The prevalence of genes encoding leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in Taleghani hospital, Ahvaz, Iran

Khosravi, Azar Dokht; Hajar, Hoveizavi; Farshadzadeh, Zahra

doi:10.1016/j.burns.2011.08.002

Cited by 61 publications

(53 citation statements)

References 27 publications

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“…This is contrary to the findings of Rodrigues et al who stated that none of pvl positive MRSA strains harbored SCCmec type IV (30). The findings of the present study along with those reported in other studies (19) show high prevalence of pvl gene among MSRA strains. These findings emphasize the importance of early diagnosis and treatment of infections caused by MRSA strains harboring pvl gene.…”

Section: Discussioncontrasting

confidence: 99%

“…The prevalence rate of MRSA in the present study was 94.5%. In agreement with our results, Abbasi-Montazeri et al (18), Namvar et al (10), and Khosravi et al (19) reported the rates of 88.6%, 72.7%, and 87.36% for MRSA in burn patients in Iran, respectively. The high prevalence rate of MRSA in burn patients was also reported in Korea (98%) (1), China (98%) (20), Iraqi Kurdistan (88%) (21), and India (78%) (22).…”

Section: Discussionsupporting

confidence: 93%

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Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Afshari

Sepahi

Goudarzi

et al. 2017

Arch Clin Infect Dis

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Background: Methicillin resistance Staphylococcus aureus (MRSA) isolates have been recognized as one of the most important causes of infection in burn patients that have recently shown a frequent and rapid development of antibiotic resistance. Objectives: The present study described the distribution of different SCCmec types and antibiotic resistance pattern among MRSA strains isolated from burn patients in a referral burn center in Tehran, Iran. Methods: During a 12-month study, 189 MRSA isolates were obtained from burn patients. Standard culture and biochemical tests were used for identification of MRSA isolates. The antibiotic susceptibility testing was performed using disc diffusion method and the presence of mecA, nucA, pvl, and tst encoding genes was determined using PCR method. In addition, the different SCCmec types were determined using multiplex PCR. Results: All the MRSA isolates were observed to be resistant to amoxicillin and penicillin and sensitive to linezolid, teicoplanin, and vancomycin. The rates of resistance to other antibiotics varied from 86.2% for amikacin to 17.5% for quinupristin-dalfopristin. MDR was observed in all the isolates. Six different antimicrobial resistance patterns were observed among our isolates. Based on the multiplex PCR assay, the two different SCCmec types were detected as 71.4% type III and 28.6% type IV. In total, 8.5% of isolates harbored pvl toxin encoding gene all of which belonging to SCCmec type IV. Furthermore, 33 isolates (17.5%) harbored tst encoding gene. Conclusions:The results showed low diversity of SCCmec type among circulating MRSA in the burn center with relatively high prevalence of SCCmec III. These findings support the need for more studies to elucidate distribution of different SCCmec types among MRSA isolated from burn patients.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 93%

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Afshari

Sepahi

Goudarzi

et al. 2017

Arch Clin Infect Dis

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“…Although pvl cannot be used as a sole marker of CA-MRSA, it is important to promptly diagnose and treat infections caused by S. aureus strains harboring the pvl gene. In the current study, the incidence rate of MRSA strains harboring the pvl gene was observed to be 21.4%, which is similar to the results of other studies that reported the prevalence to be 2% -35% among MRSA strains (30,31). Most of the isolates carrying the pvl gene were obtained from blood samples (66.7%), which is in contrast to the findings reported in other studies, where skin and soft tissue infections were the main presentations (30).…”

Section: Discussionsupporting

confidence: 84%

Distribution of Adhesion and Toxin Genes in Staphylococcus aureus Strains Recovered From Hospitalized Patients Admitted to the ICU

Eftekhar

Rezaee

Azad

et al. 2016

Arch Pediatr Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are a common cause of emerging nosocomial infections and are a major public health concern. Objectives: The aim of the present study was to determine the prevalence of MRSA, its antibiotic resistance pattern, and the virulence gene profiles in hospitalized patients admitted to ICUs. Methods: During a 6-month period, a total of 70 S. aureus isolates were collected from 249 patients admitted to the ICU in five hospitals. In vitro antibiotic susceptibility testing of the S. aureus isolates was carried out using the Kirby-Bauer disk diffusion method with 16 antibiotic disks. Molecular detection of toxin and adhesion genes was carried out using PCR. Results: All the 70 S. aureus isolates were confirmed to be MRSA strains. The largest number of S. aureus isolates was found in the blood (42.9%) and wound (21.4%) samples. The MDR pattern was detected in 71.4% of the isolates, which were obtained from wound and blood samples. Simultaneous resistance to seven, six, five, four and three drugs was common in 35 (50%), 7 (10%), 8 (11.4%), 11 (15.7%), 2 (2.9%) and 5 (7.1%) isolates, respectively. The frequency of the spa, fnbB, fnbA, clfB, clfA, can, bbp, ebp, etb, eta, pvl, and tst genes was 100%, 75.7%, 74.3%, 78.6%, 71.4%24.3%, 0%, 58.6%, 2.9%, 7.1%, 21.4%, and 51.4%, respectively. In addition, among all the examined genes, the clfB (78.6%) and etb (2.9%) genes had the highest and lowest prevalence respectively. Conclusions: In the present study, we found a high prevalence of MRSA at the hospitals studied. The findings emphasized the increased prevalence of MRSA isolates containing different toxin and adhesion genes, probably accompanied by antimicrobial resistance. Infection with such isolates worsens the clinical outcomes as well as the morbidity and mortality rates in hospitalized patients in ICUs.

