The production and methods of use of polyclonal antisera to the pathogenic organisms <i>Aeromonas salmonicida, Yersinia ruckeri</i> and <i>Renibacterium salmoninarum</i>

Smith, Adrian; Goldring, Owen; Dear, G.

doi:10.1111/j.1095-8649.1987.tb05317.x

Cited by 8 publications

(6 citation statements)

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“…Identification of Y. ruckeri can also be done using serological tests including enzyme-linked immunosorbent assay (ELISA) (Cossarini-Dunier, 1985), immunofluorescence antibody technique (IFAT) (Smith et al, 1987) and agglutination test (Kumar et al, 2015). There is a constant need for more efficient, sensitive, specific and cheaper methods to diagnose Y. ruckeri.…”

Section: Diagnosismentioning

confidence: 99%

The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen

Wróbel

Ottoni

Leo

et al. 2018

Journal of Structural Biology

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Inverse autotransporters comprise the recently identified type Ve secretion system and are exemplified by intimin from enterohaemorrhagic Escherichia coli and invasin from enteropathogenic Yersiniae. These proteins share a common domain architecture and promote bacterial adhesion to host cells. Here, we identified and characterized two putative inverse autotransporter genes in the fish pathogen Yersinia ruckeri NVH_3758, namely yrInv (for Y. ruckeri invasin) and yrIlm (for Y. ruckeri invasin-like molecule). When trying to clone the highly repetitive genes for structural and functional studies, we experienced problems in obtaining PCR products. PCR failures and the highly repetitive nature of inverse autotransporters prompted us to sequence the genome of Y. ruckeri NVH_3758 using PacBio sequencing, which produces some of the longest average read lengths available in the industry at this moment. According to our sequencing data, YrIlm is composed of 2603 amino acids (7812bp) and has a molecular mass of 256.4kDa. Based on the new genome information, we performed PCR analysis on four non-sequenced Y. ruckeri strains as well as the sequenced. Y. ruckeri type strain. We found that the genes are variably present in the strains, and that the length of yrIlm, when present, also varies. In addition, the length of the gene product for all strains, including the type strain, was much longer than expected based on deposited sequences. The internal repeats of the yrInv gene product are highly diverged, but represent the same bacterial immunoglobulin-like domains as in yrIlm. Using qRT-PCR, we found that yrIlm and yrInv are differentially expressed under conditions relevant for pathogenesis. In addition, we compared the genomic context of both genes in the newly sequenced Y. ruckeri strain to all available PacBio-sequenced Y. ruckeri genomes, and found indications of recent events of horizontal gene transfer. Taken together, this study demonstrates and highlights the power of Single Molecule Real-Time technology for sequencing highly repetitive proteins, and sheds light on the genetic events that gave rise to these highly repetitive genes in a commercially important fish pathogen.

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Section: Diagnosismentioning

confidence: 99%

The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen

Wróbel

Ottoni

Leo

et al. 2018

Journal of Structural Biology

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“…While Y. ruckeri can grow at a wide range of temperatures, its thermal optimum is 20–28 °C [ 2 , 4 ]. Y. ruckeri can be detected using ELISA, agglutination and immunofluorescence antibodies [ 33 ]. Molecular detection techniques include restriction fragment length polymorphism [ 34 ], loop-mediated isothermal amplification (LAMP) [ 35 ] and polymerase chain reaction (PCR) [ 32 , 36 , 37 ].…”

Section: Diagnosismentioning

confidence: 99%

Yersinia ruckeri, the causative agent of enteric redmouth disease in fish

Kumar

Menanteau–Ledouble

Saleh

et al. 2015

Vet Res

163

136

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Enteric redmouth disease (ERM) is a serious septicemic bacterial disease of salmonid fish species. It is caused by Yersinia ruckeri, a Gram-negative rod-shaped enterobacterium. It has a wide host range, broad geographical distribution, and causes significant economic losses in the fish aquaculture industry. The disease gets its name from the subcutaneous hemorrhages, it can cause at the corners of the mouth and in gums and tongue. Other clinical signs include exophthalmia, darkening of the skin, splenomegaly and inflammation of the lower intestine with accumulation of thick yellow fluid. The bacterium enters the fish via the secondary gill lamellae and from there it spreads to the blood and internal organs. Y. ruckeri can be detected by conventional biochemical, serological and molecular methods. Its genome is 3.7 Mb with 3406–3530 coding sequences. Several important virulence factors of Y. ruckeri have been discovered, including haemolyin YhlA and metalloprotease Yrp1. Both non-specific and specific immune responses of fish during the course of Y. ruckeri infection have been well characterized. Several methods of vaccination have been developed for controlling both biotype 1 and biotype 2 Y. ruckeri strains in fish. This review summarizes the current state of knowledge regarding enteric redmouth disease and Y. ruckeri: diagnosis, genome, virulence factors, interaction with the host immune responses, and the development of vaccines against this pathogen.

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“…The pathogen may be identified using biochemical characteristics as described above. Based on serological characteristics, other methods may be used in detecting Y. ruckeri such as ELISA, agglutination test and immunofluorescence antibody technique (IFAT) (Smith, Goldring & Dear 1987).…”

Section: The Diseasementioning

confidence: 99%

Yersinia ruckeri infections in salmonid fish

Tobback

Decostere

Hermans

et al. 2007

Journal of Fish Diseases

220

176

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Yersinia ruckeri is the causative agent of yersiniosis or enteric redmouth disease leading to significant economic losses in salmonid aquaculture worldwide. Infection may result in a septicaemic condition with haemorrhages on the body surface and in the internal organs. Despite the significance of the disease, very little information is available on the pathogenesis, hampering the development of preventive measures to efficiently combat this bacterial agent. This review discusses the agent and the disease it causes. The possibility of the presence of similar virulence markers and/or pathogenic mechanisms between the Yersinia species which elicit disease in humans and Y. ruckeri is also examined.

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The production and methods of use of polyclonal antisera to the pathogenic organisms Aeromonas salmonicida, Yersinia ruckeri and Renibacterium salmoninarum

Cited by 8 publications

References 0 publications

The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen

The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen

Yersinia ruckeri, the causative agent of enteric redmouth disease in fish

Yersinia ruckeri infections in salmonid fish

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