The production and application of bacterial exopolysaccharides as biomaterials for bone regeneration

Bagnol, Romain; Grijpma, Dirk W.; Eglin, David; Moriarty, T. Fintan

doi:10.1016/j.carbpol.2022.119550

Cited by 23 publications

(9 citation statements)

References 173 publications

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“…Moreover, certain types of bacterial EPS have been shown to boost angiogenesis, which is vital for the restoration and regeneration of tissues via the creation of new blood vessels [324] . Additionally, bacterial EPS have been studied for their possible application in tissue engineering as they can serve as supportive structures to promote cell growth and differentiation [325,326] .…”

Section: Wound Healing and Tissue Engineeringmentioning

confidence: 99%

Bacterial Exopolysaccharides: From Production to Functional Features

Talbi,

Elmarrakchy,

Youssfi

et al. 2023

Prog Micobes Mol Biol

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Exopolysaccharides, known as bacterial EPS, are complex sugar polymers that bacteria secrete into their environment. EPSs play a crucial role in bacterial survival and proliferation by protecting cells from environmental threats. They also contribute to proper adhesion to numerous surfaces through biofilm production. Bacterial EPS display a wide range of biological activities conferred by their outstanding physical and chemical properties, which make them great candidates for medical and industrial applications. The main biological activities recorded up-to-day include antioxidant, anticancer, antiviral, anti-inflammatory, anticancer, antibacterial, immune-modulatory and chelating properties. They also can be used as thickening agents in the industry or as additives to improve soil quality in agriculture. The current review offers a thorough overview of bacterial EPS, their biosynthesis processes and regulation, and biotechnological strategies to increase their production. The review also unravels the main extraction, purification, and identification techniques and highlights the key functional features of these complex molecules.

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Section: Wound Healing and Tissue Engineeringmentioning

confidence: 99%

Bacterial Exopolysaccharides: From Production to Functional Features

Talbi,

Elmarrakchy,

Youssfi

et al. 2023

Prog Micobes Mol Biol

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“…In particular, the production of natural polymers by microorganisms has gained great attention among researchers due to a series of advantages over their plant- and animal-based counterparts . Some advantages are that their production is less climate- or seasonal-dependent, and they present a smaller footprint and a higher growth rate. , Natural polymers produced by microorganisms also have a higher structural diversity, which may unravel even more applications. ,, Microbial biopolymers can be found intracellularly (e.g., polyphosphate and polyhydroxyalkanoates) or secreted by the cells as in the case of many polysaccharides, proteins, and glycoproteins. , The latter type of biopolymers is known as extracellular polymeric substances (EPS) …”

Section: Introductionmentioning

confidence: 99%

Fractionation of Extracellular Polymeric Substances by Aqueous Three-Phase Partitioning Systems

Antunes,

Cintra,

Bredel

et al. 2024

Ind. Eng. Chem. Res.

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Extracellular polymeric substances (EPS) are natural polymers secreted by microorganisms and represent a key chemical for the development of a range of circular economy applications. The production of EPS comes with notable challenges such as downstream processing. In this work, a three-phase partitioning (TPP) system was investigated as a fractionation technique for the separation of polysaccharides and proteins, both present in the EPS culture broth. The effect of the type of phase-forming compounds (alcohol, polymer, or ionic liquid, in combination with salt) and its concentration were evaluated and compared to the results previously obtained with model systems. The recyclability of phase-forming compounds used to form the fractionation platform was assessed by ultrafiltration. The best fractionation of EPS was achieved using a TPP system composed of 23 wt % ethanol and 25% K 3 C 6 H 5 O 7 as 82% EPS-PS partitioned to the salt-rich/bottom phase, and 76% EPS-PN was recovered as an interfacial precipitate, which could be readily resolubilized in water. This represented an increase of 1.24 and 2.83-fold in the purity of EPS-PS and EPS-PN, respectively, in relation to the initial feed concentration. Finally, high recovery yields of phase-forming compounds (>99%) and fractionated EPS (>80%) were obtained using ultrafiltration/diafiltration (UF/DF) as the regeneration technique. The substantial fractionation yields, selectivity, and recyclability of the phase-forming compounds confirm the potential of TPP systems in combination with UF/DF as the separation method for real biopolymer mixtures. Key contributions of this study include the demonstration of the applicability of a readily scalable and cost-effective separation technique for the fractionation of EPS from real EPS-containing broths, while also the limitations of prescreening with model systems became clear through the observed deviating trends between model system studies and real broth studies.

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“…The most widely used bacterial EPS in biomedical applications include cellulose, 5 which is used due to its excellent mechanical properties and high water retention capabilities, alginate 6 due to the facile cross-linking potential, and xanthan gum due to its excellent water solubility, biocompatibility, immune inertness, and positive viscoelastic properties. 7 Such bacterial EPS have been used in a number of biomedical engineering applications, including for bone regeneration 8 and tissue engineering 9 as well as in wound healing 5 and osteoarthritis. 7 In many cases, the EPS may serve as a rather inert, biocompatible component in a composite biomaterial that may be loaded with other active agents.…”

Section: ■ Introductionmentioning

confidence: 99%

Physicochemical Characterization and Immunomodulatory Activity of Polyelectrolyte Multilayer Coatings Incorporating an Exopolysaccharide from Bifidobacterium longum

Bagnol,

Siverino,

Barnier

et al. 2023

Biomacromolecules

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The production and application of bacterial exopolysaccharides as biomaterials for bone regeneration

Cited by 23 publications

References 173 publications

Bacterial Exopolysaccharides: From Production to Functional Features

Bacterial Exopolysaccharides: From Production to Functional Features

Fractionation of Extracellular Polymeric Substances by Aqueous Three-Phase Partitioning Systems

Physicochemical Characterization and Immunomodulatory Activity of Polyelectrolyte Multilayer Coatings Incorporating an Exopolysaccharide from Bifidobacterium longum

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