The Prevalence of unexpected antibodies in Saudi's plasma prior blood transfusion and their association with clinical conditions: A cross-sectional study

Waggiallah, Hisham Ali; Alenzi, Faris Q.; Shaya, Abdulkarim S. Bin; Hattan, Ahmed Hattan; Elmosaad, Yousif Mohammed; Al-Enazi, Maher M.

doi:10.1016/j.sjbs.2021.04.083

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…This indicates that there may be discrepancies in results between different regions and different systems with various analyzers and methods. RH blood group system accounts for the largest proportion of UAb 15 . The significance of RhD blood group in clinical blood transfusion is second only to ABO blood group.…”

Section: Discussionmentioning

confidence: 99%

Establishment and application of suspension static method in blood group screening of automated blood group analyzer

Huang

et al. 2023

Sci Rep

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The accuracy of blood group identification is the basis of blood transfusion safety. In order to increase the detection rate of weak agglutination, unexpected antibodies (UAb) and blood subtypes for pre-transfusion testing, the blood group screening process of automated blood group analyzer (ABGA) is ameliorated by introducing one static step and establishing a suspension static method (SSM). One static step was introduced in the blood group screening process of ABGA and three static time conditions were designed: 300 s, 400 s and 500 s, from which the optimal static time was selected and SSM was established; By comparing the detection of weak agglutination and UAb before and after the application of SSM, the feasibility and effect of suspension static method were verified and evaluated. The last two steps of the automatic blood group screening process were replaced with static, light centrifugation and imaging. The optimal static time parameter was selected as 400 s and SSM was established; After the application of SSM, it was verified that: (1) The detection level of weak antibodies (anti-A and anti-B) and weak antigens (weak D phenotype) could be improved by SSM, including antibodies in plasma of known type O samples with 0, 2, 4, 8, 16 and 32 times serial dilutions(simulating weak anti-A and weak anti-B), weak antibodies (anti-B) in plasma of one normal A-type sample and weak antigens on red blood cells (RBC) of 5 weak D phenotype samples (weak D antigen); (2) Three blood donor samples (type A, O and B) with known UAb were detected by SSM. The results showed that SSM could detect both weak antibodies (anti-A and anti-B) and UAb; (3) SSM was applied to detect the samples of 3 A2B and 3 subtype B blood donors and the blood subtypes could be clearly detected; (4) The number of screening samples was 95,314 and 106,814 before SSM (2018) and after (2020) the application of SSM and the positive rate of UAb (63/95,314 and 187/106,814) increased after SSM, discrepancy of which was statistically significant (χ2 = 48.42, P < 0.01). The above results demonstrate that SSM of ABGA is conducive to the detection of weak agglutination, UAb and blood subtypes in blood samples, which can improve the sensitivity of blood group detection and ensure the safety of clinical blood transfusion to a certain extent.

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Section: Discussionmentioning

confidence: 99%

Establishment and application of suspension static method in blood group screening of automated blood group analyzer

Huang

et al. 2023

Sci Rep

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Characteristics of unexpected antibodies in patients with blood disorders: Evidence in Vietnam

Binh,

Thi Thanh Nga,

Thanh

et al. 2024

Transfusion and Apheresis Science

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Investigation of Domestic and Foreign Unexpected Antibodies for Emergency Blood Transfusion

Hwang

Lee

Park

2022

Korean J Clin Lab Sci

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Certain pre-transfusion tests are not commonly performed during emergency blood transfusion. In this study, we reviewed and analyzed the data of post-blood transfusion antibody screening tests to establish the effects of unexpected antibodies causing hemolytic transfusion reactions. We reviewed information published domestically and internationally, and selected the data of 68,602 antibody screening tests and 528 antibody identification tests conducted at P hospital. We found that unexpected antibody positive (1198,1.74%), Rh type (161, 30.49%), Lewis type (67, 12.69%), others (Di (a), 28, 5.30%). The anti-E type positive was 93 (17.61%), and that of the cases with anti-C (13, 2.46%). Only data of domestic cases were included for analysis that were published before 2007, which established the presence of antibodies of the following types and numbers of cases: anti-E (196, 22.45%), anti-Le a (82, 9.39%), and anti-E+C (60, 6.87%). In 2018, anti-E (107, 17.12%), anti-E+C (56, 8.96%), and anti-Di a (28, 4.48%) were detected. In other domestic cases, S hospital was detect to anti-E, anti-Le a, anti-E+C. The Anti-E, anti-D, anti-E+C, and anti-C+E were detected in D hospital. In Saudi Arabia, Anti-D, anti-E, and anti-Jka was detected. The Anti-M, Anti-N, Anti-Le (a), and Anti-D were detected in India. Requests for emergency blood transfusion increased 1.8 times after the opening of the trauma center. This study has the disadvantage of being a cross-sectional study. additional studies are needed to provide basic information on alternative treatments that can increase the safety and reduce the side effects of hemolytic transfusion in emergency transfusion situations.

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The Prevalence of unexpected antibodies in Saudi's plasma prior blood transfusion and their association with clinical conditions: A cross-sectional study

Cited by 4 publications

References 16 publications

Establishment and application of suspension static method in blood group screening of automated blood group analyzer

Establishment and application of suspension static method in blood group screening of automated blood group analyzer

Characteristics of unexpected antibodies in patients with blood disorders: Evidence in Vietnam

Investigation of Domestic and Foreign Unexpected Antibodies for Emergency Blood Transfusion

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