The Production of Coenzyme Q10 in Microorganisms

Cluis, Corinne P.; Pinel, Dominic; Martin, Vincent J. J.

doi:10.1007/978-94-007-5055-5_15

Cited by 21 publications

(17 citation statements)

References 86 publications

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“…Коэнзим Q 10 (кофермент Q 10 , CoQ 10, убихинон) -единственный эндогенно синтезируемый жирорастворимый антиоксидант -выполняет в организме множество различных функций, связанных с его окислительно-восстановительным статусом [1,2]. CoQ 10 представляет собой 2,3-диметокси-5-метил-6-декапренил-1,4-бензохинон.…”

Section: Introductionunclassified

HPLC estimation of coenzyme Q10 redox status in plasma after intravenous coenzyme Q10 administration

et al. 2015

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The pharmacokinetics of the total pool of coenzyme Q10 (CoQ10), its oxidized (ubiquinone) and reduced (ubiquinol, CoQ10H2) forms have been investigated in rats plasma during 48 h after a single intravenous injection of a solution of solubilized CoQ10 (10 mg/kg) to rats. Plasma levels of CoQ10 were determined by HPLC with spectrophotometric and coulometric detection. In plasma samples taken during the first minutes after the CoQ10 intravenous injection, the total pool of coenzyme Q10 and proportion of CoQ10H2 remained unchanged during two weeks of storage at -20°C. The kinetic curve of the total pool of coenzyme Q10 corresponds to a one-part model (R2 = 0.9932), while the corresponding curve of its oxidized form fits to the two-part model. During the first minutes after the injection a significant portion of plasma ubiquinone undergoes reduction, and after 7 h the concentration of ubiquinol predominates. The decrease in the total plasma coenzyme Q10 content was accompanied by the gradual increase in plasma ubiquinol, which represented about 90% of total plasma CoQ10 by the end of the first day. The results of this study demonstrate the ability of the organism to transform high concentrations of the oxidized form of CoQ10 into the effective antioxidant (reduced) form and justify prospects of the development of parenteral dosage forms of CoQ10 for the use in the treatment of acute pathological conditions.

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Section: Introductionunclassified

HPLC estimation of coenzyme Q10 redox status in plasma after intravenous coenzyme Q10 administration

et al. 2015

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“…This strain was found to be stable through the continuous propagations for more than 10 passages. Although, specific CoQ 10 content of UF16 has been improved, it is still less as compared to the other high CoQ 10 producing bacterial strains, with maximum reported value of 11.84 mg g -1 of DCW [2]. Hence efforts are needed to further improve this strain by mutagenesis or metabolic engineering techniques.…”

Section: Mutagenesis Selection Of Mutants and Screening In Shake Flaskmentioning

confidence: 99%

“…Coenzyme Q 10 (CoQ 10 ) is commercially important nutraceutical compound used extensively in supportive treatment of cardiomyopathy, cancer, neurodegenerative diseases and diseases associated with CoQ 10 deficiency [1,2]. Structurally, it is a benzoquinone with chain of ten isoprene units attached to it.…”

Section: Introductionmentioning

confidence: 99%

“…The isolation of CoQ 10 from S. johnsonii has been reported by one group, using improved fermentation process involving feeding of para-hydroxy benzoic acid, a precursor of benzoquinone moiety of CoQ 10 [16]. This strain has not been explored extensively for CoQ 10 studies like few higher producing bacterial strains Agrobacterium tumifaciens (Rhizobium radiobacter) and Rhodobacter sphaeroides [2] due to its low CoQ 10 content, hence the strain improvement work on this strain was undertaken by us [17]. Improving the yields of CoQ 10 in S. johnsonii would be beneficial so as to take the advantage of having more biomass production in yeast fermentation.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Differentiation of Sporidiobolus johnsonii ATCC 20490 and Its Coenzyme Q10 Overproducing Mutant Strain UF16

