The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

Abstract

The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37 o C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14% ) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.

Antimicrobial susceptibility of Actinobacillus pleuropneumoniae strains isolated from pig's clinical samples in Serbia

“…A significant reduction of resistance for these two antimicrobial agents might have occurred as a result of their reduced usage during recent years, especially in treating animals from the category 15-40 kg of body weight. In several previous Serbian studies (27,33,35) it was found that the most common respiratory bacterial pathogens in pigs were Pasteurella multocida, A. pleuropneumoniae and Haemphilus parasuis. Generally, in the treatment of infections caused by these pathogens tetracycline and trimethoprim/sulfamethoxazole are less efficient in comparison to other antibiotics, so this may partly explain the reduced usage of these two drugs in veterinary practice in Serbia.…”