The prevalence of CMY-2, OXA-48 and KPC-2 genes in clinical isolates of Klebsiella spp.

Tavakoly, Talayeh; Jamali, Samar; Mojtahedi, Ali; Mirzaei, Mohammadali Khan; Shenagari, Mohammad

doi:10.14715/cmb/2018.64.3.7

Cited by 17 publications

(10 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nowadays, quinolones are frequently used to treat UTIs. Multi-drug resistance microorganisms, particularly extended spectrum beta-lactamases (ESBLs), producing ones, increased in Gram-negative bacteria as a result of the misuse of antibiotics (14)(15)(16)(17). The present study aimed at investigating the frequency of quinolone resistance in E. coli strains isolated from patients referred to hospitals in the North of Iran.…”

Section: Discussionmentioning

confidence: 99%

A Survey of gyrA Target-Site Mutation and qnr Genes among Clinical Isolates of Escherichia coli in the North of Iran

Yousefi

Mojtahedi

Shenagari

2018

Jundishapur J Microbiol

Self Cite

View full text Add to dashboard Cite

Background: Urinary tract infection (UTIs) caused by Escherichia coli is one of the most common human complications. The discriminate use of antibiotics leads to increasing multiple-drug resistance (MDR) in E. coli. Objectives: This study aimed at investigating the frequency of fluoroquinolones resistance by detecting qnr genes and mutation in gyrA gene in clinical isolates of E. coli in the North of Iran. Methods: This cross-sectional study was conducted on 309 E. coli isolates collected from major hospitals in Rasht, North of Iran. All isolates were identified by standard microbiological methods. Antimicrobial susceptibility testing was conducted by the disk diffusion method for the three quinolone antibiotics. The detection of gyrA, qnrS, qnrB, and qnrA genes was performed by the polymerase chain reaction (PCR) method. PCR products of gyrA gene were digested by HinfI enzyme to identify the mutation in gyrA gene by restriction fragment length polymorphism (RFLP) method. Results: Of 309 tested E. coli isolates, the most common resistance was observed against nalidixic acid (60.2%) and the least common one against norfloxacin (40.5%). The prevalence of qnrS, qnrB, and qnrA genes was 4.5%, 73.1%, and 62.8%, respectively. Mutation in gyrA gene was observed in codon 83 in 56.3% of the strains. Conclusions: In summary, despite the significant association of the investigated genes with fluoroquinolones resistance in these bacteria, the presence of these genes in susceptible strains suggested that some other possible mechanisms may also influence resistance against fluoroquinolones.

show abstract

Section: Discussionmentioning

confidence: 99%

A Survey of gyrA Target-Site Mutation and qnr Genes among Clinical Isolates of Escherichia coli in the North of Iran

Yousefi

Mojtahedi

Shenagari

2018

Jundishapur J Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…pneumoniae is responsible for nosocomial outbreaks worldwide and because of spread rapidly in the hospital, associated with high morbidity and mortality (17,18). The important concern that must be considered in the management of nosocomial infections caused by K. pneumoniae are periodic surveillance to identify the ESBL-producing strains for optimizing available infection control policies and treatment options in different parts of the hospital (9,17,19).…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of ESBL-producing Klebsiella pneumoniae isolates in intensive care units of a tertiary care hospital, North of Iran

Ghaffarian

Hedayati

Ebrahim‐Saraie

et al. 2018

Cell Mol Biol (Noisy-le-grand)

Self Cite

View full text Add to dashboard Cite

The emergence of extended-spectrum beta-lactamases (ESBL) producing strains become a great concern, because few antimicrobial agents remain active against them. Due to the lack of data on the genotyping characteristics and antibiotics resistance of clinical isolates of Klebsiella pneumoniae in the north of Iran, this study aimed to determine the occurrence of ESBL-producing isolates and their molecular characteristics in order to analyses their epidemiological relationships. This cross-sectional study performed on 60 K. pneumoniae isolates which were recovered from different clinical specimens within May and November 2016. Isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Antimicrobial susceptibility testing was carried out by disk diffusion method. The genetic relatedness among the isolates was assessed by RAPD-PCR. Totally, the lowest level of susceptibility was toward amoxicillin/clavulanat, and nalidixic acid. On the other hand, the highest level of susceptibility was toward imipenem (86.7%). The rate of ESBL-producing isolates was 45% (27/60). There was a significant association between production of ESBLs and higher antibiotic resistance in tested isolates. The RAPD-PCR dendrogram revealed 5 major clusters with a similarity of 80% which indicates the high relatedness of the studied isolates. Twenty-one isolates out of the 27 ESBL-producing isolates were clustered in cluster A. In summary, results showed the high prevalence of multiple-drug resistant and ESBL-producing K. pneumoniae isolates in our ICUs. Also, results revealed a significant similarity between ESBL-producing isolates that necessitate restricted infection control policies and rational prescription and use of antibiotics.

show abstract

“…In this study, we were able to isolate various bacterial and fungal species, cultured from Bodkin tubes, retrieved following DCR surgery. Given the widespread of resistant strains in country [1,[23][24][25], the other objective of the study was to perform a comprehensive evaluation of the antimicrobial resistance patterns among our samples.…”

Section: Discussionmentioning

confidence: 99%

Microbial Etiology and Antibacterial Resistance Patterns of Dacryocystorhinostomy Cases in the North of Iran S

Nemati

Mojtahedi

Montazeri

et al. 2018

Asian J Pharm Clin Res

Self Cite

View full text Add to dashboard Cite

Objective: Nasolacrimal duct obstruction is an annoying medical condition in both ophthalmology and otolaryngology practices. As prophylactic antibiotic therapy decreases the rates of infections, understanding the prompt microbial spectrum of the pathogens has been revealed to be highly effective in such patients. We evaluated the microbial growth and bacterial resistance patterns of Bodkin tube samples obtained from patients who underwent dacryocystorhinostomy (DCR).Methods: A total number of 72 patients were enrolled in the study, and their Bodkin tube samples were cultured 8–12 weeks after DCR at Amir-Al Momenin University Hospital of Rasht, in the north of Iran.Results: Staphylococcus epidermidis and Aspergillus fumigatus were the most common bacterial and fungal species derived from the cultures, and 23.6% and 36.1% of the patients had neither bacterial nor fungal growth, respectively. The highest antibiotic resistance rates were toward cefazolin and cloxacillin among all the studied antibiotics.Conclusion: Majority of cases had positive bacterial or fungal growth in their samples. Considering microbial resistance, proper antibiotic selection is recommended for the patients undergoing DCR.

show abstract

The prevalence of CMY-2, OXA-48 and KPC-2 genes in clinical isolates of Klebsiella spp.

Cited by 17 publications

References 20 publications

A Survey of gyrA Target-Site Mutation and qnr Genes among Clinical Isolates of Escherichia coli in the North of Iran

A Survey of gyrA Target-Site Mutation and qnr Genes among Clinical Isolates of Escherichia coli in the North of Iran

Molecular epidemiology of ESBL-producing Klebsiella pneumoniae isolates in intensive care units of a tertiary care hospital, North of Iran

Microbial Etiology and Antibacterial Resistance Patterns of Dacryocystorhinostomy Cases in the North of Iran S

Contact Info

Product

Resources

About