The Process of Apoptosis in Follicular Epithelial Cells in the Rabbit Ovary, with Special Reference to Involvement by Macrophages.

Kasuya, Kiyoshi

doi:10.1679/aohc.58.257

Cited by 30 publications

(20 citation statements)

References 8 publications

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“…The large, round, acid phosphatase positive cells found intermingled with GCs were identified as macrophages in accordance with similar observations in the rabbit ovary (Kasuya, 1995).…”

Section: Discussionsupporting

confidence: 90%

Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica)

D’Herde¹,

Prest²,

Roels³

1996

Reprod. Nutr. Dev.

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“…The large, round, acid phosphatase positive cells found intermingled with GCs were identified as macrophages in accordance with similar observations in the rabbit ovary (Kasuya, 1995).…”

Section: Discussionsupporting

confidence: 90%

Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica)

D’Herde¹,

Prest²,

Roels³

1996

Reprod. Nutr. Dev.

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“…Apoptotic granulosa cells were finally recognized and phagocytosed by adjacent granulosa cells at the early stage of atresia or by phagocytes, probably macrophages, which invade the follicle from the outside the tissue at the advanced stage of atresia [42]. In general, it appears that phagocytes taking up apoptotic cells may employ one or more of a range of possible recognition mechanisms, while in turn apoptotic cells may display their 'edible' status in a number of different ways [20].…”

Section: Discussionmentioning

confidence: 99%

Examination of Granulosa Cell Glycoconjugates Which Change During Follicular Atresia in the Pig Ovary

Kimura

Manabe

Matsushita

et al. 1998

Journal of Reproduction and Development

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Abstract. Changes of glycoconjugates in granulosa cells during follicular atresia were examined by lectin histochemical and biochemical techniques. Twenty-four lectins were used to visualize the different glycoconjugates of granulosa cells in frozen sections of the pig ovary. Four lectins showed stronger staining of the granulosa cells in atretic than in healthy follicles. Very strong and/or strong staining with Sambucus sieboldiana agglutinin (SSA) was seen in scattered granulosa cells of atretic follicles, but those of healthy follicles were not stained with this lectin. In atretic follicles, very strong-moderate diffuse staining was seen with Aleuria aurantia lectin (AAL), Pisum sativum agglutinin (PSA) and Lens culinaris agglutinin (LCA) in granulosa cells, in contrast with very weak staining in those of healthy follicles. Furthermore, lectin blot analysis with three lectins (SSA, AAL, and PSA) indicated several glycoproteins with increased staining in atretic relative to healthy follicles for these three lectins. Especially, two SSA-positive glycoproteins (62 and 64 kD) of granulosa cells in atretic follicles were specific and characteristic which were not detected in those in healthy follicles. These results suggest that changes of some glycoconjugates in granulosa cells may be involved in granulosa cell apoptosis and follicular atresia.

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“…This process of atresia is currently regarded as being due to apoptosis, a genetically programmed cell death, of the granulosa cell mass and ultimately of the oocyte (Hughes & Gorospe, 1991 ;Tilly et al 1991). Several authors have claimed that macrophages are active in phagocytosing apoptotic (pyknotic) granulosa cells in atretic follicles in mice (Byskov, 1974) and other animals (Peluso et al 1980 ;Bukovsky et al 1993Bukovsky et al , 1995Kasuya, 1995Kasuya, , 1997.…”

Section: mentioning

confidence: 99%

“…Bukovsky et al identified macrophages immunohistochemically in rat (1993) andhuman (1995) ovaries. Kasuya (1995) reported that apoptotic granulosa cells were phagocytosed by ' large round cells ', which were identified as macrophages on the basis of acid phosphatase positivity in rabbits and positive immunoreactivity with an antimacrophage monoclonal antibody (MR-1) in guinea pigs (Kasuya, 1997).…”

Section: mentioning

confidence: 99%

Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice

et al. 2000

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We examined numerous ovarian follicles from 32-35 d virgin mice by transmission electron microscopy and light microscopic immunohistochemistry. No macrophages were seen, but various stages of apoptotic granulosa cells were encountered. Presumably a granulosa cell or its debris in an advanced stage of apoptosis was destined to be phagocytosed by adjacent normal-looking granulosa cells. Other granulosa cells of normal appearance were seen in the region of the zona pellucida in contact with and apparently phagocytosing atrophic oocytes. Such granulosa cells were characterised by the presence of gap junctions with other cells and frequently contained annular gap junctions in the cytoplasm. To confirm the lack of involvement of macrophages in the process of follicular atresia and elimination, specially prepared ovarian sections were incubated with antimouse macrophage monoclonal antibodies (F4\80, Mac-1, Mac-2). None of the follicles examined showed positive immunoreactivity with these antibodies. Atretic follicles may shrink and eventually disappear from the ovary as a result of repeated apoptosis and phagocytosis by granulosa cells. There is no evidence for the presence or involvement of macrophages in the atretic follicles, at least in prereproductive mice as examined.

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The Process of Apoptosis in Follicular Epithelial Cells in the Rabbit Ovary, with Special Reference to Involvement by Macrophages.

Cited by 30 publications

References 8 publications

Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica)

Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica)

Examination of Granulosa Cell Glycoconjugates Which Change During Follicular Atresia in the Pig Ovary

Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice

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