The Problem of False Coliform Counts on Fruit Ice Cream1

Barber, F. W.; Fram, Harvey

doi:10.4315/0022-2747-18.4.88

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Fig 2 E Coli Colonies On a 1984 Grid-cell Hgmf Photograp1

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1957

1997

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Cited by 6 publications

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“…The possibility of false-positive coliform reactions in pour plates prepared from ice cream has been noted for many years (2,3,8). Stan-dard Methods for the Examination of Dairy Products (7), for example, recommends that "because of the presence of sucrose in ice cream, coliform-like colonies on VRB agar should be confirmed."…”

Section: Fig 2 E Coli Colonies On a 1984 Grid-cell Hgmf Photograpmentioning

confidence: 99%

Improved detection of coliforms and Escherichia coli in foods by a membrane filter method

Sharpe¹,

Peterkin²,

Malik³

1979

Appl Environ Microbiol

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Analytical procedures based on filtration of homogenates through membrane filters, and particularly hydrophobic grid-membrane filters (HGMF), offer definite improvements in the enumeration of Escherichia coli and coliforms in foods. Whereas the counted specimen in pour plates may not usually be greater than 0.1 g, up to 1.0 g of ground beef, green beans, potato, cod, strawberries, or grapes could be filtered and counted on HGMF. Greatly improved limit of detection, reduced interference by noncoliforms, and complete removal of growth inhibitors such as polyphenols were demonstratedfor HGMF, using violet red bile and mFC agars. In addition, counting on HGMF eliminated a false-positive reaction caused by sucrose in ice cream.

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Section: Fig 2 E Coli Colonies On a 1984 Grid-cell Hgmf Photograpmentioning

confidence: 99%