The preventive effect of Apocynum�venetum polyphenols on D‑galactose‑induced oxidative stress in mice

Abstract

Apocynum venetum is a traditional medicine that is rich in polyphenols. Apocynum venetum polyphenol extract (AVP) contains the active substances neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, astragaloside and rosmarinic acid. In the present study, the preventive effect of AVP against D-galactose-induced oxidative stress was studied in a mouse model. The sera, skin, livers and spleens of mice were examined using hematoxylin and eosin staining, reverse transcription-quantitative PCR and western blot analysis. The biochemical results showed that AVP improved the thymus, brain, heart, liver, spleen and kidney indices in a mouse model of oxidative stress. AVP was also able to reverse the reduction in levels of superoxide dismutase (SOD), glutathione peroxidase and glutathione, and increased the levels of nitric oxide and malondialdehyde identified in the serum, liver, spleen and brain of mice exposed to oxidative stress. Pathological observations confirmed that AVP could inhibit oxidative damage to the skin, liver and spleen of mice caused by D-galactose. Further molecular biological experiments also demonstrated that AVP increased the expression of neuronal nitric oxide synthase, endothelial nitric oxide synthase, Cu/Zn-SOD, Mn-SOD, catalase, heme oxygenase-1, nuclear factor-erythroid 2-related factor 2, γ-glutamylcysteine synthetase and NAD(P)H quinone dehydrogenase 1 and reduced the expression of inducible nitric oxide synthase in the liver and spleen of treated mice compared to controls. Notably, the preventive effect of AVP against D-galactose-induced oxidative damage in mice was better than that of the confirmed antioxidant vitamin C. In conclusion, AVP exhibited an antioxidant effect and the AVP-rich Apocynum venetum may be considered a plant resource with potential antioxidative benefits.

Determination of Polyphenols in Ilex kudingcha and Insect Tea (Leaves Altered by Animals) by Ultra-high-performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (UHPLC-QqQ-MS) and Comparison of Their Anti-Aging Effects

“…Compared with the model group, IKDCP and ITP upregulated the expression of Cu/Zn-SOD, Mn-SOD, CAT, GSH1 , and increased GSH-PX mRNA and protein, indicating that the free radicals produced by oxidative metabolism in the liver, skin, and spleen tissue of aging mice induced by D-galactose can be eliminated. An anti-oxidant effect was observed after ITP treatment, which upregulated the expression of anti-oxidant-related genes and proteins so that they were similar to that of normal organisms, which is consistent with the results of Guo et al (2020) and Yi et al (2019) .…”

Galactose-Induced Skin Aging: The Role of Oxidative Stress

“…Aging models based on the administration of D-gal have been intensively utilized for validation of the antiaging efficacy of natural antioxidant compounds over the last decades [148]. In fact, the antioxidant properties of polyphenols have been tested in a number of studies using a model of skin aging based on the use of D-gal [149][150][151][152]. For instance, it was established that the combined treatment using resveratrol and calorie restriction restores hair condition, skin elasticity, and skin thickness in rats treated by D-gal [85].…”

Incorporation of Rapa Whelk (Rapana venosa) meal in diets for rainbow trout (Oncorhynchus mykiss) fry

“…The organ weight and indices are parameters that indicate the health status of an organism (Guo et al., 2020). It has previously been reported by Pan et al.…”

“…(2018) that the liver is an immune‐related organ, and according to Khan et al. (2017), any change in the hepatic index can be correlated to immunity parameters (Guo et al., 2020). Considering our findings, the HSI values in fish were similar in all treatment groups, while VSI was higher only in the CD treatment compared with the other groups.…”