The product of fem-1, a nematode sex-determining gene, contains a motif found in cell cycle control proteins and receptors for cell-cell interactions

Spence, Andrew M.; Coulson, Alan; Hodgkin, Jonathan

doi:10.1016/0092-8674(90)90346-g

Cited by 172 publications

(110 citation statements)

References 42 publications

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“…Ankyrins are well-known adapter proteins that mediate protein-protein interaction through the ANK repeat motif. Some ankyrin-repeat-containing genes are essential for the process of sex determination, such as ankyrin 6 in Arabidopsis thaliana (Yu et al, 2010), fem-1 in C. elegans (Spence et al, 1990) and ankyrin pk2 in Wolbachia pipientis (Pichon et al, 2012). Ankyrin 6 in A. thaliana has a central role in male-female gamete recognition, possibly by regulating mitochondrial gene expression.…”

Section: Discussionmentioning

confidence: 99%

High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis

et al. 2015

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The sex determination system in crabs is believed to be XY-XX from karyotypy, but centromeres could not be identified in some chromosomes and their morphology is not completely clear. Using quantitative trait locus mapping of the gender phenotype, we revealed a ZW-ZZ sex determination system in Eriocheir sinensis and presented a high-density linkage map covering~98.5% of the genome, with 73 linkage groups corresponding to the haploid chromosome number. All sex-linked markers in the family we used were located on a single linkage group, LG60, and sex linkage was confirmed by genome-wide association studies (GWAS). Forty-six markers detected by GWAS were heterozygous and segregated only in the female parent. The female LG60 was thus the putative W chromosome, with the homologous male LG60 as the Z chromosome. The putative Z and W sex chromosomes were identical in size and carried many homologous loci. Sex ratio (5:1) skewing towards females in induced triploids using unrelated animals also supported a ZW-ZZ system. Transcriptome data were used to search for candidate sex-determining loci, but only one LG60 gene was identified as an ankyrin-2 gene. Double sex-and mab3-related transcription factor 1 (Dmrt1), a Z-linked gene in birds, was located on a putative autosome. With complete genome sequencing and transcriptomic data, more genes on putative sex chromosomes will be characterised, thus leading towards a comprehensive understanding of the sex determination and differentiation mechanisms of E. sinensis, and decapod crustaceans in general.

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Section: Discussionmentioning

confidence: 99%

High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis

et al. 2015

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“…Residues identical in at least three of the six repeats are boxed and appear in the glp-1 consensus. The consensus sequences derived from the cdclO/SWI6 repeats of fem-1 (Spence et al, 1990) and ankyrin (Lux et al, 1990) are shown for comparison. (B) Alignment of the SWI6-4 repeats of glp-1 and lin-12, with emphasis on the region with mutations.…”

Section: Molecular Basis Of the Putative Null Glp-1(if) Mutationsmentioning

confidence: 99%

“…The temperature sensitivity of the mutations mapping to the cdclO/SWI6 repeats of glp-1 is consistent with this hypothesis. Many cdclO/SWI6 repeat-containing proteins bind and/or regulate a putative transcription factor: SW14 and SWI6 of S. cerevisae (Andrews and Herskowitz, 1989a,b), fem-1 of C. elegans (Spence et al, 1990), cactus of Drosophila (Nusslein-Volhard, personal communication), IkB (Haskill et al, 1991) and the 11 subunit of GABP from mammals (Thompson et al, 1991). The exceptions are ankyrin and a-latrotoxin, which are also exceptional in their high number of repeats (Kiyatkin et al, 1990;Lux et al, 1990).…”

Section: Individual Egf-like Repeats May Have Distinct Functionsmentioning

confidence: 99%

Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans.

Kodoyianni

Maine

Kimble

1992

MBoC

143

126

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The glp-1 gene encodes a membrane protein required for inductive cell interactions during development of the nematode Caenorhabditis elegans. Here we report the molecular characterization of 15 loss-of-function (If) mutations of glp-1. Two nonsense mutations appear to eliminate glp-i activity; both truncate the glp-1 protein in its extracellular domain and have a strong loss-of-function phenotype. Twelve missense mutations and one in-frame deletion map to sites within the repeated motifs of the glp-1 protein (10 epidermal growth factor [EGF]-like and 3 LNG repeats extracellularly and 6 cdclO/SWI6, or ankyrin, repeats intracellularly). We find that all three types of repeated motifs are critical to glp-I function, and two individual EGF-like repeats may have distinct functions. Intriguingly, all four missense mutations in one phenotypic class map to the N-terminal EGF-like repeats and all six missense mutations in a second phenotypic class reside in the intracellular cdclO/ SWI6 repeats. These two clusters of mutations may identify functional domains within the glp-1 protein.

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“…pRF4 contains rol-6 (su1006), which confers a dominant rolling phenotype to transformed animals allowing them to be easily identified (Mello et al, 1991). The plasmid DNA used in transformation was prepared by two methods: 1) alkaline lysis (Sambrook et al, 1989) followed by a further lithium chloride purification procedure (Spence et al, 1990) for the large pools, and 2) plasmid mini preparation using QIAGEN tip-20s (Hybaid) for the smaller pools and individual plasmids. The second method appeared to give a higher number of transformed animals when injected into C .…”

Section: Experimental Procedures Maintenance and Transformation Of Cmentioning

confidence: 99%

Molecular markers of differentiation in Caenorhabditis elegans obtained by promoter trapping

Young

Hope

1993

Developmental Dynamics

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Differentiation of specific cell types during animal development can be detected by monitoring expression of appropriate genes. For this study, six different P-galactosidase expression patterns which can be used as differentiation markers in the nematode Caenorhabditis elegans are described. An earlier promoter trap screen identified pools of recombinant plasmids which gave patterns of P-galactosidase expression when used to transform C. elegans. Each recombinant plasmid contained a random fragment of C. elegans genomic DNA fused upstream of a promoterless lac2 gene. Six of these pools were chosen, and individual pattern-producing plasmids within these pools were identified. The expression patterns have been characterized more thoroughly than in the original screen, thereby providing molecular markers for differentiation of several cell types. Many of the expression patterns involve more than one cell type. The genomic origin of the inserts of active plasmids were determined through localization on the physical genome map. 0 1993 Wiley-Liss, Inc.

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The product of fem-1, a nematode sex-determining gene, contains a motif found in cell cycle control proteins and receptors for cell-cell interactions

Cited by 172 publications

References 42 publications

High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis

High-density linkage mapping aided by transcriptomics documents ZW sex determination system in the Chinese mitten crab Eriocheir sinensis

Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans.

Molecular markers of differentiation in Caenorhabditis elegans obtained by promoter trapping

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