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“…MRSA represents a significant cause of morbidity and mortality in both hospital and community settings, which raises serious concerns in the treatment of staphylococcal infections; hence, the accurate and early determination of methicillin resistance is a necessity for the prognosis of S. aureus infections (5). Most MRSA and some methicillin-susceptible S. aureus (MSSA) isolates produce Panton-Valentine leukocidin (PVL), a pore-forming toxin consisting of two subunits S (LukE) and F (LukD) (6,7). It has become increasingly important as a virulence factor in necrotizing SSTIs such as furuncle and pneumonia (6).…”

Section: Introductionmentioning

confidence: 99%

“…Most MRSA and some methicillin-susceptible S. aureus (MSSA) isolates produce Panton-Valentine leukocidin (PVL), a pore-forming toxin consisting of two subunits S (LukE) and F (LukD) (6,7). It has become increasingly important as a virulence factor in necrotizing SSTIs such as furuncle and pneumonia (6). Both MSSA and community-associated MRSA can express PVL (8).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and molecular characteristics of methicillin-resistantStaphylococcus aureus from skin and soft tissue infections in Shiraz, Iran

Mehrshad¹,

Haghkhah²,

Aghaei³

2017

Turk J Med Sci

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IntroductionAmong staphylococcal species, Staphylococcus aureus has been shown as a clinically relevant human pathogen, and in many individuals it may lead to asymptomatic colonization (1). Various infections from skin and soft tissue infections (SSTIs) to life-threatening infections like endovascular infections, pneumonia, septic arthritis, endocarditis, osteomyelitis, and sepsis are caused by this bacterium (2). Carriage of S. aureus is a risk factor for subsequent infection in various settings. Recent investigations suggest that nares-only screening may underestimate the prevalence of S. aureus colonization, and accurate determination requires sampling from other body sites (3). The spread of antibiotic resistance among strains of S. aureus has been dramatically elevated, such that methicillin-resistant S. aureus (MRSA) is recognized as a nosocomial pathogen worldwide. MRSA strains were once exclusively limited to hospital care; however, after outbreaks of infection in hospitals and health care facilities they have increasingly been detected among patients in the community who lack conventional risk factors for MRSA infection (4). MRSA represents a significant cause of morbidity and mortality in both hospital and community settings, which raises serious concerns in the treatment of staphylococcal infections; hence, the accurate and early determination of methicillin resistance is a necessity for the prognosis of S. aureus infections (5). Most MRSA and some methicillin-susceptible S. aureus (MSSA) isolates produce Panton-Valentine leukocidin (PVL), a pore-forming toxin consisting of two subunits S (LukE) and F (LukD) (6,7). It has become increasingly important as a virulence factor in necrotizing SSTIs such as furuncle and pneumonia (6). Both MSSA and community-associated MRSA can express PVL (8). Contrary to European countries (9), PVL-associated infections are increasing in some regions of Iran (10). This study aimed to assess the microbiological, epidemiological, and clinical features of purulent skin infections caused by S. aureus in Shiraz, southern Iran.Background/aim: Panton-Valentine leukocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) infections are increasing in some regions of Iran. The aim of the current study was to assess the epidemiology and molecular characteristics of S. aureus isolated from patients with skin infections in Shiraz, Iran.Materials and methods: Swab samples were obtained from patients admitted to the skin and burn units of hospitals. The medical records of each patient were collected via questionnaire. All staphylococcal isolates were collected and examined by conventional methods for detecting S. aureus strains. PCR was used to detect S. aureus harboring the mecA and pvl genes.Results: Out of 243 staphylococcal isolates, 55 (22.6%) S. aureus and 91 (37.4%) S. epidermidis were detected. Of the 45 patients, 21 (46.7%) were S. aureus carriers. The mecA gene was identified in 60% of S. aureus isolates, and the rest were sensitive to methicillin. Of the S. aur...

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The prevalence of genes encoding leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in Taleghani hospital, Ahvaz, Iran

Cited by 61 publications

References 27 publications

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Distribution of Adhesion and Toxin Genes in Staphylococcus aureus Strains Recovered From Hospitalized Patients Admitted to the ICU

Epidemiology and molecular characteristics of methicillin-resistantStaphylococcus aureus from skin and soft tissue infections in Shiraz, Iran

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