The primary cilium of telocytes in the vasculature: electron microscope imaging

Cantarero, Irene; Luesma, María José; Junquera, Concepción

doi:10.1111/j.1582-4934.2011.01312.x

Cited by 68 publications

(60 citation statements)

References 36 publications

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“…Generally, vascular TCs seem to have a typical morphology, with some exceptions (45,46). Cell bodies of TCs located in aortic arch were smaller (range from 6.06 μm to 13.02 μm in length and from 1.05 μm to 4.25 μm in width), with high nuclear/cytoplasm ratio (47).…”

Section: Cell Morphology and Distribution In Vascular Systemmentioning

confidence: 99%

“…So far, immunohistochemical markers used for identifi cation of vascular TCs include CD34, vimentin and PDGFR-β (17,45). Expression of c-kit (CD117), along with Nanog and Sca-1 (which are histochemical markers characteristic for stem cells) was also confi rmed in vascular telocytes (17).…”

Section: Markers Of Vascular Tcsmentioning

confidence: 99%

“…They are connected with each other through gap junctions or desmosomes (45,47,49). TCs accompanying capillaries frequently establish heterocellular contacts with pericytes and endothelial cells.…”

Section: Intercellular Junctionsmentioning

confidence: 99%

“…Moreover, Cantarero et al reported a unique feature of vascular TCs, namely a primary cilium. This immotile cilium contains 9+0 axoneme, as opposed to 9+2 axoneme of majority of motile cilia (45).…”

Section: Cell Morphology and Distribution In Vascular Systemmentioning

confidence: 99%

“…In tunica adventitia, cell bodies of TCs are arranged parallelly to the longitudinal axis of the vessel, extending their Tps to form a convoluted network (45,47). In innermost part of intima, TCs exist either in groups or as a single cell, occasionally extending their Tps into the lumen of the vessel (46).…”

Section: Cell Morphology and Distribution In Vascular Systemmentioning

confidence: 99%

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A comprehensive guide to telocytes and their great potential in cardiovascular system

Kucybała¹,

Janas²,

Ciuk³

et al. 2017

BLL

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Telocytes, a recently discovered type of interstitial cells, have a very distinctive morphology -the small cell body with long extensions, named telopodes. In our review, apart from introducing general aspects of telocytes, we focus on properties, functions and future potential of those cells in cardiovascular system. However, physiological functions of telocytes in cardiovascular system are still regarded as quite enigmatic. Previous studies claim that they play a role in organogenesis and regeneration, bioelectrical signalling, mechanoelectrical coupling, anti-oxidative protection, angiogenesis and regulation of blood fl ow. As well, they are presumably connected with the presence of blood-myocardium barrier and proper organisation of extracellular matrix. Moreover, there exists a signifi cant link between the quantity of telocytes in tissue and numerous cardiovascular diseases such as: myocardial infarction, cardiomyopathies, systemic sclerosis, heart failure, atrial fi brillation, isolated atrial amyloidosis, myxomatous valve degeneration and hyperplastic consequences of vascular injury. Thanks to their unique properties, telocytes might be a breakthrough in treatment of cardiovascular diseases, as they may be effective in reversing effects of myocardial infarction. Telocytes also may play a major role in tissue engineering -they might be the key factor in creating stable and effi cient vascular network in larger synthetic tissues or organs (Tab. 1, Fig. 3, Ref. 53). Text in PDF www.elis.sk.

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Section: Cell Morphology and Distribution In Vascular Systemmentioning

confidence: 99%

Section: Markers Of Vascular Tcsmentioning

confidence: 99%

Section: Intercellular Junctionsmentioning

confidence: 99%

Section: Cell Morphology and Distribution In Vascular Systemmentioning

confidence: 99%

Section: Cell Morphology and Distribution In Vascular Systemmentioning

confidence: 99%

See 3 more Smart Citations

A comprehensive guide to telocytes and their great potential in cardiovascular system

Kucybała¹,

Janas²,

Ciuk³

et al. 2017

BLL

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Immunohistochemical biomarkers and distribution of telocytes in ApoE^−/− mice

Tian

Cheng

et al. 2019

Cell Biology International

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Telocytes had been identified as a peculiar stromal cell type implicated in tissue homeostasis and the development and pathophysiology of diseases. Telocyte existed in most organs and tissues in humans and animals. However, few studies have examined telocytes in ApoE gene deficient mice. In our studies, we verified the existence, the morphology and immunohistochemical characteristics of telocytes in critical organs of the ApoE−/− mice. Male adult ApoE−/− mice were selected as an experimental model. Immunohistochemical bio‐markers, such as CD34, CD117, CD28, Vimentin and PDGFR‐α were utilized to determine the distribution and morphology of telocytes in the heart, liver and kidney. Telocyte expressed positively for CD34 and CD117, and partial telocyte and telopode expressed positively for PDGFR‐α in heart and liver, but negatively in kidney. Double immunofluorescence assays for CD28/Vimentin, CD34/CD117 and CD34/PDGFR‐α were used to demonstrate the biochemistry speciality of telocytes, respectively. The evidence of telocytes in the ApoE‐/‐ mice is the first step of our sturdy, which aims to demonstrate changes in telocytes in atherosclerosis in this animal model.

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CD34⁺ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence

Dama,

Xue,

Zhang

et al. 2024

Cell Biology International

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Telocytes (TCs), a novel type of mesenchymal or interstitial cell with specific, very long and thin cellular prolongations, have been found in various mammalian organs and have potential biological functions. However, their existence during lung development is poorly understood. This study aimed to investigate the existence, morphological features, and role of CD34+ SCs/TCs in mouse lungs from foetal to postnatal life using primary cell culture, double immunofluorescence, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The immunofluorescence double staining profiles revealed positive expression of CD34 and PDGFR‐α, Sca‐1 or VEGFR‐3, and the expression of these markers differed among the age groups during lung development. Intriguingly, in the E18.5 stage of development, along with the CD34+ SCs/TCs, haematopoietic stem cells and angiogenic factors were also significantly increased in number compared with those in the E14.5, E16.5, P0 and P7. Subsequently, TEM confirmed that CD34+ SCs/TCs consisted of a small cell body with long telopodes (Tps) that projected from the cytoplasm. Tps consisted of alternating thin and thick segments known as podomers and podoms. TCs contain abundant endoplasmic reticulum, mitochondria and secretory vesicles and establish close connections with neighbouring cells. Furthermore, SEM revealed characteristic features, including triangular, oval, spherical, or fusiform cell bodies with extensive cellular prolongations, depending on the number of Tps. Our findings provide evidence for the existence of CD34+ SCs/TCs, which contribute to vasculogenesis, the formation of the air‒blood barrier, tissue organization during lung development and homoeostasis.

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The primary cilium of telocytes in the vasculature: electron microscope imaging

Cited by 68 publications

References 36 publications

A comprehensive guide to telocytes and their great potential in cardiovascular system

A comprehensive guide to telocytes and their great potential in cardiovascular system

Immunohistochemical biomarkers and distribution of telocytes in ApoE^−/− mice

CD34⁺ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence

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The primary cilium of telocytes in the vasculature: electron microscope imaging

Cited by 68 publications

References 36 publications

A comprehensive guide to telocytes and their great potential in cardiovascular system

A comprehensive guide to telocytes and their great potential in cardiovascular system

Immunohistochemical biomarkers and distribution of telocytes in ApoE−/− mice

CD34+ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence

Contact Info

Product

Resources

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Immunohistochemical biomarkers and distribution of telocytes in ApoE^−/− mice

CD34⁺ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence