The process of granule exocytosis in non-stimulated atrial granular cells of the snail, Achatina achatina: An ultrastructural, histochemical and immunocytochemical study

Bystrova, Olga V.; Shabelnikov, Sergey; Martynova, Marina G.

doi:10.1016/j.acthis.2013.04.014

Cited by 7 publications

(8 citation statements)

References 27 publications

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“…A striking similarity between the snail atrial GCs and vertebrate mast cells in morphological features, the proteins their granules contain, the tight contacts they form with nerve endings, was already discussed in our other articles (Shabelnikov et al 2009;Bystrova et al 2013). Here we would like to mention the capacity of mast cells for recycling after complete degranulation (Burwen 1982;Nielsen & Clausen 1982).…”

Section: Discussionmentioning

confidence: 78%

“…As we have shown earlier, granules of the atrial GCs contain natriuretic peptide ANP (Martynova et al 2004), Hsp70 (Martynova et al 2007, substance P andFMRFamide (Shabelnikov et al 2008), histamine and serotonin (Bystrova et al 2013). All these peptides possess a wide spectrum of functions and are implicated in a broad range of physiological processes.…”

Section: Discussionmentioning

confidence: 87%

“…Abundant large GCs are located on the surface of muscles under endothelial cells and contact tightly with nerve endings. In previous studies, we have shown that the granules of these cells contain wide variety of bioactive substances (Martynova et al 2004(Martynova et al , 2007Shabelnikov et al 2008;Bystrova et al 2013), are very sensitive and answer with degranulation to different kind of stress-induced factors such as electrical stimulation of the heart nerve (Shabelnikov et al 2009) or the injection of some neuromediators (our unpublished data). The high stressreactivity of the atrial GCs and their functional analogy to vertebrate mast cells has determined our choice of snail atrium as an object of study.…”

Section: Introductionmentioning

confidence: 94%

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Long-term consequences of a short-term hypergravity load in a snail model

Martynova

Shabelnikov

Bystrova

2014

International Journal of Astrobiology

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Here we focused on the dynamic processes in the snail at different time after short-term hypergravity load (STHL) by monitoring the state of neuroendocrine and immune systems, the nucleic acid synthesis levels in the atrial cells, and the behaviour of the atrial granular cells (GCs). We observed that immediately after centrifugation (14 g for 15 min) in the snail haemolymph concentration of dopamine and noradrenaline (measured by high-performance liquid chromatography) and the number of circulating haemocytes and their proliferative activity (estimated by the direct cell counting and [ 3 H]thymidine incorporation, respectively) increased significantly, whereas the concentration of adrenaline decreased. Twenty-four hours after STHL, the levels of catecholamines and haemocytes returned to their control values. In the atrial epicardial and endothelial cells, a notable drop of transcription activity (evaluated by [ 3 H]uridine autoradiography) from the baseline in the immediate post-STHL period was followed by its gradual increase reaching a maximum at the day 5 and subsequent decrease to control value by the day 10. In endothelial cells, DNA-synthesizing activity (evaluated by [ 3 H]thymidine autoradiography) equal to zero before and just after STHL, increased significantly at the day 5, and decreased by the day 10. The atrial GCs underwent total degranulation. Formed as a result small ungranulated cells exhibited DNA synthesis. Afterwards, most probably, the GCs divided and regranulated. One month after STHL the GC population had been restored. Overall, STHL has triggered an immediate reaction of the neuroendocrine and immune systems and initiated long-lasting processes at a cellular level, which included alterations in activity of nucleic acid syntheses in the epicardial and endothelial cells and remodelling of the GC population in the atrium.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 94%

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Long-term consequences of a short-term hypergravity load in a snail model

Martynova

Shabelnikov

Bystrova

2014

International Journal of Astrobiology

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“…The visually perceived stimuli are rapidly processed by CNS, and the signal is transmitted into the heart. Responsible neurotransmitters are stored and released by the corresponding neurons of CNS, but there are also substances that are present in cardiac and pericardial tissues, for example, in the granule cells of the atrium (Bystrova, Shabelnikov, & Martynova, ). However, it is not clear what the trigger for these cardiac secretory cells is, when the release occurs, and whether or not they also respond during “fight or flight” behavior.…”

Section: Introductionmentioning

confidence: 99%

Limulus and heart rhythm

et al. 2018

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Great interest in the comparative physiology of hearts and their functions in Animalia has emerged with classic papers on Limulus polyphemus and mollusks. The recurrent cardiac activity-heart rate-is the most important physiological parameter and when present the kardia (Greek) is vital to the development of entire organs of the organisms in the animal kingdom. Extensive studies devoted to the regulation of cardiac rhythm in invertebrates have revealed that the basics of heart physiology are comparable to mammals. The hearts of invertebrates also beat spontaneously and are supplied with regulatory nerves: either excitatory or inhibitory or both. The distinct nerves and the source of excitation/inhibition at the level of single neurons are described for many invertebrate genera. The vertebrates and a majority of invertebrates have myogenic hearts, whereas the horseshoe crab L. polyphemus and a few other animals have a neurogenic cardiac rhythm. Nevertheless, the myogenic nature of heartbeat is precursor, because the contraction of native and stem-cellderived cardiomyocytes does occur in the absence of any neural elements. Even in L. polyphemus, the heart rhythm is myogenic at embryonic stages.

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“…pomatia [ 8 ], H . aspersa , Strophocheilus oblongus [ 9 ], Achatina fulica [ 10 ] and Achatina achatina [ 11 ]. The secretory granules of granular cells have been immunostained with antibodies against atrial natriuretic peptide [ 12 ], Hsp70 [ 10 ], substance P and FMRFamide [ 13 ], and serotonin and histamine [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Cysteine-Rich Atrial Secretory Protein from the Snail Achatina achatina: Purification and Structural Characterization

Shabelnikov

Kiselev

2015

PLoS ONE

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Despite extensive studies of cardiac bioactive peptides and their functions in molluscs, soluble proteins expressed in the heart and secreted into the circulation have not yet been reported. In this study, we describe an 18.1-kDa, cysteine-rich atrial secretory protein (CRASP) isolated from the terrestrial snail Achatina achatina that has no detectable sequence similarity to any known protein or nucleotide sequence. CRASP is an acidic, 158-residue, N-glycosylated protein composed of eight alpha-helical segments stabilized with five disulphide bonds. A combination of fold recognition algorithms and ab initio folding predicted that CRASP adopts an all-alpha, right-handed superhelical fold. CRASP is most strongly expressed in the atrium in secretory atrial granular cells, and substantial amounts of CRASP are released from the heart upon nerve stimulation. CRASP is detected in the haemolymph of intact animals at nanomolar concentrations. CRASP is the first secretory protein expressed in molluscan atrium to be reported. We propose that CRASP is an example of a taxonomically restricted gene that might be responsible for adaptations specific for terrestrial pulmonates.

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The process of granule exocytosis in non-stimulated atrial granular cells of the snail, Achatina achatina: An ultrastructural, histochemical and immunocytochemical study

Cited by 7 publications

References 27 publications

Long-term consequences of a short-term hypergravity load in a snail model

Long-term consequences of a short-term hypergravity load in a snail model

Limulus and heart rhythm

Cysteine-Rich Atrial Secretory Protein from the Snail Achatina achatina: Purification and Structural Characterization

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