The probable protective Effect of Quercetin Against Doxorubicin-Induced Hepatotoxicity in Adult Albino Rats: A Biochemical and Immunohistopathological Study

Abstract

Background: Doxorubicin (DOX) is an anthracycline antineoplastic which causes hepatotoxicity by induction of free radicals and inflammatory liver tissues. Quercetin (QCT) is considered as a potent antioxidant that may ameliorates hepatotoxicity. Aim of the work: The current study was designed to detect the toxic effects of DOX in liver cells and evaluating the possible protective role of QCT against those toxic alterations in adult male albino rats. Materials and methods: forty (40) male albino rates were divided to four groups: Control (group I), DOX-treated (group II): Rats received 18 mg/kg of body weight of DOX. Group III, received 60 ml/kg of QCT combined with DOX. Group IV treated with DOX plus 100 ml of QCT/kg. After scarification of rats, liver enzymes and the bile levels were estimated. The livers were extracted and used in oxidative stress markers, histological and immunological studies. Results: Doxorubicin administration induced an increase in liver enzymes, bile level and oxidative stress markers. QCT administration induced an improvement in those levels, especially with the high dose. The microscopic examinations of the DOX-treated sections showed loss of normal architectures of hepatic cells, enlargement and congestion of the central and portal veins and blood sinusoids, vacuolar degeneration, cholelithiasis and necrosis of H&E stained hepatocytes. PAS-stained liver sections in the DOX-treated group showed an increase in the depth of the stain. Quercetin administration improved these changes and hepatocytes which stained with PAS showed moderate to mild staining, especially with the high dose of QCT. Sections of the stained liver caspase-3, in DOX-treated group showed positive results with intensity. QCT treated groups showed little effects of caspase-3, especially with the high dose. Conclusion: Doxorubicin had toxic effects on rats' livers, with significant improvement after treatment with QCT as an antioxidant substance, and its high doses were more protective than low doses.

Prospective Affirmative Therapeutics of Cannabidiol Oil Mitigates Doxorubicin-Induced Abnormalities in Kidney Function, Inflammation, and Renal Tissue Changes.

“…Reliable indications of kidney impairment include blood urea nitrogen (BUN), albumin, and Creatinine (Ali et al 2021). This work aimed to assess the anti-in ammatory and tubules hydropic degeneration improvement effects of Cannabidiol oil to that of the medication of Trimetazidine in a rat model of Doxorubicin (DOX) -induced renal toxicity.…”

“…Normal renal tissue does not permit albumin to ow out from the bloodstream to the urine, while also ltering creatinine and BUN from the bloodstream into the urine. However, in the event of renal dysfunction, these processes are disturbed: albumin is excreted in the urine, resulting in reduced serum concentrations, and creatinine and BUN are not ltered correctly, resulting in elevated serum values (Ali et al 2021). In our current work, Dox treatment caused increases in serum creatinine and BUN alongside a signi cant decrease in albumin.…”