The prevalence of brucellosis of farm animals using serum- and milk- ELISA test in Al- Najaf province

Abstract

A total of 412 milk and sera samples were collected from the same animals of 88 buffaloes, 212 cattle, 84 sheep and 28 goat were screened by indirect ELISA (i ELISA) milk confirmed with serum i- ELISA. The results of seropositive i-ELISA were 121(29.36%) from all farm animals and distributed to 20 (22.27%) buffaloes, 36 (16.98%) cattle , 56(66.6%) sheep and 9 (32.14%) goat . The positive results of milk i ELISA were 89 (21.6%) from all farm animals and distributed to 8 (9.09%) , 32 (15.09%) , 44(52.83%) and 5(17.85%) represent buffaloes , cattle , sheep and goat respectively . epidemiological informations of animals collected by questionary about ( abortion , stillbirth and previous history vaccination against brucellosis) . The prevalence was relatively higher in sheep and goat followed by buffaloes and cattle . The sera and milk i ELISA test is only the best serological tests and has a high efficiency and accuracy in the diagnosis and inexpensive and can be used to control of the disease.