The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.

Zerial, Marino; Toschi, Luisella; Ryseck, Rolf-Peter; Schuermann, Marcus; Müller, Rolf; Bravo, Rodrigo

doi:10.1002/j.1460-2075.1989.tb03441.x

Cited by 529 publications

(256 citation statements)

References 42 publications

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“…Yeast expression vectors used for titration of two-hybrid interaction activity are pGBT9 and pGADGH (Clontech). The full length or truncated cDNAs of human USF, c-fos, fra1 and c-jun as well as mouse fra2 (Foletta et al, 1994) and fosB (Zerial et al, 1989) were cloned in these vectors. For in vitro translation, the inserts were subcloned into pBluescriptSK-with appropriate restriction enzymes.…”

Section: Plasmid Constructionsmentioning

confidence: 99%

Cross-family interaction between the bHLHZip USF and bZip Fra1 proteins results in down-regulation of AP1 activity

Pognonec¹,

Boulukos²,

Aperlo³

et al. 1997

Oncogene

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Heterodimerization among the basic-leucine zipper (bZIP) proteins or among the basic-helix ± loop ± helixleucine zipper (bHLHZip) proteins confers a multitude of combinational activities to these transcription factors. To further examine the function of the bHLHZip protein, USF, we screened for cellular proteins which could directly interact with USF using the yeast two-hybrid system. A bZip protein, Fra1, was found to e ciently interact with USF. USF speci®cally interacts with Fra1 but not with other closely related family members, c-Fos, Fra2, FosB, or with c-Jun. Both the bHLHZip and the N-terminal regions of Fra1 are required for e cient interaction with USF. In vivo association between USF and Fra1 has been demonstrated by co-immunoprecipitation. Expression of exogenous USF led to a decrease in AP1-dependent transcription in F9 cells. Co-expression of exogenous Fra1 restored the AP1 activity in a dosedependent manner. These data show that USF and Fra1 physically and functionally interact demonstrating that cross-talk occurs between factors of distantly related transcription families.

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Section: Plasmid Constructionsmentioning

confidence: 99%

Cross-family interaction between the bHLHZip USF and bZip Fra1 proteins results in down-regulation of AP1 activity

Pognonec¹,

Boulukos²,

Aperlo³

et al. 1997

Oncogene

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“…Since their discovery in the late 1980s, cellular protooncogenes (Nishina et al, 1990;Sagar et al, 1988;Zerial et al, 1989) have become widely utilized tools to examine the effect of an acute stimulus; pharmacological or sensory (Beckmann and Wilce, 1997;Herdegen and Leah, 1998;Ziolkowska and Przewlocki, 2002). These transcription factors are also referred to as immediate-early genes (IEG) (Herrera and Robertson, 1996) and can be detected using immunohistochemistry (Dragunow and Faull, 1989) or in situ hybridization (Ziolkowska and Przewlocki, 2002) with members of the Fos-and Early growth response (Egr)-families being among the most extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Alterations in c-fos and Egr-1 (zif268) Expression Throughout the Rat Brain Following Acute Administration of Different Classes of Antidepressant Compounds

Slattery

Morrow²,

Hudson

et al. 2005

Neuropsychopharmacol

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The majority of immediate-early gene (IEG) studies focus on a few key brain regions associated with the class of psychoactive compound being studied. Recently, using a meta-analysis of the c-fos literature, we demonstrated the utility of c-fos profiling to classify such compounds. The present study examined acute delivery of a range of antidepressant classes; fluoxetine, imipramine, LiCl, and mirtazapine. The dual aims were to study the IEG profiles of these varying classes of antidepressants throughout the rat brain and to compare the utility of c-fos or Egr-1 as IEGs to classify clinically efficacious antidepressants. All antidepressants increased c-fos mRNA in the central amygdala, as previously shown, while c-fos was also increased in the anterior insular cortex and significantly decreased within the septum. Although acute antidepressant administration altered c-fos expression in a number of brain regions, Egr-1 expression was only significantly altered in the central amygdala, suggesting that Egr-1 may not be as useful a marker to investigate acute antidepressant treatment. The fact that these drugs, including the previously unclassified antidepressant mirtazapine, share a number of common loci of activation, which are implicated by human and animal studies in depression, adds further support to the use of IEG mapping to classify psychoactive compounds.

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“…The jun and fos families of genes are composed of three jun members (c-jun, junB, and junD) (Bohmann et al, 1987;Hirai et al, 1989;Maki et al, 1987;Ryder et al, , 1989 and four di erent fos partners (c-fos, fosB, fra1 and fra2) (Cohen and Curran, 1988;Nishina et al, 1990;Zerial et al, 1989). These genes encode components of the AP1 transcription factor formed by homo-or hetero-dimerization of the Jun and Fos proteins.…”

Section: Introductionmentioning

confidence: 99%

Transformation by ras modifies AP1 composition and activity

et al. 1997

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The Ras proteins play a central role in regulating cell growth and their mutation can lead to abnormal proliferation. To analyse the potential link betwen AP1 activity, encoded by members of the jun and fos gene families, and Ras-mediated cellular transformation, we have studied several NIH3T3 clones which overexpress the Ha-Ras or Ki-Ras oncogenes. These transformed ®broblasts accumulated higher levels of cJun, JunB, Fra1 and Fra2 proteins relative to their normal counterparts. They also displayed increased AP1 DNA binding activity which was predominantly composed of cJun and Fra1 containing dimers. Following serum stimulation of Ras clones, the elevated levels of cJun and Fra1 remained steady, while the induction of JunB and Fra2 was partially attenuated. Moreover, deregulated Ras signaling resulted in a complete loss of the serum inducibility of cFos and FosB. Ectopic co-expression of cJun and Fra1 in NIH3T3 ®broblasts led to a transformed phenotype, attenuation of cFos serum inducibility, increased AP1 activity and Cyclin D1 accumulation, all characteristics of oncogenic Ras expressing cells. These results demonstrate that cJun and Fra1 are crucial mediators of the Ras-transformation process.

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The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.

Cited by 529 publications

References 42 publications

Cross-family interaction between the bHLHZip USF and bZip Fra1 proteins results in down-regulation of AP1 activity

Cross-family interaction between the bHLHZip USF and bZip Fra1 proteins results in down-regulation of AP1 activity

Comparison of Alterations in c-fos and Egr-1 (zif268) Expression Throughout the Rat Brain Following Acute Administration of Different Classes of Antidepressant Compounds

Transformation by ras modifies AP1 composition and activity

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