The Principles of Freeze-Drying

Adams, G.D.; Cook, Isobel; Ward, Kevin

doi:10.1007/978-1-4939-2193-5_4

Cited by 36 publications

(33 citation statements)

References 31 publications

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“…Lyophilization (freeze drying) is a conservation process involving both freezing and dehydration. This technique is commonly used for preservation of several biological products, bacterial and viral cultures, as well as foods and drugs, to maintain stability and viability for prolonged intervals (Adams, ). Lyophilization could be an interesting long‐term method for storing EYP because the water fraction present in the liquid form may function as a substrate or medium for many lipid oxidation reactions (Obara, Obiedzinski, & Kolczak, ).…”

Section: Discussionmentioning

confidence: 99%

A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa

Belala

Briand-Amirat

Martinot

et al. 2019

Reprod Domestic Animals

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The current study aimed to explore the potential usefulness of liquid or lyophilized egg yolk plasma (EYP) as a substitute for low‐density lipoproteins (LDL) for cryopreservation of canine spermatozoa. In the first experiment, a total of 20 ejaculates harvested from six Beagles were frozen in extenders containing 6% LDL (control) or liquid or lyophilized EYP at one of three concentrations (20%, 40% or 60%). Motility parameters were assessed 10 min after thawing using computer‐assisted sperm analysis. For both liquid and lyophilized EYP, the 40% concentration yielded motility similar (p > 0.05) to that observed with the control extender. In the second experiment, 12 ejaculates collected from the same six dogs were frozen in 6% LDL (Control), 40% liquid EYP or 40% lyophilized EYP extenders. Spermatozoal membrane integrity (hypo‐osmotic swelling test [HOSt] and SYBR14/propidium iodide [PI] staining), acrosome integrity (FITC‐Pisum sativum agglutinin staining) and DNA integrity (acridine orange staining) characteristics were evaluated 10 min after thawing. Both liquid and lyophilized 40% EYP‐based extenders successfully preserved all assessed integrity parameters as efficiently as the control. Results of this study suggest that lyophilized EYP is a viable alternative to LDL in freezing extenders for dog semen.

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Section: Discussionmentioning

confidence: 99%

A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa

Belala

Briand-Amirat

Martinot

et al. 2019

Reprod Domestic Animals

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“…It protects the structure and avoids denaturation of protein in the cells during drying. In addition, sucrose able to forming a glass state in dry tissue, avoids the cells to burst and deterioration [22]. The viability of the cells was improved by addition of tween 80 in the solution.…”

Section: A Cell Viability Studymentioning

confidence: 99%

Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant

Azoddein¹,

Nuratri²,

Azli³

et al. 2017

AIP Conference Proceedings

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Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and high stability of the product. This study was aimed to freeze dry P. putida cells with addition of lyoprotectant. Lyoprotectant was added into the cells suspension prior to freezing. Dried P. putida obtained was then mixed with synthetic mercury. Viability of recovery P. putida after freeze dry was significantly influenced by the type of lyoprotectant. Among the lyoprotectants, tween 80/ sucrose was found to be the best lyoprotectant. Sucrose able to recover more than 78% (6.2E+09 CFU/ml) of the original cells (7.90E+09CFU/ml) after freeze dry and able to retain 5.40E+05 viable cells after 4 weeks storage in 4oC without vacuum. Polyethylene glycol (PEG) pre-treated freeze dry cells and broth pre-treated freeze dry cells after freeze-dry recovered more than 64% (5.0 E+09CFU/ml) and >0.1% (5.60E+07CFU/ml). Freeze-dried P. putida cells in PEG and broth cannot survive after 4 weeks storage. Freeze dry also does not really change the pattern of growth P. putida but extension of lag time was found 1 hour after 3 weeks of storage. Additional time was required for freeze-dried P. putida cells to recover before introduce freeze-dried cells to more complicated condition such as mercury solution. The maximum mercury reduction of PEG pre-treated freeze-dried cells after freeze dry and after storage 3 weeks was 56.78% and 17.91%. The maximum of mercury reduction of tween 80/sucrose pre-treated freeze-dried cells after freeze dry and after storage 3 weeks were 26.35% and 25.03%. Freeze dried P. putida was found to have lower mercury reduction compare to the fresh P. putida that has been growth in agar. Result from this study may be beneficial and useful as initial reference before commercialize freeze-dried P. putida.

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“…Liquid working seed virus turned to open, porous and dry cake after lyophilization due to primary drying by sublimation of the ice crystal from frozen material and secondary drying or desorption by evaporation of the free water adsorbed into the dried product (Asim et al 2008). Haemagglutination titer and EID 50 /ml of working seed virus after lyophilization decreased due to the death and autolyisis of some virus during lyophilization for cold shock (Adams, 2007). LAS and PVP cryoprotectant in preparation of vaccine the losses of EID 50 / ml after lyophilization was 0.51 and 0.53 respectively.…”

mentioning

confidence: 99%

“…Lyophilization which also termed as freeze-drying was a controllable method of dehydrating labile products by vacuum desiccation. In preparation of live attenuated freeze dried vaccine lyophilization is an important step where we stabilize viruses by cooling of the liquid sample and two overlapping drying procedure (Adams, 2007). Cryoprotectant was needed for virus stabilization which preserves and protects the virus against injury due to reduction of temperature at the time of lyophilization (Pegg, 2007).…”

mentioning

confidence: 99%

“…Ltd., India) and solution of 1% polyvinyl pyrrolidone (Sigma Aldrich Co., USA) were used as two different types of cryoprotectant. Then processing within freeze dryer machine was started (Adams, 2007). After completing the lyophilization or freeze drying vacuum test of the glass vials was done with vacuum torch in dark room and a bluish light was seen within the vaccine vials in positive cases.…”

mentioning

confidence: 99%

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Comparative efficacy of cryoprotectant in the lyophilization of pigeon paramyxovirus-1 vaccine

Das

Isore

Guha

et al. 2017

IJAR

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Preparation of live attenuated freeze dried Pigeon Paramyxo Virus-1 (PPMV-1) vaccine selection of proper cryoprotectant which could preserve and protect the virus against injury due to reduction of temperature at the time of lyophilization, was very necessary. PPMV-1 locally isolated lentogenic strain was selected as seed virus which was properly attenuated by serially passaging in 9 to 10 days old embryonated specific pathogen free (SPF) fowl eggs and produced master seed virus and working seed virus. Five per cent lactalbumin hydrolysate with 10% sucrose (LAS) and 1% polyvinyl pyrrolidone (PVP) were used as two different cryoprotectant for lyophilization of working seed virus. After lyophilization value of haemagglutination (HA) titer and embryo infective dose fifty per ml (EID50/ml) were changed and concluded that both LAS and PVP could be used as suitable cryoprotectant where LAS was better than PVP which was first time done in the whole world.

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The Principles of Freeze-Drying

Cited by 36 publications

References 31 publications

A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa

A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa

Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant

Comparative efficacy of cryoprotectant in the lyophilization of pigeon paramyxovirus-1 vaccine

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