et al. 2014

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Coenzyme Q 10 (CoQ 10 ) is an industrially important molecule having nutraceutical and cosmeceutical applications. CoQ 10 is mainly produced by microbial fermentation and the process demands the use of strains with high productivity and yields of CoQ 10 . During strain improvement program consisting of sequential induced mutagenesis, rational selection and screening process, a mutant strain UF16 was generated from Sporidiobolus johnsonii ATCC 20490 with 2.3-fold improvements in CoQ 10 content. EMS and UV rays were used as mutagenic agents for generating UF16 and it was rationally selected based on atorvastatin resistance as well as survival at free radicals exposure. We investigated the genotypic and phenotypic changes in UF16 in order to differentiate it from wild type strain. Morphologically it was distinct due to reduced pigmentation of colony, reduced cell size and significant reduction in mycelial growth forms with abundance of yeast forms. At molecular level, UF16 was differentiated based on PCR fingerprinting method of RAPD as well as large and small-subunit rRNA gene sequences.Rapid molecular technique of RAPD analysis using six primers showed 34 % polymorphic fragments with mean genetic distance of 0.235. The partial sequences of rRNAgene revealed few mutation sites on nucleotide base pairs. However, the mutations detected on rRNA gene of UF16 were less than 1 % of total base pairs and its sequence showed 99 % homology with the wild type strain. These mutations in UF16 could not be linked to phenotypic or genotypic changes on CoQ 10 biosynthetic pathway that resulted in improved yield. Hence, investigating the mutations responsible for deregulation of CoQ 10 pathway is essential to understand the cause of overproduction in UF16. Phylogenetic analysis based on RAPD bands and rRNA gene sequences coupled with morphological variations, exhibited the novelty of mutant UF16 having potential for improved CoQ 10 production.

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“…The advantage of an all-trans isomeric form is that it is structurally identical to CoQ 10 that is endogenously made in the human body, making it biologically active. 15 Alternatively, there is a lowercost semichemical synthesis that uses a tobacco by-product, solanesol, which contributes tyrosine to the ring structure and the phytyl side chain. This production method contains cis-and trans-isomers that give the isoprenoid tail a bent shape rather than a straight shape.…”

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confidence: 99%

Potential role of coenzyme Q<sub>10</sub> in health and disease conditions

Rodick¹,

Seibels²,

Babu³

et al. 2018

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Coenzyme Q 10 (CoQ 10 ), an endogenously produced compound, is found in all human cells. Within the mitochondria, it plays a substantial role in energy production by acting as a mobile electron carrier in the electron transport chain. Outside the mitochondria, it acts as an excellent antioxidant by sequestering free radicals and working synergistically with other antioxidants, including vitamin E. Dietary contribution is limited, making endogenous production the primary source for optimal function. Now widely available as an over-the-counter supplement, CoQ 10 has gained attention for its possible therapeutic use in minimizing the outcomes of certain metabolic diseases, notably cardiovascular disease, diabetes, neurodegenerative disease, and cancer. Research has shown positive results in subjects supplemented with CoQ 10 , especially in relation to upregulating antioxidant capability. Emerging research suggests beneficial effects of CoQ 10 supplementation in individuals on statin medications. CoQ 10 supplementation in individuals participating in strenuous exercise seems to exert some beneficial effects, although the data are conflicting with other types of physical activity. This broad review of current CoQ 10 literature, while outlining its physiological/functional significance in health and disease conditions, also offers a dietitian's perspective on its potential use as a supplement in the promotion of health and management of disease conditions.

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The Production of Coenzyme Q10 in Microorganisms

Cited by 21 publications

References 86 publications

HPLC estimation of coenzyme Q10 redox status in plasma after intravenous coenzyme Q10 administration

HPLC estimation of coenzyme Q10 redox status in plasma after intravenous coenzyme Q10 administration

Morphological and Molecular Differentiation of Sporidiobolus johnsonii ATCC 20490 and Its Coenzyme Q10 Overproducing Mutant Strain UF16

Potential role of coenzyme Q<sub>10</sub> in health and disease conditions

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