The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/K_ATPSignaling Pathway

Abstract

Plumeria rubra (Apocynaceae) is frequently used in folk medicine for the treatment of gastrointestinal disorders, hepatitis, and tracheitis, among other infirmities. The aim of this study was to investigate the gastroprotective potential of a protein fraction isolated from the latex of Plumeria rubra (PrLP) against ethanol-induced gastric lesions and describe the underlying mechanisms. In a dose-dependent manner, the pretreatment with PrLP prevented ethanol-induced gastric lesions in mice after single intravenous administration. The gastroprotective mechanism of PrLP was associated with the involvement of prostaglandins and balance of oxidant/antioxidant factors. Secondarily, the NO/cGMP/KATP pathway and activation of capsaicin-sensitive primary afferents were also demonstrated as part of the mechanism. This study shows that proteins extracted from the latex of P. rubra prevent gastric lesions induced in experimental animals. Also, the results support the use of the plant in folk medicine.

Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study

“…There were some exfoliated cells appearing in the lumen, besides, intraepithelial lymphocytes and extra-vasated RBCs. These results were in accordance with some investigators [1,[27][28][29] , who reported that the ethanol had direct effect on gastric mucosa and had ability to induce necrosis of the surface gastric epithelial cells and also erosion. The presence of inflammatory cells infiltration and intra epithelial lymphocytes in gastric mucosa indicates ongoing inflammation.…”

Purification, Partial Characterization, and Evaluation of the Antiulcer Activity of Calotropis procera Leaf Lectin

“…In addition, they have confirmed the safety of the total alcoholic extract at up to 4000 mg/kg [ 39 ]. Ethanol gastric-inducedlesions are multifactorial and are linked to the reduction in the inherent stomach mucosa defensemechanism and that’s principally associated with the change in the microcirculation or maybe the oxidation stress [ 41 ]. To test for the possible cytoprotective activity of the purified ProLec, we initially performed a pilot study using a wide range of ProLec concentrations ranging from 0.25 mg to 1 mg/kg, excitingly all of these protein ranges showed, a dose-dependent, remarkable protective effect against ethanol-induced gastric lesion on experimental animals.…”