The Prevalence of ISAba1 and ISAba4 in Acinetobacter baumannii Species of Different International Clone Lineages Among Patients With Burning in Tehran, Iran

Bahador, Abbas; Raoofian, Reza; Farshadzadeh, Zahra; Beitollahi, Leyli; Khaledi, Azad; Rahimi, Sara; Mokhtaran, Masoumeh; Tavana, Ali Mehrabi; Esmaeili, Davood

doi:10.5812/jjm.17167v2

Cited by 27 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absence of OXA-58-like encoded genes in all the isolates in our study is consistent with the findings of similar studies (34,37,40). The important thing is that an ISAba1 element was detected in all the A. baumannii strains.…”

Section: Discussionsupporting

confidence: 92%

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Mohammadi

Goudarzi

Hashemi

et al. 2016

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

Background: The presence of carbapenemase-producing Acinetobacter baumannii has become a growing concern in patients who are hospitalized in burns centers. Objectives: The aims of this study were to determine the antimicrobial susceptibility patterns and prevalence of blaOXA carbapenemases, as well as to detect the presence of ISAba1, in A. baumannii strains carrying OXA genes obtained from burns patients at Shahid Motahari hospital, Tehran, Iran. Methods: From August 2013 to March 2014, 100 clinical A. baumannii isolates were collected from patients who were admitted to the burns ward at Shahid Motahari hospital. Antimicrobial susceptibility was determined using a disc diffusion test. PCR, sequencing, and multiplex PCR were used for the detection of blaOXA-23-like, blaOXA-51-like, blaOXA-24-like, and blaOXA-58-like genes, which were then sequenced. The ISAba1 gene was detected, and PCR was performed to detect the presence of ISAba1/blaOXA-51-like and ISAba1/blaOXA-23-like genes. Results:The results showed that 93% of the strains were multi-drug resistant, while 82% of them were extensively drug resistant. Additionally, all the strains carried blaOXA-23-like and blaOXA-51-like genes, while 74% and 0% of the strains harbored blaOXA-24-like and blaOXA-58 genes, respectively. ISAba1 was detected in all the strains except for one. The co-existence of ISAba1/blaOXA-51-like genes and ISAba1/blaOXA-23-like genes was detected in 65% and 80% of strains, respectively. Conclusions:The results of this study indicate that the emergence of OXA-type carbapenemases in A. baumannii causing nosocomial infections in burns patients could be of importance for hospital infection control systems in Iran.

show abstract

Section: Discussionsupporting

confidence: 92%

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Mohammadi

Goudarzi

Hashemi

et al. 2016

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

show abstract

“…In addition, the ISAba1 insertion sequence in the upstream of the blaOXA-51 was seen in 32% isolates. The results of Ba- hador et al are similar to our results; however, the results of Salimizand et al are higher (100%) than this studys' results (27,28).…”

Section: Discussionsupporting

confidence: 34%

Polyclonal Distribution of blaOXA-23 Gene Among Acinetobacter baumannii Isolated from Intensive Care Unit Patients in Tehran; Pulsed-Field Gel Electrophoresis Analysis

Dehbalaei

Peerayeh

Behmanesh

et al. 2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Despite a relatively low virulence of Acinetobacter baumannii isolates, emerging multidrug-resistant (MDR) strains to pose a formidable threat to patients, particularly in ill patients in intensive care unit (ICU). Objectives: The aim of the present study was to determine the genetic relatedness and antimicrobial susceptibility patterns in the endemic clones of A. baumannii isolated from patients in the ICU. Methods: Fifty-five non-repetitive A. baumannii isolates were examined for antimicrobial susceptibility, oxacillinase genes, class 1 integrons and genetic relationships by PFGE. Results: Antibiotic susceptibility testing showed that 21.8%) 12 isolates) were resistant to all tested antibiotics. Resistant to carbapenems were up to 85%. OXA-23, OXA-24 and OXA-58 genes were detected in 81.81%, 16.36% and 1.81% isolates respectively. The ISAba1 element upstream of blaOXA-51 was detected in 18 (32%) isolates, and 22 (40%) isolates had an ISAba1 insertion sequence upstream of the blaOXA-23. Integron class 1 was detected in 25 (55.5%) OXA-23 carrying isolates, 2 (22.2%) in OXA-24 positive isolates and in one OXA-58 carrying isolate. PFGE analysis resolved 50 distinct pulsotypes, Most of the isolates were scattered throughout across the dendrogram and a few grouped as clusters. Conclusions: No significant association has been found between the pulsotype of each isolate and MDR patterns and the presence of carbapenemase genes, however, highly resistant blaOXA-23 gene carrying endemic clones of A. baumannii disseminated in the ICU of two hospitals. Therefore, active surveillance and health policies are urgently needed for the detection and control the dissemination of such organisms.

show abstract

“…World health organization (WHO) declared that Multidrug resistance (MDR) strains of Acinetobacter baumannii are a significant threat worldwide. In total, many studies have shown that the prevalence of MDR strains of A. baumannii has reached an alarming statue all over the world (9), including Iran (10,11). Developing countries such as Iran have serious problems in treating the infections resulted from MDR strains.…”

Section: Introductionmentioning

confidence: 99%

Increasing Trend of Imipenem-Resistance Among Acinetobacter baumannii Isolated From Hospital Acquired Pneumonia in Northeast of Iran

Khaledi¹,

Elahifar²,

Vazini³

et al. 2017

Avicenna J Clin Microbiol Infect

Self Cite

View full text Add to dashboard Cite

show abstract

The Prevalence of ISAba1 and ISAba4 in Acinetobacter baumannii Species of Different International Clone Lineages Among Patients With Burning in Tehran, Iran

Cited by 27 publications

References 26 publications

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Polyclonal Distribution of blaOXA-23 Gene Among Acinetobacter baumannii Isolated from Intensive Care Unit Patients in Tehran; Pulsed-Field Gel Electrophoresis Analysis

Increasing Trend of Imipenem-Resistance Among Acinetobacter baumannii Isolated From Hospital Acquired Pneumonia in Northeast of Iran

Contact Info

Product

Resources

About