The production of C3, PGE2 and TxB2 by splenic, alveolar, and peritoneal guinea pig macrophages

Ogle, Cora K.; Ogle, James D.; Johnson, C.R.; L, Keynton; Alexander, J. Wesley

doi:10.1016/0090-6980(88)90070-6

Cited by 16 publications

(2 citation statements)

References 24 publications

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“…In splenic tissues, mPGES-1 accounts for the majority of basal (COX1-dependent) PGE 2 synthesis, and the in vivo mPGES-1 deletion abolished LPS-inducible PGE 2 synthesis [44]. Normal splenic macrophages produce low levels of PGE 2 when compared with bone-marrow-derived macrophages (BMDM; Table 1), AMs, and peritoneal macrophages [45]. However, high levels of this mediator are produced by splenic macrophages in chronic inflammatory conditions, such as mycobacterial infection [46].…”

Section: Spleenmentioning

confidence: 99%

Prostaglandin and the Suppression of Phagocyte Innate Immune Responses in Different Organs

Medeiros

Peres‐Buzalaf

Verdan

et al. 2012

Mediators of Inflammation

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The local and systemic production of prostaglandin E2 (PGE2) and its actions in phagocytes lead to immunosuppressive conditions. PGE2 is produced at high levels during inflammation, and its suppressive effects are caused by the ligation of the E prostanoid receptors EP2 and EP4, which results in the production of cyclic AMP. However, PGE2 also exhibits immunostimulatory properties due to binding to EP3, which results in decreased cAMP levels. The various guanine nucleotide-binding proteins (G proteins) that are coupled to the different EP receptors account for the pleiotropic roles of PGE2 in different disease states. Here, we discuss the production of PGE2 and the actions of this prostanoid in phagocytes from different tissues, the relative contribution of PGE2 to the modulation of innate immune responses, and the novel therapeutic opportunities that can be used to control inflammatory responses.

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Section: Spleenmentioning

confidence: 99%

Prostaglandin and the Suppression of Phagocyte Innate Immune Responses in Different Organs

Medeiros

Peres‐Buzalaf

Verdan

et al. 2012

Mediators of Inflammation

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“…Our uno‹cial study showed that CPFX was remarkably stable in AMs and ELF for a long time (data not shown). Although AMs produce and secrete various bioactive substances, such as enzymes, cytokines, complements, proteins, lipids and reactive oxygen species, [60][61][62][63][64][65] these bioactive substances may not aŠect the stability of CPFX in AMs and ELF. In addition, it is important that the CPFX administered does not injure lung tissues.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic and Pharmacodynamic Efficacy of Intrapulmonary Administration of Ciprofloxacin for the Treatment of Respiratory Infections

Chono

Tanino

Seki

et al. 2007

Drug Metabolism and Pharmacokinetics

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The pharmacokinetic and pharmacodynamic efficacy of intrapulmonary administration of ciprofloxacin (CPFX) for the treatment of respiratory infections caused by pathogenic microorganisms resisting sterilization systems of alveolar macrophages (AMs) was evaluated by comparison with an oral administration. The time-courses of the concentration of CPFX in AMs and lung epithelial lining fluid (ELF) following intrapulmonary administration of CPFX solution to rats (200 microg/kg) were markedly higher than that following oral administration (10 mg/kg). The time-course of the concentrations of CPFX in plasma following intrapulmonary administration was markedly lower than that in AMs and ELF. These results indicate that intrapulmonary administration is more effective in delivering CPFX to AMs and ELF, compared with oral administration, in spite of a low dose and it avoids distribution of CPFX to the blood. In addition, the antibacterial effects of CPFX in AMs and ELF following intrapulmonary administration were evaluated by pharmacokinetics/pharmacodynamics analysis. The concentration of CPFX in AMs and ELF-time curve (AUC)/minimum inhibitory concentration of CPFX (MIC) ratio and the maximum concentration of CPFX in AMs and ELF (Cmax)/MIC ratio were markedly higher than the effective values. The present study indicates that intrapulmonary administration of CPFX is an effective technique for the treatment of respiratory infections.

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Lipid-Free Total Parenteral Nutrition and Macrophage Function in Rats

Nussbaum

Li²,

Ogle³

et al. 1991

Arch Surg

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Certain lipids are immunosuppressive when used for nutritional support, while other lipids and nutritional additives may enhance immunologic function. We hypothesized that total parenteral nutrition (TPN) may be immunosuppressive irrespective of lipids. Twenty-four rats underwent central vein catheterization and received either intravenous saline solution and oral chow or TPN alone. At 7 or 14 days, the animals were killed. Splenic and bone marrow macrophages were isolated and cultured in either M199 medium alone or were stimulated with Escherichia coli lipopolysaccharide. The supernatants were tested for prostaglandin E2 and C3. The splenic prostaglandin E2 levels were significantly higher in the TPN group following lipopolysaccharide stimulation at 7 days but not at 14 days. Administration of TPN to rats, even without lipids, may be immunosuppressive through the release of prostaglandin E2 from splenic macrophages following a septic challenge. This effect appears to be abolished after 14 days of TPN infusion.

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The production of C3, PGE2 and TxB2 by splenic, alveolar, and peritoneal guinea pig macrophages

Cited by 16 publications

References 24 publications

Prostaglandin and the Suppression of Phagocyte Innate Immune Responses in Different Organs

Prostaglandin and the Suppression of Phagocyte Innate Immune Responses in Different Organs

Pharmacokinetic and Pharmacodynamic Efficacy of Intrapulmonary Administration of Ciprofloxacin for the Treatment of Respiratory Infections

Lipid-Free Total Parenteral Nutrition and Macrophage Function in Rats

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