The Prevalence of blaCTX-M, blaTEM Genes in  Bacteria Isolated From Bladder Cancer Patients with UTI

Abstract

Urinary tract infections (UTIs) are among the most prevalent infections in cancer patients . Two hundred urine samples were taken from patients with bladder cancer between the dates of 1 April 2021 and 15 October 2021 while they were enrolled in third floor Ghazi Al- Hariri Hospital, Medical City, Baghdad Province, and private clinics in Nasiriyah Province (100 from patients treated with MMC and 100 from patients treated with BCG). Forty urine samples were taken from patients with UTI without Bladder Ca (control). The most commonly pathogens were Escherichia coli , followed by Klebsiella pneumoniae , Pseudomonas aeruginosa and Staphyllococcus aureus and other genera of UTI bacterium. The total ESBLs Producers in the current study from 40 isolates was (27)72.5%.The highest ESBLs Producer in all Groups was E.coli followed by K.pneumoniae and P.aeruginosae . According to PCR out of 38 Clinical specimen from all groups , results of the bla CTX-M gene indicated its presence in 21 isolate(55.2%),while the presence of bla TEM gene was 26(68.4%) of total isolates.

Microbial Detection and Antibiotic Susceptibility patterns of Clinical Isolates from Women With Urinary Tract Infection in AL-Nasiriyah City/Iraq

“…In current study, the frequency of positive cultures for microorganisms isolated from urine samples (90%). The results in present study in accordance with previous studies in Ethiopia [26], and convergence with other studies [27] and [28].The finding is higher than reported conducted in Baghdad city, Iraq [29] and Italy [30]. The differences could be attributed to geographical variations where the studies conducted as well as sample size, sanitation status, educational level, awareness, community customs and sexual traditions [29].…”

“…However, their effectiveness relies on susceptibility of pathogenic bacteria. Therefore, understanding bacterial susceptibility patterns to antibiotics is critical for management of all types of bacterial infections [28]. In our study, both gram positive and gram-negative bacteria isolated were resistant to different classes of antibiotics.…”

“…The reason for the most prominent uro-pathogenic Escherichia coli due to their unique structures and number of virulence factors such as flagella, pili, which help attach to uroepithelium. Furthermore, microorganisms spreading from per urethral areas contaminated with fecal flora due to proximity to anus and warm, moist environment to the urethra and bladder lead to increase the risk infection [45]and [28].…”

“…One-hundred and fifty samples included of patients women and control group have been divided into four age groups in this study. The age group of females were (15)(16)(17)(18)(19)(20)(21)(22)(23)(24), (25)(26)(27)(28)(29)(30)(31)(32)(33)(34), (35)(36)(37)(38)(39)(40)(41)(42)(43)(44), and (above 44 years). The study recorded the majority of both patients and control group were in the age (25-34) second group 46.3% and 38.10% respectively.…”

Investigation of Antimicrobial Susceptibility Patterns and blaVIM -metallo-β-lactamase Gene in Clinical Samples of Klebsiella pneumoniae

“…In our study, it was found that Klebsiella pneumoniae was as an opportunistic multidrug-resistant bacteria(MDR). Some genes for the resistance enzymes bla CTX-M and bla TEM were detected in these bacteria by PCR [23].…”