The proangiogenic potential of a novel calcium releasing biomaterial: Impact on cell recruitment

Oliveira, Hugo; Catros, Sylvain; Boiziau, Claudine; Siadous, Robin; Martí-Muñoz, Joan; Bareille, Reine; Rey, Sylvie; Castaño, Óscar; Planell, Josep A.; Amédée, Joëlle; Engel, Elisabeth

doi:10.1016/j.actbio.2015.10.003

Cited by 42 publications

(62 citation statements)

References 45 publications

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“…Also, PLA is often used in association with calcium phosphate particles to improve bone regeneration . Scaffolds loaded by calcium phosphate nanoparticles could be used to promote osteoblast activity and bone tissue formation …”

Section: Discussionmentioning

confidence: 99%

Characterization of printed PLA scaffolds for bone tissue engineering

Grémare

Gudurić

Bareille

et al. 2017

J Biomedical Materials Res

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Autografts remain the gold standard for orthopedic transplantations. However, to overcome its limitations, bone tissue engineering proposes new strategies. This includes the development of new biomaterials such as synthetic polymers, to serve as scaffold for tissue production. The objective of this present study was to produce poly(lactic) acid (PLA) scaffolds of different pore size using fused deposition modeling (FDM) technique and to evaluate their physicochemical and biological properties. Structural, chemical, mechanical, and biological characterizations were performed. We successfully fabricated scaffolds of three different pore sizes. However, the pore dimensions were slightly smaller than expected. We found that the 3D printing process induced decreases in both, PLA molecular weight and degradation temperatures, but did not change the semicrystalline structure of the polymer. We did not observe any effect of pore size on the mechanical properties of produced scaffolds. After the sterilization by γ irradiation, scaffolds did not exhibit any cytotoxicity towards human bone marrow stromal cells (HBMSC). Finally, after three and seven days of culture, HBMSC showed high viability and homogenous distribution irrespective of pore size. Thus, these results suggest that FDM technology is a fast and reproducible technique that can be used to fabricate tridimensional custom-made scaffolds for tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 887-894, 2018.

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Section: Discussionmentioning

confidence: 99%

Characterization of printed PLA scaffolds for bone tissue engineering

Grémare

Gudurić

Bareille

et al. 2017

J Biomedical Materials Res

Self Cite

278

180

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“…Calcium release from Si-based ormoglasses has been shown to stimulate a cell-membrane calcium sensing receptor acting as effective angiogenic promoter [26]. Another recent study showed that by altering the release of calcium from electrospun PLA fiber-based membranes containing different ratios of calcium phosphate ormoglass particles in vivo, it is possible to induce a chemotactic effect on macrophages to regulate the angiogenic progression of events [27]. Although a significant difference in the secretion of VEGF was not observed in the present work, the highest release of VEGF was found for the 1 wt/vol.% concentration of SmS granules.…”

Section: Discussionmentioning

confidence: 99%

Influence of dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells

Balasubramanian¹,

Detsch²,

Esteban-Tejeda³

et al. 2017

Biomedical Glasses

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This study evaluated the influence of ionic dissolution products of a novel Ca-enriched silicate bioactive glass compared to commercial available hydroxyapaptite samples (Endobon ) on cell activity and vascular endothelial growth factor (VEGF) release in vitro. Bone marrow stromal cells (ST-2) were cultivated with the supernatant of granules of different sizes and at different concentrations (0-1 wt/vol % of granules) for 48 h. In addition to in vitro studies, Ca-ion release from all granules was analysed as the dissolution product of interest for the investigated glass. Cell viability studies as well as cell morphology observation revealed no cytotoxic effect of the released products from all tested materials. It was found that supernatants from granules in concentrations of 1 wt/vol % enhanced the VEGF release from ST2 cells, which is important as a marker of the vascularisation ability of the glass during the bone healing process.

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“…Immunohistochemistry for CD31 was performed as previously described 34 . Briefly, 7–9 µm thickness paraffin sections were cut, deparafinized, rehydrated using ethanol gradients and placed in PBS.…”

Section: Methodsmentioning

confidence: 99%

3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats

Ribot

Tournier

Aid‐Launais

et al. 2017

Sci Rep

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Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femoral bone defects in rats. For this, three different biomaterials based on Pullulan-Dextran and containing either Fucoidan or HydroxyApatite or both were implanted. In vivo MRI, ex vivo micro-CT and histology were performed 1, 3 and 5 weeks after implantation. The high spatially resolved (156 × 182 × 195 µm) anatomical images showed a high contrast from the defects filled with biomaterials that decreased over time due to bone formation. The 3D Dynamic Contrast Enhanced (DCE) imaging with high temporal resolution (1 image/19 s) enabled to detect a modification in the Area-Under-The-Gadolinium-Curve over the weeks post implantation. The high sensitivity of MRI enabled to distinguish which biomaterial was the least efficient for bone regeneration, which was confirmed by micro-CT images and by a lower vessel density observed by histology. In conclusion, the methodology developed here highlights the efficiency of longitudinal MRI for tissue engineering as a routine small animal exam.

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The proangiogenic potential of a novel calcium releasing biomaterial: Impact on cell recruitment

Cited by 42 publications

References 45 publications

Characterization of printed PLA scaffolds for bone tissue engineering

Characterization of printed PLA scaffolds for bone tissue engineering

Influence of dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells

3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats

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