The Primary Structure of the Cl−-Translocating ATPase, b Subunit ofAcetabularia acetabulum,Which Belongs to the F-Type ATPase Family

Moritani, Chie; Ohhashi, Toshitaka; Kadowaki, Hiromi; Tagaya, Mitsuo; Fukui, Tsuguya; Lottspeich, Friedrich; Oesterhelt, Dieter; Ikeda, Mikiko

doi:10.1006/abbi.1996.9845

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…Recently, similar molecular masses have been obtained for Cl x pump catalytic units in alga (Ikeda & Oesterhelt, 1990;Moritani et al, 1994) and rat brain Zeng et al, 1998) confirming the possible E 1 -E 2 nature of the ATPase although the authors of these studies postulate these structural subunits to be part of a V-ATPase assembly, despite the ATPases being inhibitable by the P-ATPase inhibitor vanadate Moritani et al, 1994). The molecular masses of both the rat brain (Zeng et al, 1998;Inagaki et al, 2001) and alga (Moritani et al, 1994;Moritani et al, 1997) Cl x -ATPase complexes exceeded 500 kDa.…”

Section: Reconstitution Of the CL X Pumpsupporting

confidence: 52%

“…(2) Demonstrations of reconstitution and phosphorylation of Cl x -ATPase have been discussed in the present review which provide the first direct evidence for the existence of a new P-type ATPase: the Cl x pump. However, it appears that V-and F-type Cl x -ATPases also exist as Cl x pump mechanisms (Moritani et al, 1997;Zeng et al, 1998) along with the photondriven halorhopsins (Kitajima et al, 1996). (3) The variety and number of Cl x pumps attests to the adaptability of a particular organism existing in a particular environment and not to the non-existence of this primary active transporter in nature.…”

Section: Discussionmentioning

confidence: 99%

“…The genes encoding the b subunit (50 kDa) of the Cl x translocating ATPase of Acetabularia acetabulum were cloned from total RNA and from poly(A) + RNA and sequenced. The deduced amino acid sequence of the open reading frame consisted of 478 amino acids and showed high similarity to the b-subunit of chloroplast F 1 -ATPases (Moritani et al, 1997). Gene fragments encoding the putative b-subunit of chloroplast F 1 -(273 bp) and mitochondrial F 1 -ATPases (332 bp) were also cloned from Acetabularia acetabulum and sequenced, respectively.…”

Section: Molecular Biology Of the CL X Pumpmentioning

confidence: 99%

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Chloride ATPase pumps in nature: do they exist?

Gerencser

Zhang

2003

Biological Reviews

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Five widely documented mechanisms for chloride transport across biological membranes are known: anion-coupled antiport, Na+ and H(+)-coupled symport, Cl- channels and an electrochemical coupling process. These transport processes for chloride are either secondarily active or are driven by the electrochemical gradient for chloride. Until recently, the evidence in favour of a primary active transport mechanism for chloride has been inconclusive despite numerous reports of cellular Cl(-)-stimulated ATPases coexisting, in the same tissue, with uphill ATP-dependent chloride transport. Cl(-)-stimulated ATPase activity is a ubiquitous property of practically all cells with the major location being of mitochondrial origin. It also appears that plasma membranes are sites of Cl(-)-stimulated ATPase pump activity. Recent studies of Cl(-) -stimulated ATPase activity and ATP-dependent chloride transport in the same plasma membrane system, including liposomes, strongly suggest a mediation by the ATPase in the net movement of chloride up its electrochemical gradient across the plasma membrane structure. Contemporary evidence points to the existence of Cl(-)-ATPase pumps; however, these primary active transporters exist as either P-, F- or V-type ATPase pumps depending upon the tissue under study.

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Section: Reconstitution Of the CL X Pumpsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Molecular Biology Of the CL X Pumpmentioning

confidence: 99%

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Chloride ATPase pumps in nature: do they exist?

Gerencser

Zhang

2003

Biological Reviews

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“…[19] However, evidences for the molecular nature and mechanism of ATP-driven chloride transport are still mostly indirect. [20] Another active chloride transporter that is commonly found in Haloarchaea is halorhodopsin. [20] Another active chloride transporter that is commonly found in Haloarchaea is halorhodopsin.…”

Section: Overview Of Halide-specific Membrane Transport Systemsmentioning

confidence: 99%

Halide Transport through Biological Membranes

Essen¹

2014

Dekker Encyclopedia of Nanoscience and Nanotechnology, Third Edition

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“…Given this long-distance relationship, it is hard to envision that the cytoskeleton (78) and molecular motors would not be intimately involved in moving and targeting information for aspects of pole establishment, differentiation, or maintenance of body regions. Acetabularia have sizable internal currents (see references in 83), but whether such currents play any role in morphogenesis in this plant is unknown. Perhaps the physical distance between the poles of A. acetabulum provides a clue about why the data support translational rather than transcriptional regulation of reproductive onset.…”

Section: Potential For Future Insights Into Development and Morphogenmentioning

confidence: 99%

ELABORATION OF BODY PLAN AND PHASE CHANGE DURING DEVELOPMENT OF ACETABULARIA: How Is the Complex Architecture of a Giant Unicell Built?

Mandoli

1998

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

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While uninucleate and unicellular, Acetabularia acetabulum establishes and maintains functionally and morphologically distinct body regions and executes phase changes like those in vascular plants. Centimeters tall at maturity, this species has allowed unusual experimental approaches. Amputations revealed fates of nucleate and enucleate portions from both wild type and mutants. Historically, graft chimeras between nucleate and enucleate portions suggested that morphological instructions were supplied by the nucleus but resided in the cytoplasm and could be expressed interspecifically. Recently, graft chimeras enabled rescue of mutants arrested in vegetative phase. Since the 1930s, when Acetabularia provided the first evidence for the existence of mRNAs, a dogma has arisen that it uses long-lived mRNAs to effect morphogenesis. While the evidence favors translational control, the postulated mRNAs have not been identified, and the mechanism of morphogenesis remains unknown. Amenable to biochemistry, physiology, and both classical and molecular genetics, Acetabularia may contribute yet new insights into plant development and morphogenesis.

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The Primary Structure of the Cl−-Translocating ATPase, b Subunit ofAcetabularia acetabulum,Which Belongs to the F-Type ATPase Family

Cited by 10 publications

References 37 publications

Chloride ATPase pumps in nature: do they exist?

Chloride ATPase pumps in nature: do they exist?

Halide Transport through Biological Membranes

ELABORATION OF BODY PLAN AND PHASE CHANGE DURING DEVELOPMENT OF ACETABULARIA: How Is the Complex Architecture of a Giant Unicell Built?

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