic susceptibilities of the isolates were detected by E-test. Arcobacter spp. were isolated from nine of the 3287 samples. These nine isolates were identified as Arcobacter butzleri and all showed different ERIC-PCR profiles. All nine isolates were resistant to ampicillin and susceptible to gentamicin, tetracycline, erythromycin and ciprofloxacin. As far as is known, this is the first study in which A. butzleri has been isolated from human acute gastrointestinal infections in Turkey. According to these results, it is recommended that, when investigating the aetiology of infections of the digestive system in humans, Arcobacter spp. be considered for inclusion. The results of this study should contribute to our knowledge related to A. butzleri infections in humans. INTRODUCTIONThe genus Arcobacter is a member of the family Campylobacteraceae. Currently, the genus Arcobacter has a total of 15 recognized species: Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, Arcobacter nitrofigilis, Arcobacter cibarius, Arcobacter halophilus, Arcobacter mytili, Arcobacter thereius, Arcobacter marinus, Arcobacter trophiarum, Arcobacter defluvii, Arcobacter molluscorum, Arcobacter ellisi, Arcobacter bivalviorum and Arcobacter venerupis Figueras et al., 2011; Levican et al., 2012). Three species, namely A. butzleri, A. cryaerophilus and A. skirrowii, have been associated with gastrointestinal infections (Burnens et al., 1992;Jiang et al., 2010;Vandamme et al., 1992a) and extra-intestinal invasive diseases (Lau et al., 2002;On et al., 1995;Yan et al., 2000). In addition, asymptomatic arcobacter carriage in type 2 diabetic patients has been reported (Fera et al., 2010). Because of its isolation from many human disease cases, A. butzleri is considered to be the most important species of the genus. Some studies have reported the isolation and molecular analyses of arcobacters from miscellaneous sources in Turkey (Atabay et al., 2003(Atabay et al., , 2008Aydin et al., 2007;Ertas et al., 2010). However, in human medicine, no studies have been conducted on arcobacters, except for one case report (Kayman et al., 2010).The current study was undertake...

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“…have also been isolated from poultry carcasses, beef, pork, and water (7,(25)(26)(27), but poultry meat is more frequently contaminated than red meat (27). Hence, foods of animal origin and water are regarded as the major sources of the transmission of Arcobacter to humans.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibilities of various Arcobacter species

Ünver¹,

Atabay²,

Şahin³

et al. 2013

Turk J Med Sci

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On 6 September 2009 (GMT 21:49) a moderate Mw5.4 earthquake sequence burst at the eastern border of Albania with the Former Yugoslav Republic of Macedonia (FYROM). Th e main shock was located ~6 km north of the epicentre of the 30 November 1967 Mw6.2 Dibra (or Debar) earthquake, which caused loss of life and considerable damage to buildings. We use broad band waveforms recorded by the Hellenic Unifi ed Seismic Network (HUSN), which receives real-time waveforms from the neighbouring networks, to compute focal mechanisms, obtain the slip model and derive the ShakeMap of the mainshock. Th e focal mechanisms of 18 of the stronger events of the sequence, obtained through time-domain moment tensor inversion, indicate that deformation is taken up by NNE−SSW-trending normal faults, in agreement with the ~E−W extension previously identifi ed within the Albanian orogen. Our results show that the 2009 main shock ruptured a roughly 9 km normal fault at a depth of 6 km, which strikes at 194° and dips west at ~45°. Th e slip of the main shock was confi ned to a single patch of ~9 km × 6 km, the average slip was 5 cm and the peak slip was 18 cm. Th e slip model was incorporated in a forward modelling scheme to simulate the ground motion distribution in the near fi eld. Th e ShakeMap thus obtained, based on the distribution of Peak Ground Velocity at phantom stations, outlines the mesoseismal area within the Dibra and Bulqiza districts in Albania, in accordance with macroseismic observations. Th e region aff ected by the 2009 sequence, together with the seismogenic region of the 1967 Dibra event, form a roughly NNE−SSW-trending structure which is an active seismotectonic zone in eastern Albania constituting a threat for nearby urban areas.

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The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE

Cited by 69 publications

References 48 publications

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Emerging pathogen Arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters

Antimicrobial susceptibilities of various Arcobacter species

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