The procedure of polymerase chain reaction — Restriction fragment — Single strand conformation polymorphism analysis by Hha I/Hinc II to detect mitochondrial DNA mutations

Rapid Screening of Fluoroquinolone Resistance Determinants in Streptococcus pneumoniae by PCR-Restriction Fragment Length Polymorphism and Single-Strand Conformational Polymorphism

“…PCR-RF-SSCP has been described as a useful tool for mass screening for DNA polymorphism analysis on other genes (15,26) and is able to detect a nucleotide substitution, deletion, or insertion in up to a 22,000-base-pair amplicon (29). We applied this method for the analysis of the QRDRs of the gyrA, gyrB, parC, and parE genes of S. pneumoniae.…”

Application of PCR-RF-SSCP to study major histocompatibility class II B polymorphism in common carp (Cyprinus carpio L.)

“…For our studies we chose to first cleave our 350 bp PCR fragment by enzyme restriction into two shorter fragments, and then perform a SSCP analysis. This combined method is named polymerase chain reaction-restriction fragments-single strand conformation polymorphism analysis (PCR-RF-SSCP) [33] and has been used for the analysis of human mitochondrial DNA mutations [34], DNA polymorphism studies on the parasite Trichinella [35] and for studies on mutations detection in rice waxy mutants [36].…”

Application of PCR-RFLP analysis on species identification of canned tuna

“…Many techniques derived from PCR were developed to analyze DNA sequences, including random ampliWed polymorphic DNA (RAPD), PCR single stranded conformational polymorphism (PCR-SSCP), and PCR restriction fragment length polymorphism (PCR-RFLP) (Jones, 1991;Williams, Kubelik, Livak, Rafalski, & Tingey, 1990). Among them, PCR-RFLP technique was the most popular to apply in biology, medicine and food science (Kurihara, Tawata, Ikegishi, Aida, & Onaya, 1999;Russell et al, 2000;Sato et al, 1998). In our previous papers, the PCR-RFLP technique has been utilized to identify some species of raw Wsh and processed foods (Hsieh, Chai, & Hwang, 2005;Hsieh, Shiu, Cheng, Chen, & Hwang, 2002;Hwang, Jen, Hsieh, & Shiau, 2004;Lin, Shiau, & Hwang, 2005).…”